Amphibian ranavirus dynamics in
an industrially altered landscape
Danna M. Schock
2nd International Symposium on Ranavirus...
Map from the Washington Post
Fort McMurray, Alberta
Oilsands, boreal forest, big money & big politics
RV dynamics like prevalence
& frequency of outbreaks
Habitat
characteristics, Wetland
classification
Contaminant levels in...
Establishing field sites – 2012 & 2013
1)Wood frogs breeding at the site
2)In close proximity to air monitoring
stations
3...
Atmospheric deposition and pulses of contaminants into amphibian
breeding ponds in the spring
Photo: Dave Cooper, Calgary ...
wood frogs boreal chorus frogs
Canadian toads
Ranaviruses detected widely in
wood frogs
12/27 (44%) sites positive at
least once from 2010 – 2013
Positive wetlands from...
Ranaviruses detected widely in
wood frogs
Ranavirus-related die-offs at
remote sites (helicopter access
only) and highly d...
Ranaviruses detected widely in
wood frogs
The majority of carcasses are
tadpoles Gosner stages 37 - 40
All positives seque...
Data collected
Basic information
• Species
• Snout-Vent-Length
• Gosner stage (tadpoles), weight (terrestrials)
• Physical...
Narratives of 3 wetlands
• Jetliner – loss of entire age class
• Tower Road – repeated die-offs, no apparent change in
pop...
Distribution of mass of
terrestrial wood frogs in
2013 at 6 intensively
studied sites.
Mass (g)
Jetliner
#ofindividuals
Jetliner
Jetliner
Wood frog egg masses at Jetliner May 2011
Jetliner Pond
Mass (g) of terrestrial
wood frogs 2011 - 2013
2011
2013
2012
#ofindividuals
Mass (g)
Distribution of mass of
terrestrial wood frogs in
2013 at 6 intensively
studied sites.
Mass (g)
Tower
Road
#ofindividuals
Tower Road
Tower Road
Distribution of mass of
terrestrial wood frogs in
2013 at 6 intensively
studied sites.
Mass (g)
Gateway
#ofindividuals
Gateway
Gateway
Size at metamorphosis is another indication of population health
Snout-vent length (mm) of Gosner Stage 44
wood frogs in 2...
A role for environmental transmission?
Unrelated 2011 lab study:
Only wood frog tadpoles in reference wetland
water and ag...
A role for invertebrates in ranavirus transmission?
• Impede transmission by scavenging carcasses quickly?
• Facilitate tr...
What about nutrient/energy cycles in these wetlands?
• Die-off years vs non-die-off years?
• Different invertebrate specie...
Data to come:
PCR screening of 2,200+ tissue
samples for ranavirus
Water and tissue contaminant
analyses
Bio-markers of (c...
Amphibian ranavirus disease dynamics in an industrially altered landscape
Amphibian ranavirus disease dynamics in an industrially altered landscape
Amphibian ranavirus disease dynamics in an industrially altered landscape
Amphibian ranavirus disease dynamics in an industrially altered landscape
Upcoming SlideShare
Loading in …5
×

Amphibian ranavirus disease dynamics in an industrially altered landscape

754 views

Published on

2013 International Symposium on Ranaviruses
by Danna Schock

Published in: Education, Lifestyle, Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
754
On SlideShare
0
From Embeds
0
Number of Embeds
9
Actions
Shares
0
Downloads
6
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Amphibian ranavirus disease dynamics in an industrially altered landscape

  1. 1. Amphibian ranavirus dynamics in an industrially altered landscape Danna M. Schock 2nd International Symposium on Ranaviruses Knoxville TN, 27-29 July 2013
  2. 2. Map from the Washington Post Fort McMurray, Alberta Oilsands, boreal forest, big money & big politics
  3. 3. RV dynamics like prevalence & frequency of outbreaks Habitat characteristics, Wetland classification Contaminant levels in amphibian tissues and breeding ponds Population biology & biomarkers of (chronic) stress Proximity to disturbancesCan wood frogs be used to monitor ecosystem health in the oil sands?
  4. 4. Establishing field sites – 2012 & 2013 1)Wood frogs breeding at the site 2)In close proximity to air monitoring stations 3)Geographically representative of the landscape* 4)Safe to work Priority placed on identifying wetlands suitable for long-term monitoring of the oil sands region of Alberta.
  5. 5. Atmospheric deposition and pulses of contaminants into amphibian breeding ponds in the spring Photo: Dave Cooper, Calgary Herald Snow sampling March 2013
  6. 6. wood frogs boreal chorus frogs Canadian toads
  7. 7. Ranaviruses detected widely in wood frogs 12/27 (44%) sites positive at least once from 2010 – 2013 Positive wetlands from Fort Resolution to Fort McMurray ● PCR and/or characteristic lesions and carcasses (~2,200 samples still to screen, including most samples from 2013)
  8. 8. Ranaviruses detected widely in wood frogs Ranavirus-related die-offs at remote sites (helicopter access only) and highly disturbed sites Die-offs take place last week of June/first week of July ● irrespective of type of site ● irrespective of spring thaw
  9. 9. Ranaviruses detected widely in wood frogs The majority of carcasses are tadpoles Gosner stages 37 - 40 All positives sequenced to date are FV3-like ● MCP4/5 primers ● but see Schock et al. 2008 Copeia re “hidden” variability of northern isolates ● additional characterization in progress by J. Brunner
  10. 10. Data collected Basic information • Species • Snout-Vent-Length • Gosner stage (tadpoles), weight (terrestrials) • Physical abnormalities Tissue samples • toe/tail clips (several times/year, screen for RV, Bd) • lethal whole body, once/year, screen for contaminants and evidence of stress Water quality • pH & conductivity (handheld meters) • In-depth analysis (3x per active season) including hardness, and 26 metals, 74 PAH, and 60 NA
  11. 11. Narratives of 3 wetlands • Jetliner – loss of entire age class • Tower Road – repeated die-offs, no apparent change in population biology (yet?) • Gateway – why hasn’t ranavirus been detected here (yet)? Results – an evolving story
  12. 12. Distribution of mass of terrestrial wood frogs in 2013 at 6 intensively studied sites. Mass (g) Jetliner #ofindividuals
  13. 13. Jetliner
  14. 14. Jetliner
  15. 15. Wood frog egg masses at Jetliner May 2011
  16. 16. Jetliner Pond Mass (g) of terrestrial wood frogs 2011 - 2013 2011 2013 2012 #ofindividuals Mass (g)
  17. 17. Distribution of mass of terrestrial wood frogs in 2013 at 6 intensively studied sites. Mass (g) Tower Road #ofindividuals
  18. 18. Tower Road
  19. 19. Tower Road
  20. 20. Distribution of mass of terrestrial wood frogs in 2013 at 6 intensively studied sites. Mass (g) Gateway #ofindividuals
  21. 21. Gateway
  22. 22. Gateway
  23. 23. Size at metamorphosis is another indication of population health Snout-vent length (mm) of Gosner Stage 44 wood frogs in 2013 F5,159 = 17.287, P < 0.01a a b
  24. 24. A role for environmental transmission? Unrelated 2011 lab study: Only wood frog tadpoles in reference wetland water and aged tap water became infected. FV3: apparently “killed” by something in the OSPM water? Tadpoles in the OSPM water: 85%+ survival Gateway
  25. 25. A role for invertebrates in ranavirus transmission? • Impede transmission by scavenging carcasses quickly? • Facilitate transmission or persistence – leeches ?
  26. 26. What about nutrient/energy cycles in these wetlands? • Die-off years vs non-die-off years? • Different invertebrate species assemblages?
  27. 27. Data to come: PCR screening of 2,200+ tissue samples for ranavirus Water and tissue contaminant analyses Bio-markers of (chronic) stress – metabolomics, CORT Other fitness correlates – triglycerides & glycogen PCA and Regression analyses

×