1. BIOSINTESIS PROTEIN

3. mitochondria
cell membrane
lysosomes
Golgi
rEPR (ribosomes)
sEPR
centrosome
nucleolus
nucleus
cytoplasm

4. Organelle Major functions
Cell membrane Cell integrity, signal transduction
Nucleus Replication, transcription
centrosome Ribosome synthesis (sub units)
cytoplasm Metabolic pathways, signaling
mitochondria ATP synthesis, DNA
ribosomes Protein synthesis
Lysosomes Intra celullar degradation
rEPR Protein synthesis (export)
sEPR Lidpid synthesis, membranogenesis
Golgi Protein transport (addresing)

5. I1 I2
I - introns (non-coding)
promoter
enhancer
silencer
STRUCTURE
REGULATI
ON
REGULATION
E1 E2 E3
E - exons (coding)
5‘ 3‘

6. Gene
cDNA (mRNA)
- 5‘-flanking region, structural part, 3‘-flanking
- exons (coding) and introns (non-coding)
- no real start, no real stop
- 5‘-UTR, coding region, 3‘-UTR
- no uncoding region between start and stop
- start (cap-site) and stop (poly-A tail)

7. pre-mRNA
mRNA
DNA E1 E2 E3
transcription + capping
polyadenylation
AAAAAA
splicing
AAAAA
start stop
termination
signals
poly A
signal
CAP
start stop

8. ribosomal RNA (rRNA)
5 S, 5.8 S (160 bp), 18 S (2000 bp) and 28 S (5000 bp) RNA
ribosome structure
transfer RNA (t-RNA)
small RNA species with characteristic secondary structure
(anticodon, amino acid binding)
amino acid transfer
messenger RNA (mRNA)
matrix for protein synthesis at the ribosome, different
length
translation

9. AUG
(start)
UGA
(stop)
pre-mRNA
5‘ 3‘
E1 E2 E3
gene
I1 I2
PAS
splicing sites
mRNA PAS
start stop
5‘-UTR 3‘-UTR
AAAAAAA
CAP
TR

10. AAAAA
CAP
TR
start
codon
stop
codon
5‘-UTR 3‘-UTR
structural part
regulatory parts

11. degenerated code: several triplets code
for one amino acid,
only methionine (start) has
just 1 codon
stop
stop
stop

12. Bahasa gen
bersifat
universal

13. KODON ATAU SANDI GENETIK
TRIPEL NUKLEOTIDA

14.  DARI MCB
 DARI SI NTESIS PROTEIN LEHNINGER 2000
 DARI HRNYAHYA

15.  PERHATIKANTEORI DOGMA SENTRAL, PRODUK AKHIR GEN
ADALAH PROTEIN
 DNA SEBAGAI MATERI GENETIK
 mRNA SEBAGAI PEMBAWA INFORMASI GENETIK
 SINTESIS PROTEIN TERJADI DI RIBOSOM
 mRNA MEMBAWA CODON, SEDANGKAN tRNA-MEMBAWA
ANTI CODON
 PESANAN ASAM AMINO DIKODE OLEH CODON mRNA
 ASAM AMINOYANG SATU DENGANYANG LAIN DIRANGKAI
DENGAN IKATAN PEPTIDA
 START CODON SELALU DIMULAI DENGAN METIONIN DAN
SINTESIS PROTEIN DIAKHIRI DENGAN STOP CODON

26. TABEL KODON

30. Amplification of Message byTranslation
From 1 spliced mRNA molecule many protein molecules are made.

31. SINTESIS PROTEIN SELALU DIMULAI DENGAN METIONIN

33. TERMINASI DENGAN STOP CODON

35. primary structure
secondary structure
tertiary structure
quatery structure
amino acid sequence
spatial arrangement of amino acids
within a poly-
peptide chain (a-helix, ß-sheet,
unstructured turns)
spatial arrangement of
secondary structural elements
subunit interactions

36. there are about 20 proteinogenic amino acids

37. Proteins are Made of AAs
Connected by Peptide Bonds
• Ikatan peptida bersifat ikatan rangkap
spt 2 lembar kertas yang dilem (SRTLH)
arti dipeptida, tripeptida, jml AA & ikt pep
• Pada praktikum: berwarna ungu

38. I M U N O B L OT I N G
1 2 3 4 5 6 7
66,0 kDa
28
20,1 kDa
45,0 kDa
29,0 kDa
14,2 kDa
55,0 kDa
84,0 kDa
97,4 kDa
Gambar 6. Hasil elektroforesis protein dari biakan E.coli rekombinan pembawa
gen penyandi protein solubel 28 kDa (GRA2) T. gondii yang diimunobloting dengan
antibodi poliklonal terhadap protein solubel takizoit T. gondii.
1. protein standar, 2. lisat protein dari E.coli dengan pewarnaan amidoblack,
4, 5, 6 dan 7 adalah hasil imunobloting protein dari klon rekombinan, sedang
3 adalah protein dari klon berwarna biru ( bukan rekombinan)
Koloni yg mengeksp protein
rekombinan diimunobloting dg
Ab poliklonal thd Prot sol T.
gondii
Pita prot rek gb pita berbeda dg
prot non rek, intensitas warna
berbeda,yg dominan prot rek dg
BM 28 kDa yg merup hasil eksp
cDNA
kDa
kDa

39. • STRUKTUR PROTEIN

40. Proteins Form Complex 20 Structures
• R Groups define the
behavior of an amino
acid and the structure
a protein domain folds
into.
• Acidic
• Basic
• Neutral
• Hydrophobic
• Hydrophilic
• Electrostatic

41. MACAM DAN FUNGSI PROTEIN
1. Protein Regulator / Bioregulator ( Hormon)
2. Biokatalisator ( Enzim )
3. ProteinTransport: Hb, Alb,
Lipoprotein,Transferin
4. Protein Kontraktil: aktin dan miosin
5. Protein Struktural:
kolagen,tubulin,keratin,glikopr
6. Protein Pelindung: Ig, interferon,
perforin,IL

42. TERIMA KASIH
Thank you for your
kind attentions ..