tissue culture is important one

1. A Term Paper Presentation On…. “Clonal Propagation In Ornamental Plants” Course teacher Dr. R.Gnanam Professor Persented by Bhor Sachin Ashok I.D.No.-09-607-001

3. Multiplication of genetically identical copies of a cultivar by asexual reproduction is called a clonal propagation.

4. Applied in crops like potato ,apple, pear, Horticultural crops, ornamental bulbs and in tuberous plants also.

6. Use of tissue culture for micro propagation was initiated by G. Morel(1960) who found this approach in orchid propagation.

8. METHODS OF CLONAL PROPAGATION CLONAL PROPAGATION IN VIVO IN VITRO CUTTING BUD PROLIFERATION BUDDING ORGANOGENESIS LAYERING SOMATIC EMBRYOGENESIS GRAFTING DIVISION & SEPARATION

10. Shoot tip culture

11. Single node culture

15. MAJOR STAGES OF INVITRO CLONAL PROPAGATION

17. Multiplication via somatic embryogenesis.

18. Multiplication via thin cell layer

19. Multiplication by adventitious shoots

21. The Meristem-tip culture is an aseptic culture on artificial medium of the apical dome without leaf primordia. It measures 0.2 to 0.3 mm.

22. Pelargonium, Chrysanthemum etc. are produced from mother plants who were cleaned up by culture of meristems

23. The pathway of regeneration undergoes several steps.

25. Embryos develop through direct embryogenesis or through indirect embryogenesis.

26. Succeeded in ornamental pot plants like chrysanthemum (Dendrathemagrandiflorum)

28. Fig.1.Invitro somatic embryogenesis of Euphorbia pulcherrima. (A) Isolated somatic embryos of E. Pulcherrima (B) Germination of Somatic embryo. (C) Somatic embryos derived plantlets acclimatized in the greenhouse (D) Flowering of somatic embryo-derived plants

30. Reduced cell number in TCL is important developmental process or the morphogenetic programme.

32. But yet the cultures produced from calli are not stable genetically, which will decline plant regeneration capacity.

34. The Nutrient medium

37. Off season production of plants

38. Reduction in life cycle of plant

39. Germplasm conservation

40. Genetic transformation

41. Large scale production of plants

43. This technique is costly than sexual propagation

44. Woody plants does not gives response to clonal propagation

46. Somaclonal variation

47. Cryopreservation

49. CHRYSANTHEMUM

52. Surface sterilization

54. Dark green colur & granular appearance.

56. Subsequent subculturing on media containing BAP.

57. After 5-6 weeks small green leaf Appearance

60. GERBERA

62. Material & Method Seeds soaked in Colchicine (20mg/l for 6 hours) Surface sterilization of Explant Germination of seeds on MS basal medium Crushed germinated seeds are used as Explant Inoculation on MS + Kn,BA+,2,4-D (1-5 mg/l) + BA,NAA,IBA (1-5 mg/l) + 5 mg/l Glutamine,pH-5.8 Incubation for 10 days Transfer of callus on media containing BA,NAA,IBA (1-5 mg/l each) Hardening Media supplemented with Auxins Plantlets are transferred to greenhouse condition Plants shows 98%Survival rate.

63. Orchid

66. Leaf Segment

67. Root segment

68. Inflorescence axis & flower bud

69. Rhizome Segment

71. Cross section (0.3-0.5mm thickness)excised from stem

72. Inoculation on MS media +sucrose(28gm/l)+Different conc. Of BA,Kn &IAA, pH-%.8

73. 20 ml media taken ion to glass bottle &Explant inoculation

74. Transfer of regenerated shoots to GR free media+0-20%sucrose +coconut water (for rooting)

75. Transfer in to pots under G.H. condition

76. Covering of plantlets by polythene bag

79. ROSE

80. REFERENCE ARTICLE . . .

82. S.S.Bohojwani and M.K. Razdan ; Plant tissue culture : theory and practice ; ELSEVIER Amsterdam-Oxford-New York-Tokyo (1983); page no; 313-343.

83. V K Srivastava , S Chadrasekhar; Commercialization of biotechnology for agriculture and aquaculture ; oxford & IBH publisher ; page no:215-221.

84. www.springerlink.comREFERENCES . . .

85. CONCLUSION. . .

86. DISCUSSION . . .

87. THANK U . . .