1. The Sertoli Cell Junction
Dynamics
Falana, Benedict Abiola
Department of Anatomy,
CMUL.
Matriculation Number: 109091016
ANA 902
25th February 2014
1
5. Introduction: SPERMATOGENESIS
• 1. Spermatogonia
– Proliferate by mitotic divisions
to provide stem cells & cells
which will proceed through
spermatogenesis (1º
spermatocytes)
• 2. Spermatocyte
– diploid cells (2n) give rise to haploid
cells (1n)
– 1º spermatocytes enter Meiosis
I to form 2º spermatocytes
which then enter Meiosis II and
result in spermatids
• 3. Spermatids
– spermatid differentiation into
spermatazoa
5
8. Questions
• What is the mechanism of by which
developing germ cell traverse the
epithelium?
• What signalling event determine the time of
for germ cell to translocate from one site to
the other?
• What interactions between Sertoli and germ
cells take place during translocation?
• What are the events leading to formation
and disruption of Sertoli-germ cell
junctions?
9. Ultrastructure of the Sertoli Cell: Irregular nuceli, Mitochondria, lipid and RER are prominent.
Courtesy : www.naturescience.com
• Ultrastructure of the Sertoli Cell: Irregular
nuceli, Mitochondria, lipid and RER are
prominent
10. LITERATURE REVIEW
On going Controversies.
• A Single Spermatogoonium (2n)
gives rise to Eight Spermatids (1n)
(de Krester & Kerr 1988).
• Preleptotene and Leptotene
spermatocytes. (Zipper Theory)
• BTB.
(Byers 1993; Pelletier 2001).
15. • CX43 alters Occludin expression in the rat ST.
(Gerber 2014)
• Spectrin and Plectin sorrounds the actin cuffs
of apical tubulolobular complexes in the rat.
(Aristaeuss de Asis 2013)
16. • Clathrin/actin-base endocytic
machinery is associated with
junction turnover in ST.
(Vogl 2014).
• Dicer is required for sertoli cell
function and survival.
(Papaioannou 2009; Kim 2010;Hensley et al., 2014).
16
17. • RIA 14 regulates F-actin dynamics
at the ES.
(Qian 2013).
• CAM-I enhances Germ-Sertoli cell
interaction in spermatogenesis.
(Lewis 2005; Wakayama & Iseki 2009)
17
18. • FAK is a regulator of F-actin
dynamics (Li 2013)
• Disruption of Sertoli-germ cell
adhesion function limited to
adherens junctions.
(Xia et al., 2005)
18
19. • Taurine disrupts the morphology and
Ultrastructure in the testis of mice.
(Abdel-Moneim 2013)
• TGF-beta3 regulates anchoring junction
dynamics via the Ras/ERK signaling
pathway.
(Xia 2005)
19
20. • Ultrastructural changes of the
Sertoli and Leydig Cells following
STZ induced diabetes.
~ Body Weight ↓ (P˂0.05)
~ Testicular Weight↓(P˂0.05) in
diabetic rats compared with
controls.
(Kianifard 2012)
21. • Β- Conglycinin reduces the tight
junction occludin and ZO-I
expression in IPEC-J2.
(Brosnan 2012 ; Zhao et al., 2014)
21
23. • Claudin-3 and Claudin-5 folding
and assembly into tight junctions
are controlled by non- conserved
residues in TM3 and ECL2
segments.
(Rossa et al., 2014; Shabazi 2014).
23
24. • Immunohistochemical analysis of
Histone H3 modifications in germ
cells during mouse
spermatogenesis.
(Song et al., 2011).
24
25. • TRAF4 impedes the formation of
tight junction integrin-linked
kinase (ILK) Talin-1.
(Rosseau et al., 2014).
25
26. DISCUSSION
STRUCTURE
Blood-testis barrier
Controls the entry and exit of
nutrients hormones and other
chemicals into the tubules
Makes the ad luminal part of ST, an
immune privileged site.
(Wakayama 2009; Kopera 2010)
26
32. BTB
FUNCTIONS
Allow sertoli cells to control the
ad- luminal compartment.
Regulates the chemical
composition of luminal fluid.
Sensitive to trauma and
Autoimmune response 32
35. AJs
• Zona adherens
• Located at site of cell-cell interaction
• Actin-linked
• Attaches ECM
• Posses specific transmembrane
receptors of the integrin family
• Adhesive function
45. ES
CCMPC
CATENIN ( α-β-ϒ-) :
Cytoplasmic proteins
Bind to the C-terminus of the type I/II and
desmosomal cadherins
Regulates cadherin-mediated adhesion via
the actin and intermediate filament
cytoskeletons
( Das et al., 2014)
45
46. ES
CCMPC
(Wine & Capin 1999;Johnson & Boekelheide 2002; Lee et al., 2003, 2004; Yan et
al., 2008a; Delva & Kowalczyk 2009; Yan et al., ; 2008b; Izumi et al., 2006).
• Best studied actin-based adhesion
unit.
46
50. ES
ILMPC
Functions
• Connects a cell to ECM
(Margadant et al., 2008; Geiger et al., 2009)
• Constituents of the BM
(Miner & Yurchenco 2004; Miner 2008)
50
51.
52. SUMMARY
• The integrity of the BTB cannot be
compromised.
• TJ on and off switch
• Signal transduction is very essential and
crucial in tight junctional dynamics.
52
53. CONCLUSION
• Changes in secretory activity of either Sertoli
or germ cells initially take place at the level
of cell junctions.
• Biology of desmosome-like junctions and ES
needs to be thoroughly investigated.
• Basis of male contraception and infertility
treatment.
53
54. Acknowledgement
• Sincere gratitude to my able supervisors
Dr. Francis Duru and Dr. Abraham Osinubi for their
mentorship role.
• I also want to appreciate my Teachers Dr.
Ibeabuchi, Dr. Oremosu, Dr. Kusemiju, Dr.
Dosumu, Dr. Olabiyi, Dr. Gbotolorun , Dr. Yama for
their assistance during preparation for the review.
• A big thanks to fellow Postgraduate students and the
non-teaching staff of the Department.
54
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