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ENDODONTIC
DIAGNOSIS
Deepthi P.R.
1st year MDS
Dept of Conservative
Dentistry & Endodontics
CONTENTS
 Introduction
 Diagnosis
 Diagnostic method
 Medical history
 Drugs & medication history
 Dental history
 Subjective symptoms
 Clinical observations
 Clinical tests
Introduction
 Thorough knowledge of other sciences
 Diagnosis & Treatment planning
 Pain of non odontogenic origin
 Accurate database:
 Medical & dental history
 Clinical examination & relevant tests
 Making & interpreting appropriate
radiographs
Diagnosis
 ‘The art and science of detecting
deviations from health and the cause and
nature thereof’
 Differential diagnosis: ‘The process of
identifying a condition by comparing the
symptoms of all (or other) pathologic
process that may produce signs and
symptoms ’
Glossary of endodontic terms. 7th ed. Chicago: American
Association of Endodontists;2003
Diagnosis
 Inability to test/ image the tissue directly
 Indirect interpretation of response to
stimuli
 Determine teeth free of disease rather
than diseased
Newton et al. JOE- Volume 35, Number 12, December 2009
Diagnostic method
METHODS
Pulp testing
Palpation
Percussion
DIAGNOSTIC APPROACHES
Bite test
Test cavity
Staining/ Transillumination
Selective anesthesia
Radiography
Dental history/
Medical history
Evaluation of pain
signs/ symptoms
Newton et al. JOE- Volume 35, Number 12, December 2009
Surgical Sieve
Pitt Ford & Rhodes. Endodontics- Problem solving in Clinical Practice
• Biographical
details
• Medical history
• Chief complaint
• History of present
complaint
• Dental history
• Social history
• Extraoral
examination
• Intraoral
examination
• Special tests
• Radiographs
• Diagnosis
• Treatment plan
s
A sample form used in diagnosis and treatment planning. (Adapted
from Krell K, Walton R: Odontalgia: diagnosing pulpal, periapical, and
periodontal pain. In Clark J, editor: Clinical dentistry, Philadelphia,
1987, Harper & Row.)
Medical history
 Treatment: harmonious with general
health
 Impact of the patient’s health on the
dental operating team
 Alterations in the usual course of
treatment
 Name & contact of physician
Rheumatic fever
Potential for SBE after bacteremia
Antibiotic premedication:
 Artificial heart valves: Same antibiotic
coverage: rheumatic fever
Pulp
extirpation
Filing beyond
the apex
Rubber dam
placement
Initial
appointment/
Surgical
appointment
Possibilty of
going past
the apex
Periapical
lesion
Coronary Artery disease
 Physician consultation: anticoagulant
 Non surgical treatment preferred
 Mild / moderate analgesics
 Brief recess: more than one tooth- single
appointment
 Substernal pain: dressing placed &
treatment terminated; referred to
physician
Hypertension
 Injection of L/A solutions < 30sec/ml
 Warm anesthetic solutions: few minutes
before injection
 Tranquil mood created- minimal mention
of complications & failures
 Hypnotic premedication: consultation
with physician
Hypertension
 Avoid G/A & no more than 3 anesthetic
carpules
 Morning appointments preferred
 Night time premedication with early
appointments
 Total appointment time not > 1 hour
 Terminate when patient is stressed
Diabetes
 Retarded healing: postop radiographs
 Antibiotics: Infection/ surgery
1yearPre op 6 months 2 years1.5 years 3 years
Diabetes
 Alteration in blood glucose levels:
physician consultation
 Epinephrine avoided: Increase in blood
glucose levels & tissue sloughs post
surgery
 Levonordefrin
 Barbiturates & sedatives cautiously used
Diabetes
 Longer & deeper
anesthesia
 L/A preferred
 Appointments: soon after meals
 Differentiate & manage hypoglycemia/
hypoinsulinism
• Mepivacaine
+Levonordefrin
• Propoxyphene+ Procaine
+Levarterenol bitartarate
Hepatitis
 Resistant to normal sterilization
 Intracanal instruments: discarded after
use
 Avoid drugs detoxified in the liver:
Halothane,Erythromycin
 Cautious- Paracetamol
Blood diseases
 Internal bleeding: L/A administration
 Avoid injections: necrotic pulp
 Vital pulp:
First appt.
•Access to the
cavity
•Dressing
Second appt
•A week later
•Fixed pulpal
tissue removed
•Dressing
replaced
Process
continued: vital
tissue removed
Canals enlarged
& filled
Blood diseases
 Rubber dam: Notches- labial & lingual
surfaces
 Gingival bleeding: do not treatment
without systemic diagnosis
 Infectious mononucleosis:
 Avoided in acute stage
• Pain
• Exacerbations
• Exaggerated
response to
drugs
Joint
replacement
prostheses
 Bacteremia
 Antibiotic
coverage
 Painful joint after
procedure:
orthopedic
surgeon consulted
 Longer than usual:
desirable results
 Hypersensitivity states:
drugs only when
absolutely indicated
 Avoid new/ unusual
drugs
 HIV: transmission
avoided- proper
asepsis
Other serious
Diseases
Recent change in weight
Weight loss
 Dieting
 Loss of appetite
 Systemic diseases
Weight gain
 Psychogenic reasons
 Hormonal
disturbances
 Pregnancy
 Protect exposed
tooth surfaces after
endodontic therapy
 Salt & water
retention
Psychologic problems
 Physical problems: tendency towards
anxiety
 Patients on Tranquilizers/ antidepressants
Converted a
psychologic
condition to
physical problem
Severe fears &
anxieties –
treatment difficult
• No relief with treatment
• Pulpal problem
suspected: suspicious
oral conditions
• Friendly and firm
• Instruments: out of sight
• Informative booklets
• Smooth & painless initial
visit
Others
Hyperthyrodism
 No epinephrine
 Increase sedative if needed
Ulcers
 Avoid aspirin & if on antacids- avoid
tetracycline
 Use Penicillin V if needed
Alcoholic
 Cautious with sedatives
 Aspirin avoided
Drugs & Medication therapy
 Physical condition & effects of
medications
 Adverse reactions
 Questionnaire format
 Unaware of Drug’s contents : Mosby’s
Drug Consult/ physician
 History of allergy: minimum inter
appointment time & well monitored
Drugs & Medication therapy
 Steroid therapy: intratreatment pain &
exacerbations , infections
 Appointments: maximum 3 days apart
 Vital: 2 sitting & Necrotic: 3 sitting – 1 week
period
 Surgery- Antibiotic therapy & steroid
dose
Drugs & Medication therapy
 Aspirin: bleeding after surgery
 Avoid- Blood dyscrasia, anticoagulant,
renal transplant, gout
 Caution- Asthma, Diabetes, Last month of
pregnancy
 Tranquilizer therapy: unusual reactions to
prescribed hypnotics/ narcotics
 Physician consulted
Drugs & Medication therapy
• CNS stimulant:
increase
sedative dose
• Sulfonamides:
avoid
procaine
Antidepressants:
Cautious
• GA
• Narcotics
• Antisialagogue
Tetracycline:
• Antacids
• Penicillin
Barbiturates :
cautious
• Dilantin
• Griseofulvin
• Steroids
Dental history
 Patient’s objective for treatment- clear
 Appreciation for dental treatment
 Experiences with previous dentist
Pain
relief
Check
up
Oral
systemic
relation
CosmeticsMasticatory
inefficiency
Dental history
 Chief complaint & its history
 When was it last restored?
 Pulp capping/ Pulpotomy/ large
restoration in the same
 Sharp blow/ accident
 Swelling/ gum boil
 Drainage
Subjective symptoms
 Is the pain still present?
 What type? (Sharp/ dull)
 Throbbing?
 Intermittent/ Continuous?
 Aggravated by: cold, heat, pressure,
mastication, lying down, sweet, sour?
 How long does it last?
Clinical Observations
 Extraoral swelling
 Lymph node
involvement
 Intraoral
involvement
 Fistula
 Tooth discoloration
 Traumatic injuries:
fractures
 Deep carious lesion
 Recurrent caries
beneath a
restoration
 Extensive
restoration
 Developmental
defects of teeth
 Gingival recession
Clinical Tests
Diagnostic tests:
1. EPT
2. Thermal tests
3. Percussion
4. Palpation
5. Mobility
6. Periodontal
evaluation
7. Occlusal evaluation
8. Radiograph
Selective tests for
Difficult Diagnostic
Situations:
9. Test cavity
preparation
10. Anesthetic test
11. Transillumination
12. Biting
13.Staining
14. Gutta percha point
tracing with radiograph
Extraoral
examination
 External facial form & features
 Fistulae, erythema, pallor
 Neurologic examination: motor function,
sensitivity, movement
 Lymph nodes: inflammatory,
infectious, tumor like disorders
Intraoral examination
Soft tissue examination:
 Swelling/ fistula
Intraoral examination
 Crown discoloration: non vital pulp,
removal of discolored dentin, use of
chlorinated soda
 Deep carious lesions/ fractures: visual
examination & probing
Percussion test
 Simple, but useful
 Inflammatory condition of the apical
periodontium
 First clinical indications of apical
periodontitis
Percussion test
 Symptomatic apical periodontitis: more
sensitive
 Pulpal diseases: not reveled unless apical
periodontium is involved
 Periodontal/ endodontic etiology,
occlusal trauma, combination with
marginal periodontitis
 Horizontal percussion
Percussion test
 Firm digital pressure/ handle of instrument
like mouth mirror: tap in a vertical
direction
 Patient bite on Tooth Slooth/ Cotton swab
 Several teeth repeatedly
 Random order
Palpation
 Vestibular region: apical region of the root
tips
 Tenderness, swelling, fluctuation,
hardness, crepitation
 Tip of index finger
 Usefulness increase
with skill &
clinical experience
Mobility
 Moving in a buccal- lingual direction
 Index finger on the lingual surface &
lateral force applied with instrument
handle from buccal surface
 Using two fingers
Mobility
Miller’s index:
 Class 1- First distinguishable sign of
greater- than- normal movement
 Class 2- Movement of the crown as much
as 1mm in any direction
 Class 3- Movement of the crown more
than 1 mm in any direction and/or vertical
depression/ rotation of the crown in its
socket
Periodontal probing
 Endodontic & periodontic lesions mimic
each other concurrently
 Record probing depths: periodontal
health & prognosis
 Entire circumference probed
Periodontal probing
Narrow isolated probing defects:
 Periodontal disease
 Sinus- like trap following periapical
pathosis
 Vertical groove defect
 Cracked teeth
 Vertical root fractures
 External root resorption
Tests for Cracked Tooth
Syndrome
Transillumination
 Fiberoptic light
 Coronal cracks/ vertical root fractures
 Minimal background lighting
 Light placed on varied surfaces of
coronal tooth structure/
root after flap refection
Transillumination
 Light traverses fracture lines-
visually detected
 Fractured Segment near the
light appears brighter
Dye staining
 Dye penetrates fracture line
 Demonstrates fractures
 Apply – internal surfaces of cavity
preparation/ access opening
 Leave it in place for a week
 Iodine/ methylene blue dye
Dye staining
3 methods:
Remove restoration:
 Direct revealing of fracture line
 Dye incorporated into ZOE
mixture & placed
 Patient chews on disclosing tablet
Bessner & Ferrigno. Practical guide to Endodontics
Bite test
 Wooden stick- opposing
teeth
 Tooth slooth
 Patient bites down & pain elicited
upon release
 Rubber dam sheet- cracked cusp
flexes
Pulp tests
 Major & essential part of diagnostic
process
 Reproduce patients symptoms, diagnose
diseased tooth & disease
 2 independent diagnostic test results
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Pulp tests
 *Ideal technique: non invasive, painless,
standardized, reproducible, reliable,
inexpensive, easily completed & objective
*Chambers. 1982
Pulp sensibility tests
• Thermal tests
• Electric pup tests
• Test cavity
Pulp vitality tests
• Laser doppler flowmetry
• Pulse oximetry
• Tooth temperature
measurement
Pulp sensibility tests
 Pulp nerve fibers respond – external
stimulus
 Thermal/ Electrical / Direct dentine
stimulation
 Do not indicate the health status &
unreliable responses
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Pulp sensibility tests
 No indication of vitality: intact vasculature
 Correlation between test results &
necrotic pulps only*
 Assess whether necrotic or not & does
not quantify the degree of disease
 Useful : identifying diseased tooth
*Seltzer et al.1963, Tyldesley & Mumford 1970, Dummer et al, 1980
Pulp sensibility tests
Preferred sequence:
 Tests repeated after 1’ recovery time
 Thermal tests: no method to assess how
responsive the tooth is or to compare with
previous result
 EPT: numerical display- not essentially
reproducible
Disease free
contralateral
teeth
Opposing
teeth
Presumably
healthy teeth-
same
quadrant
Most
suspicious
tooth
Rationale of the tests
 Sharp, non lingering pain- application of
thermal stimulation: normal
 A - 25% stimulus required to activate C
fibers*
*Virtanen 1985, Hargreaves & Goodis 2002
Thermal tests- Rationale
 Sensory response: not by temperature
changes in receptors
 Hydrodynamic movement of fluid:
dentinal tubules- A fibers
 Cold- faster A fibers: sharp localized
pain
 Heat- slower C fibers: dull long lasting pain
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Electric Pulp Test - Rationale
 Current sufficient to overcome the
resistance of enamel & dentine- stimulate
A fibers
 Sensation felt with gradually increasing
level of current: pulp responsive/ partially
alive
 *Ionic shift in tubules local
depolarization action potential
Pantera et al. 1993
EPT- Rationale
 A fibers: brief sharp sensation/ tingling
 *No blood flow- pulp becomes anoxic &
A fibers cease to function
*Pitt Ford & Patel 2004
Indications
1.Pain in the trigeminal area; referred pain
2. Periodical monitoring of teeth after
trauma
 1-8 weeks lapse before normal response
 EPT: reliable after trauma**
*No response Response : Recovery
Repetitious response :Healthy pulp
Response No response: Degeneration
No response persistent: Necrotic pulp
**Ingle et al 2002,*Bhaskar & Rappaport 1973
Indications
3. Assessment of pulpal health before
restorative procedures
 potential prosthetic abutment
4. Pulp preservation procedures & extensive
restorations
5. Differentiate periapical radiolucencies
from normal anatomical structures & non
odontogenic lesions
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Indications
6. Predict potential anesthetic problems &
evaluation of analgesics
 Cold test: assess pulpal anesthesia
 Preoperative pulp-test performed
 Traditional parameters verified
 Retested with the same test
 Prepared for treatment & level of
anesthesia screened
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
7. Pulp status of transplanted teeth
Indications
8. Le Fort type fractures/ osteotomies
 Normal: 7-11 months after surgery
Limitations
1. Subjective; measure only nerve supply
2. Thermal tests: not effective in substantial
secondary dentine formation
3. Unreliability of tests: Immature apices,
traumatic injuries, more subjectivity in the
young
4. No correlation with the histologic status
(Contrasting results: Hill, 1986)
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Limitations
5. Difficult to administer & inconclusive in
children
6. Weaker response- aged pulp
7. Extensive restorations, pulp recession,
pulp calcification
8. Lack of reproducibility
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Interpretation- Diagnosis
 Immediacy, intensity & duration of
response
 Outcome: never certain
 No particular response- unique to specific
pathologic states
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Clinically Normal pulp
 Mild to moderate transient response to
cold & electrical stimuli
 Response subsides in few seconds on
removal of stimulus
 Do not usually respond to heat tests
Reversible pulpitis
 Thermal stimuli (cold)- sharp pain
 Subsides as soon as the stimulus is
removed/ in few seconds
Irreversible pulpitis
 Thermal changes (cold): sharp pain , dull
prolonged ache- last upto an hour or so
 Valuable: stimulus as reported by patient
applied & pain reproduced & assessed
 EPT: not of value
Pulp necrosis
 Histological state not determined
 Significant relation between lack of
response & pulp necrosis
 No response with EPTs & thermal tests
 No indication of infection expected from
these
Pulp necrobiosis
 Difficult to diagnose
 History : pulpitis
 Pulp tests: necrosis
 Vague response to EPTs, cold tests
Periapical conditions
Acute apical periodontitis
 Maybe associated with pulpitis
 Pulp status assessed before treatment
Acute apical abscess
 Negative
Lateral periodontal abscess
 Positive
Chronic apical periodontitis
 Sequel of infected canal system
False responses
False negative results: Normal pulps that do
not respond to tests
 Calcification: no response to cold; may
respond to high value of current in EPT
 Premedication
 Recent trauma
 Immature apex
 RCT teeth: not expected to respond
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
False responses
 Extensive restorations
 Pulp protecting bases
 High pain threshold
 Activation of fixed orthodontic appliances
 Psychotic disorders
 Defective EPT device/ discharged
batteries/ poor electrical contact
False responses
False positive results: Necrotic pulps
responding to tests
 Conduction of current to adjacent
gingival & periodontal tissues (avoided with
reasonable current strength & proper techniques)
 Moist gangrene, partially necrotic tissue,
infected pulp
 Breakdown products of localized necrosis
False responses
 Calcified tooth structure conducting to
tissue apical to an area of necrosis
 Current conducted to adjacent teeth
through metallic restorations (avoided by
rubber dam / celluloid strips between teeth)
 Inflamed pulp tissue in one canal of a
multirooted teeth with other canals &
chamber necrotic
 Anxious/ young patient
False responses
 More common with EPT than cold test
 EPTs: all teeth; cold tests: multirooted
teeth
 EPT: rare false negative, if more than one
surface used
 Cold test: sometimes, only cervical area
responds
Value of diagnostic tests
 Precision: ‘Tendency of repeated
measurements on the same sample to
yield the same result’
 Variability: Lack of precision
 Accuracy: The extent to which a test
correctly classifies patient’s response
 Sensitivity: The ability of the test to detect
the disease in patients who actually have
the disease
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Value of diagnostic tests
 Specificity: The ability of a test to detect
the absence of a result
 Positive predictive value: The probability
that a positive test result actually
represents a disease positive tooth
 Negative predictive value: The probability
that a tooth with a negative test result is
actually free from the disease
Value of diagnostic tests
 Heat: relatively high sensibility; but least
accurate being the least specific
 Cold test: more accurate than EPT
Thermal tests
 Often inappropriately referred to as
‘Vitality tests’
 More reliable than EPT
 Inexpensive & easy-to- use equipment
 Patient’s pain reproduced
Thermal tests
• Initial cold sensitivity
• Heat sensitivity- continued pulp deterioration
• Disappearance of cold sensitivity
• Cold stimuli might relieve heat induced pain
Damage to hard & soft tissues
of the tooth
 Heat test: more potential to injure
 Tissue freezing: -100c for 5-20’
 Intracellular ice crystal formation &
ischemic necrosis following vascular
injuries
 -220c lowered pulp temperature to 110c:
caused no damage (Langeland et al,
1969)
Damage to hard & soft tissues
of the tooth
 Conflicting reports: Dry ice inducing
enamel cracks
 Delayed cold transfer process: Cold
stimulus applied to necrotic pulps under a
bridge- felt by adjacent tooth
 ‘Film boiling’/ ‘ Leidenfrost phenomenon’:
Insulating layer of CO2 gas around dry
ice, if it falls into mouth
Cold tests
Ice sticks
 0oC temperature
 Not accurate: adult posterior teeth
 Secondary/ reparative dentin deposition
 Testing under crowns/ splints
 Application- 5s : reliable & valid
 Disadvantage: less effective stimulation
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Cold tests
 Freezing water- hypodermic needles’
plastic cover/ L/A cartridges
 Held using gauze
 Cervical (Ruddle 2002),or middle (Cohen
& Hargreaves 2006),exposed metal
surface
 Quickly move back & forth
Cold tests
 Begin with most posterior tooth
 Cotton pellet placed just distal to the
tooth
 Contact with adjacent gingiva or nearby
teeth: false responses
Cold tests
Refrigerant sprays
 Convenient & easiest to use
 Ranks just behind dry ice
 Dichlorodifluoromethane (DDM)
 Tetrafluoroethane (TFE)
 Propane butane mixture (PBM)
 -20oC to -50oC
Cold tests
 DDM: Freon-12
 Compressed spray: Endo-Ice (-50oC)
 DDM- production prohibited due to
environmental concerns
 Greater decrease in temperature than
dry ice & ethyl chloride
 Saturated cotton pellet:
 Multiple teeth : less effeicienty tested
Cold tests
 TFE: Green Endo-Ice (-26oC)
 No ozone depletion potenial
 Easy to use & rapid results
 Sprayed onto cotton pellet & applied to
middle third facial surface
 5s or until pain
 Equivalent to dry ice & even in restored
teeth
Cold tests
 PBM- Endo-Frost (-50oC)
 30-50% Propane, 30-50% butane & 30-50%
isobutane
 Nontoxic cold spray- freeze cotton pellets
& rolla
 Similar intrapulpal temperature decrease
Cold tests
Carbon di oxide snow/ Dry Ice
 Charles Thilorier -1835
 Dentistry: Back -1936
 Apparatus modified by Obwegser &
Steinhauser 1963: pencil like form
 -78oC; -56oC direct application
 Rapid response: <2 s
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Cold tests
Mechanism:
 PDJ temperature reduced to <2oC
 Hydrodynamic theory
 Enamel expansion / contraction & acts as
temperature transfer medium
(Linsuwanont et al 2007)
Cold tests
Technique
 CO2 released into special tube inside
plexiglass container: snow
 Compacted with a plugger: pencil/ stick
 Middle third of the facial surface of
crown: 2-5seconds or until pain
Cold tests
Advantages
 Accurate, reliable, consistent, fast &
uncomplicated
 1-2 minutes- without isolation
 Does not affect adjacent teeth
 Intense reproducible response
 Greater accuracy than EPT
Cold tests
 Full coverage restorations
 More reliable after trauma
 Under splinted abutments
 No false positive in necrosis
 Sustained lingering response: early
puplpitis
 Fixed orthodontic treatment
Cold tests
Disadvantages
 Not effective with calcified pulps
 More expensive than ethyl chloride/ ice
sticks
 More dependable results than ethyl
chloride/ ice (Fuss et al 1986, Andreasen
1976)
Cold tests
Ethyl chloride spray
 Chloroethane (-12.3oC)
 Colorless, flammable gas
 Skin refrigerant, mild topical anesthetic
 CNS depressant
 Better than EPTs & heated GP
 Not used: less effective than dry ice/ DDM
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Cold tests
Cold water bath
 Tooth/ group of teeth : isolated with
rubber dam
 Iced water syringed onto tooth
 Effective: simultaneous bathing of entire
crown
 Effective with full coverage restorations
 Better than ice sticks & no
armamentarium than rubber dam
 Time consuming
Heat tests
 Heat: fluid expansion- A fibers
 Inflamed pulp: C-fibers; lasting response
 Acutely inflamed/ partially necrotic pulp
 Low diagnostic accuracy- not used as
single method
Heat tests
Heated GP ( Grossman’s method)
 Warmed sticks of GP (120-140oC)
 Dry tooth surfaces & surrounding areas
with cotton rolls
 Iight coating of petroleum jelly
 GP stick warmed over flame
till glistening
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Heat tests
 Difficult to control temperature
 Concerns of damage to healthy pulp :
not with <5 s application
(Rickoff et al 1988)
 Reproducible results not obtained
 Lack of response in bulkier teeth
 Less consistent stimulus
 Limited value: posterior teeth & under
splints , temporary crowns
Heat tests
Warmed hand instruments
 Popular, not very reliable & poorly
assessed method
 Heated over a flame, held close to
buccal surface; without actually touching
 Not reproducible
 Difficult to control temperature & safety
problems
Heat tests
Electrical heat sources
 Touch ‘N Heat/ System B- 150oC
 Inserts: Hot Pup Test Tip
 Continuous heat mode- intensity set
 Tooth surface lubricated
Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
Castelucci. Endodontics Vol.1
Heat tests
Frictional heat
 Rubber cup- prophylaxis
 Buccal surface
 Best, easiest & safest
 Gold crown
 Seldom used today
Heat tests
Hot water bath
 Similar to cold water bath
 Temperature gradually increased
 Begin with most posterior and proceed
until positive response
 Greater thermal change
 PFM crowns
 Time consuming & patient cooperation
Remember..
 Inform patient of the nature of tests
 Hand signals
 Stimulus removed after 5-6 s
 Refractory period after cold test
 Cervical aspect (Petyers eta 1994, Ruddle
2002)
 middle third of buccal/ palatal aspect
(Cohen & Hargreaves 2006)
 Incisal- anterior & incisal aspect of
mesiobuccal cusp: posterior (Trope &
Debelian 2005)
 Ideally be tested on all surfaces
 Several adjacent, contralateral &
opposing teeth tested
 Individual perception
 Should not bias
Electrical pulp tests
 Direct stimulation of pulp nerve fibers
 Unreliable: necrotic & disintegrating pulp
tissue leaves electrolytes in pulp space
 Adequate stimulation, appropriate
technique, careful interpretation
 AC or DC; Pulsating DC: 5-15ms best
nerve stimulation
 Rate of current increase, strength duration
& frequency
Electrical pulp tests
 Benchtop style digital EPT
 Handheld style digital style EPT
 Handheld style analog EPT
EPT
 Monopolar/ Unipolar and Bipolar
 Mains power connection & Batteries
 Mid-1950’s: Bipolar- one electrode to the
other through tooth or one handheld
 Monopolar: anode on the lip & cathode
on the tooth
 Comparative studies: conflicting results
EPT & Histology
 No correlation between positive EPT &
histological status*
 Presence of sensory fibers that can
respond to electrical stimulus
 Quantification or comparison of
responses- not conclusive
 Cannot assess vitality
 Negative response- necrosis
Reynolds 1966, Mumford 1967b, Matthews et al 1974b, Cooley &
Robinson 1980
Technique of use
 Technique sensitive
 Removal of supragingival calculus
 Exterior surface dried & rubber dam
placed
 Insulation of proximal restorations
 Probe checked on skin- ensure current
flow
 Circuit completed
 Electrode coated with suitable medium
 Middle third of facial surface
 Direct contact necessary: small tip on
restored teeth
 Rheostat: 1-10, 1-15, 1-80
 Slowly increased: more accurate
 Procedure explained
 Tingling/ warm/stinging/ full/hot
 Shift tip position: if no response
 Tested 2 0r 3 times: ensure consistency
 Testing switched off / changing order;
eliminates bias & anxiety driven responses
 Full porcelain/ gold crowns
 Cavity prepared through restoration
without L/A until dentin
 If no response: EPT probe on dentin
 Rubber dam piece: insulate tip from
metal
 Highly different response: control tooth
Circuit completion
 Use without rubber gloves
 Lip clip: lose retentiveness & reliable
contact
 Touch the probe handle with finger: gives
patient control
 Modify EPT with metal rod
 Roll down dentist’s gloves: contact with
wrist & patient’s face
 Custom made patient held contact
device
 Stabilization groove cut on the probe
engaged by current conducting sleeve:
not recommended
Variations in reading/ False
response
Failure to complete
the circuit
 Equipment
problems
 Probe placement
 Interface media
Patient related factors
 Tooth characteristics
 Restored teeth
 Dentition
 Supporting tissues
 Apex maturation
 Repeated trials
 Psychological state
 Physiological state
False positive response
 Necrotic pulp responds to testing.
 Stimulation of adjacent teeth/
attachment apparatus
 The response of vital tissue in multirooted
tooth with pulp necrosis in one or more
canals
 Patient interpretation: subjectivity
William T. Johnson. Colour Atlas of Endodontics
False negative response
 Vital pulp that does not respond to
stimulation
 Inadequate contact with the stimulus
 Tooth calcification
 Immature apical development
 Traumatic injury
 Subjective nature of the tests
 Elderly patients – regressive neural changes
 Analgesics for pain
 Traumatic injury
Limitations of EPT
 No information on
health status/ integrity
 Unreliable for immature
teeth
 Not suitable with full
coverage restorations
 Chances of ventricular
fibrillation
Test cavity
 Non localized, acute diffuse radiating
pain
 Definitive diagnosis: impossible
 Cavity prepared in the tooth: concealed
position without anesthesia
 Patient apprised of what to expect & how
to respond
Test cavity
 Response: cavity preparation stopped &
restored again
 No response: endodontic access cavity
continued
 Low speed handpiece & small bur
recommended
 Full crown restorations & margins
contacting gingival tissue
Test cavity
 Young teeth: immature roots- invasive
nature questioned
 Unreliable; response even in necrotic pulp
 Response unreliable: anxiety
 Invasive & irreversible
 No further information than thermal & EPT
 Not justified in modern practice
Laser Doppler Flowmetry
Jafarzadeh .IEJ, 42, 476-
490,2009
 Optical measuring
method- number &
velocity of particles
conveyed by a
fluid flow to be
measured
 Laser light is
transmitted to the
pulp by means of a
fiber optic probe
Laser doppler
flowmetry
 Scattered light from the moving RBCs in
the circulation will be frequency-shifted,
while those from the static tissues remain
unshifted.
 Reflected light composed of Doppler
shifted and unshifted light is returned to
photodetectors
 Detected & processed -signal measure of
the blood flow in the dental pulp
Jafarzadeh .IEJ, 42, 476-490,2009
Laser doppler
flowmetry
 Not useful in teeth with crowns
and large restorations
 Detect only the coronal blood flow of the
pulp, which may not relate to the actual
blood flow on the linear scale.
Advantages:
 Painless diagnosis as compared to
thermal & electric pulp tests
 Diagnosis of immature or traumatized
teeth
Pulse Oximetry
 Effective, objective oxygen saturation
monitoring technique - intravenous
anesthesia
 Consistently determined the level of
blood oxygen saturation of the pulp- pulp
vitality testing
Jafarzadeh & Rosenberg. JOE Vol 35, No. 3, March 2009
Pulse Oximetry
 Correlation between pulp and systemic
oxygen saturation readings (Schnettler
and Wallace1991)- definitive pulp vitality
tester
 Biox 3740 Oximeter (Kahan et al 1996)
 Custom-made Pulse
Oximeter sensor holder
(Gopikrishna et al 2006)
Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
Pulse Oximetry
 Probe containing two LEDs: red light-
660 nm & infrared light (900–940 nm)
 Measures absorption of oxygenated and
deoxygenated Hb
 Received by a photodetector diode
connected to a microprocessor.
 Relationship between the pulsatile change in
the absorption of red light & infrared light :
assessed by the oximeter + known absorption
curves for oxygenated and deoxygenated
hemoglobin,
Jafarzadeh & Rosenberg. JOE Vol 35, No. 3, March 2009
Pulse Oximetry
Indications:
 Recent trauma
 Primary &
immature
permanent teeth
 Patient monitoring:
sedation
Limitations:
 Intrinsic interference:
venous blood &
tissue constituents,
acidity,CO2
 Extrinsic interference
 Well adapting sensor
 Hb bound to other
gases
 Extensive restorations
Jafarzadeh & Rosenberg. JOE Vol 35, No. 3, March 2009
Pulse Oximetry
 70%- 100% accuracy
 Inverse correlation between saturation
values & EPT readings (Radhakrishnan et
al 2002)
 More sensitive & specific compared to
cold tests & EPT (Gopikrishna et al 2007)
Jafarzadeh & Rosenberg. JOE Vol 35, No. 3, March 2009
Dual Wavelength
Spectrophotometry
 Method independent of a pulsatile
circulation
 Measures oxygenation changes in the
capillary bed rather than in the supply
vessels
 Detects the presence or absence of
oxygenated blood at 760 nm and 850nm.
 Advantage: Uses visible light that is filtered
and guided to the tooth by fibreoptics
Divya et al.Contemporary Diagnostic AIDS in Endodontics”. Journal of Evolution of Medical
and Dental Sciences 2014; Vol. 3, Issue 06, February 10
Ultraviolet light/Fiberoptic
Fluorescent Spectrometry
 Fluorescence
 Vital teeth fluoresce normally; necrotic &
RCT teeth do not –Foreman
 Lighting in the operatory fully suppressed
 Patient & staff wear suitable protective
goggles
 Fluorescence from the pulp -substantially
lower than the healthy and decayed
dentin fluorescence.
 Healthy and decayed dentin patterns
differentiated
Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
Photoplethysmography
 Optical measurement technique : blood
volume changes in the microvascular bed
of tissue.
 Light source to illuminate the tissue & a
photodetector to measure the small
variations in light intensity associated with
changes in perfusion
Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
Anesthetic test
 L/A: painful area
 Block/ infiltration/ intraosseous
 Vague location of pain
 Non odontogenic pain:Myocardial
infarction
 Differentiating between arches
 PDL- identify source of pulpal pain.
 Dentin sterilizing : Silver nitrate, phenol,
eugenol & desensitizing substances
 Cleansers: Alcohol, chloroform, H2O2,
various acids
 Restorative materials & liners
Besner, Ferrigno. Practical Endodontics- A Clinical Guide
Tooth surface temperature
 Fanibunda: pulp circulation maintains
tooth temperature
 Cholesteric crystals- 10% solution in
chlorinated hydrocarbon solvent(Howell
et al)- non vital: lower temperature
 Thermistor: vital & RCT teeth- with and
without gold crowns (Banes & Hammond)
 Consistent (Stoops & Scott)
Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
Tooth surface temperature
 Electronic thermography: Infrared sensor,
control unit, thermal image computer,
software, color monitor, printer
 Differences in deep & superficial areas-
not sensitive
 Hughes Probeye 4300 thermal video
system: sensitive to measure 0.1oc
 Adjunct to other diagnostic tests
Patient temperature
 Baseline temperature: follwed up
 Patient is improving/ worsening
 >1000oF : systemic response to infection
Ultrasound
 Compliment conventional radiography
 High resolution, 3D images- inner
macrostructure of the tooth
 A transducer (a crystal containing probe),
a coupling agent & software
 Detect cracks in a simulated human tooth
 Detect vertical root fractures – vital &
nonvital teeth
Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
Ultrasonic Doppler Imaging
 Blood circulation detected
 Distinguish vital teeth from root- filled
teeth: blood flow parameters, waveform,
sound
 Promising tool- traumatically injured teeth
 Power Doppler associated with color
Doppler – improved sensitivity to low flow
rates
Yoon et al. JOE- Volume 36, No.3, March 2010
Vital tooth
Non vital tooth
Optical Reflection Vitalometry
 Preliminary report-1997 (Oikarinen et al)
 Noninvasive method
 The pulse of the pulp/oral mucosa.
 Yet to be clinically accepted &
commercially available.
Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
Evaluation of Sensibility Tests
 Thermal test: Endo Ice & EPT- evaluated
 Endo Ice- 0.904 accuracy & EPT- 0.75
 Age group 21-50 & vital teeth: more
accurate response to cold test

Jespersen et al. JOE- Volume 40, No.3, March 2014
RADIOGRAPHY-Little value : assess
pulp status
 Presence & extent
of carious lesions
 Vital pulp therapy
 Calcifications
 Resorptions
 Periradicular
radiolucencies
 Tracing fistulous
tracts
 Thickness of PDL
 Periodontal
disease
 Root & pulp space
anatomy
 Previous RCT
 Bitewing: pulp
chamber
 Eccentric ray
alignment
Beer, Bauman, Kim. Color Atlas of
Endodontology
Digital radiography
 Variables in diagnostic quality of
conventional radiography- controlled
 Image- enhanced,
colorized and useful
patient education tool
Cone Beam Volumetric
Tomography
 First used in
dentistry- Mozzo P
et al 1998
 Proximity to
anatomic
structures
 Root canal
anatomy
Diagnosis: never based solely on radiographic
finding
Thank you!!!!
References
 Endodontic therapy – Weine
 Endodntics6- Ingle et al
 Cohen’s sPathways of the Pulp- 10th ed
 Color Atlas of Endodontics- William T.
Johnson
 Endodontics- Problem solving in Clinical
practice- Pitt Ford
 Practical Endodontics- A clinical guide.
Bessner & Ferrigno
 Pocket Atlas of Endodontics- Beer
 H. Jafarzadeh & P. V. Abbott. Review of
pulp sensibility tests. Part I: general
information and thermal tests. IEJ, 43, 738-
762, 2010
 Yoon et al. JOE- Volume 36, No.3, March
2010
 Jespersen et al. JOE- Volume 40, No.3,
March 2014

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Endodontic diagnosis

  • 1. ENDODONTIC DIAGNOSIS Deepthi P.R. 1st year MDS Dept of Conservative Dentistry & Endodontics
  • 2. CONTENTS  Introduction  Diagnosis  Diagnostic method  Medical history  Drugs & medication history  Dental history  Subjective symptoms  Clinical observations  Clinical tests
  • 3. Introduction  Thorough knowledge of other sciences  Diagnosis & Treatment planning  Pain of non odontogenic origin  Accurate database:  Medical & dental history  Clinical examination & relevant tests  Making & interpreting appropriate radiographs
  • 4. Diagnosis  ‘The art and science of detecting deviations from health and the cause and nature thereof’  Differential diagnosis: ‘The process of identifying a condition by comparing the symptoms of all (or other) pathologic process that may produce signs and symptoms ’ Glossary of endodontic terms. 7th ed. Chicago: American Association of Endodontists;2003
  • 5. Diagnosis  Inability to test/ image the tissue directly  Indirect interpretation of response to stimuli  Determine teeth free of disease rather than diseased Newton et al. JOE- Volume 35, Number 12, December 2009
  • 6. Diagnostic method METHODS Pulp testing Palpation Percussion DIAGNOSTIC APPROACHES Bite test Test cavity Staining/ Transillumination Selective anesthesia Radiography Dental history/ Medical history Evaluation of pain signs/ symptoms Newton et al. JOE- Volume 35, Number 12, December 2009
  • 7. Surgical Sieve Pitt Ford & Rhodes. Endodontics- Problem solving in Clinical Practice • Biographical details • Medical history • Chief complaint • History of present complaint • Dental history • Social history • Extraoral examination • Intraoral examination • Special tests • Radiographs • Diagnosis • Treatment plan
  • 8. s A sample form used in diagnosis and treatment planning. (Adapted from Krell K, Walton R: Odontalgia: diagnosing pulpal, periapical, and periodontal pain. In Clark J, editor: Clinical dentistry, Philadelphia, 1987, Harper & Row.)
  • 9. Medical history  Treatment: harmonious with general health  Impact of the patient’s health on the dental operating team  Alterations in the usual course of treatment  Name & contact of physician
  • 10. Rheumatic fever Potential for SBE after bacteremia Antibiotic premedication:  Artificial heart valves: Same antibiotic coverage: rheumatic fever Pulp extirpation Filing beyond the apex Rubber dam placement Initial appointment/ Surgical appointment Possibilty of going past the apex Periapical lesion
  • 11. Coronary Artery disease  Physician consultation: anticoagulant  Non surgical treatment preferred  Mild / moderate analgesics  Brief recess: more than one tooth- single appointment  Substernal pain: dressing placed & treatment terminated; referred to physician
  • 12. Hypertension  Injection of L/A solutions < 30sec/ml  Warm anesthetic solutions: few minutes before injection  Tranquil mood created- minimal mention of complications & failures  Hypnotic premedication: consultation with physician
  • 13. Hypertension  Avoid G/A & no more than 3 anesthetic carpules  Morning appointments preferred  Night time premedication with early appointments  Total appointment time not > 1 hour  Terminate when patient is stressed
  • 14. Diabetes  Retarded healing: postop radiographs  Antibiotics: Infection/ surgery 1yearPre op 6 months 2 years1.5 years 3 years
  • 15. Diabetes  Alteration in blood glucose levels: physician consultation  Epinephrine avoided: Increase in blood glucose levels & tissue sloughs post surgery  Levonordefrin  Barbiturates & sedatives cautiously used
  • 16. Diabetes  Longer & deeper anesthesia  L/A preferred  Appointments: soon after meals  Differentiate & manage hypoglycemia/ hypoinsulinism • Mepivacaine +Levonordefrin • Propoxyphene+ Procaine +Levarterenol bitartarate
  • 17. Hepatitis  Resistant to normal sterilization  Intracanal instruments: discarded after use  Avoid drugs detoxified in the liver: Halothane,Erythromycin  Cautious- Paracetamol
  • 18. Blood diseases  Internal bleeding: L/A administration  Avoid injections: necrotic pulp  Vital pulp: First appt. •Access to the cavity •Dressing Second appt •A week later •Fixed pulpal tissue removed •Dressing replaced Process continued: vital tissue removed Canals enlarged & filled
  • 19. Blood diseases  Rubber dam: Notches- labial & lingual surfaces  Gingival bleeding: do not treatment without systemic diagnosis  Infectious mononucleosis:  Avoided in acute stage • Pain • Exacerbations • Exaggerated response to drugs
  • 20. Joint replacement prostheses  Bacteremia  Antibiotic coverage  Painful joint after procedure: orthopedic surgeon consulted  Longer than usual: desirable results  Hypersensitivity states: drugs only when absolutely indicated  Avoid new/ unusual drugs  HIV: transmission avoided- proper asepsis Other serious Diseases
  • 21. Recent change in weight Weight loss  Dieting  Loss of appetite  Systemic diseases Weight gain  Psychogenic reasons  Hormonal disturbances  Pregnancy  Protect exposed tooth surfaces after endodontic therapy  Salt & water retention
  • 22. Psychologic problems  Physical problems: tendency towards anxiety  Patients on Tranquilizers/ antidepressants Converted a psychologic condition to physical problem Severe fears & anxieties – treatment difficult • No relief with treatment • Pulpal problem suspected: suspicious oral conditions • Friendly and firm • Instruments: out of sight • Informative booklets • Smooth & painless initial visit
  • 23. Others Hyperthyrodism  No epinephrine  Increase sedative if needed Ulcers  Avoid aspirin & if on antacids- avoid tetracycline  Use Penicillin V if needed Alcoholic  Cautious with sedatives  Aspirin avoided
  • 24. Drugs & Medication therapy  Physical condition & effects of medications  Adverse reactions  Questionnaire format  Unaware of Drug’s contents : Mosby’s Drug Consult/ physician  History of allergy: minimum inter appointment time & well monitored
  • 25. Drugs & Medication therapy  Steroid therapy: intratreatment pain & exacerbations , infections  Appointments: maximum 3 days apart  Vital: 2 sitting & Necrotic: 3 sitting – 1 week period  Surgery- Antibiotic therapy & steroid dose
  • 26. Drugs & Medication therapy  Aspirin: bleeding after surgery  Avoid- Blood dyscrasia, anticoagulant, renal transplant, gout  Caution- Asthma, Diabetes, Last month of pregnancy  Tranquilizer therapy: unusual reactions to prescribed hypnotics/ narcotics  Physician consulted
  • 27. Drugs & Medication therapy • CNS stimulant: increase sedative dose • Sulfonamides: avoid procaine Antidepressants: Cautious • GA • Narcotics • Antisialagogue Tetracycline: • Antacids • Penicillin Barbiturates : cautious • Dilantin • Griseofulvin • Steroids
  • 28. Dental history  Patient’s objective for treatment- clear  Appreciation for dental treatment  Experiences with previous dentist Pain relief Check up Oral systemic relation CosmeticsMasticatory inefficiency
  • 29. Dental history  Chief complaint & its history  When was it last restored?  Pulp capping/ Pulpotomy/ large restoration in the same  Sharp blow/ accident  Swelling/ gum boil  Drainage
  • 30. Subjective symptoms  Is the pain still present?  What type? (Sharp/ dull)  Throbbing?  Intermittent/ Continuous?  Aggravated by: cold, heat, pressure, mastication, lying down, sweet, sour?  How long does it last?
  • 31. Clinical Observations  Extraoral swelling  Lymph node involvement  Intraoral involvement  Fistula  Tooth discoloration  Traumatic injuries: fractures  Deep carious lesion  Recurrent caries beneath a restoration  Extensive restoration  Developmental defects of teeth  Gingival recession
  • 32. Clinical Tests Diagnostic tests: 1. EPT 2. Thermal tests 3. Percussion 4. Palpation 5. Mobility 6. Periodontal evaluation 7. Occlusal evaluation 8. Radiograph Selective tests for Difficult Diagnostic Situations: 9. Test cavity preparation 10. Anesthetic test 11. Transillumination 12. Biting 13.Staining 14. Gutta percha point tracing with radiograph
  • 33. Extraoral examination  External facial form & features  Fistulae, erythema, pallor  Neurologic examination: motor function, sensitivity, movement  Lymph nodes: inflammatory, infectious, tumor like disorders
  • 34. Intraoral examination Soft tissue examination:  Swelling/ fistula
  • 35. Intraoral examination  Crown discoloration: non vital pulp, removal of discolored dentin, use of chlorinated soda  Deep carious lesions/ fractures: visual examination & probing
  • 36. Percussion test  Simple, but useful  Inflammatory condition of the apical periodontium  First clinical indications of apical periodontitis
  • 37. Percussion test  Symptomatic apical periodontitis: more sensitive  Pulpal diseases: not reveled unless apical periodontium is involved  Periodontal/ endodontic etiology, occlusal trauma, combination with marginal periodontitis  Horizontal percussion
  • 38. Percussion test  Firm digital pressure/ handle of instrument like mouth mirror: tap in a vertical direction  Patient bite on Tooth Slooth/ Cotton swab  Several teeth repeatedly  Random order
  • 39. Palpation  Vestibular region: apical region of the root tips  Tenderness, swelling, fluctuation, hardness, crepitation  Tip of index finger  Usefulness increase with skill & clinical experience
  • 40. Mobility  Moving in a buccal- lingual direction  Index finger on the lingual surface & lateral force applied with instrument handle from buccal surface  Using two fingers
  • 41. Mobility Miller’s index:  Class 1- First distinguishable sign of greater- than- normal movement  Class 2- Movement of the crown as much as 1mm in any direction  Class 3- Movement of the crown more than 1 mm in any direction and/or vertical depression/ rotation of the crown in its socket
  • 42. Periodontal probing  Endodontic & periodontic lesions mimic each other concurrently  Record probing depths: periodontal health & prognosis  Entire circumference probed
  • 43. Periodontal probing Narrow isolated probing defects:  Periodontal disease  Sinus- like trap following periapical pathosis  Vertical groove defect  Cracked teeth  Vertical root fractures  External root resorption
  • 44. Tests for Cracked Tooth Syndrome Transillumination  Fiberoptic light  Coronal cracks/ vertical root fractures  Minimal background lighting  Light placed on varied surfaces of coronal tooth structure/ root after flap refection
  • 45. Transillumination  Light traverses fracture lines- visually detected  Fractured Segment near the light appears brighter
  • 46. Dye staining  Dye penetrates fracture line  Demonstrates fractures  Apply – internal surfaces of cavity preparation/ access opening  Leave it in place for a week  Iodine/ methylene blue dye
  • 47. Dye staining 3 methods: Remove restoration:  Direct revealing of fracture line  Dye incorporated into ZOE mixture & placed  Patient chews on disclosing tablet Bessner & Ferrigno. Practical guide to Endodontics
  • 48. Bite test  Wooden stick- opposing teeth  Tooth slooth  Patient bites down & pain elicited upon release  Rubber dam sheet- cracked cusp flexes
  • 49. Pulp tests  Major & essential part of diagnostic process  Reproduce patients symptoms, diagnose diseased tooth & disease  2 independent diagnostic test results Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 50. Pulp tests  *Ideal technique: non invasive, painless, standardized, reproducible, reliable, inexpensive, easily completed & objective *Chambers. 1982 Pulp sensibility tests • Thermal tests • Electric pup tests • Test cavity Pulp vitality tests • Laser doppler flowmetry • Pulse oximetry • Tooth temperature measurement
  • 51. Pulp sensibility tests  Pulp nerve fibers respond – external stimulus  Thermal/ Electrical / Direct dentine stimulation  Do not indicate the health status & unreliable responses Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 52. Pulp sensibility tests  No indication of vitality: intact vasculature  Correlation between test results & necrotic pulps only*  Assess whether necrotic or not & does not quantify the degree of disease  Useful : identifying diseased tooth *Seltzer et al.1963, Tyldesley & Mumford 1970, Dummer et al, 1980
  • 53. Pulp sensibility tests Preferred sequence:  Tests repeated after 1’ recovery time  Thermal tests: no method to assess how responsive the tooth is or to compare with previous result  EPT: numerical display- not essentially reproducible Disease free contralateral teeth Opposing teeth Presumably healthy teeth- same quadrant Most suspicious tooth
  • 54. Rationale of the tests  Sharp, non lingering pain- application of thermal stimulation: normal  A - 25% stimulus required to activate C fibers* *Virtanen 1985, Hargreaves & Goodis 2002
  • 55. Thermal tests- Rationale  Sensory response: not by temperature changes in receptors  Hydrodynamic movement of fluid: dentinal tubules- A fibers  Cold- faster A fibers: sharp localized pain  Heat- slower C fibers: dull long lasting pain Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 56. Electric Pulp Test - Rationale  Current sufficient to overcome the resistance of enamel & dentine- stimulate A fibers  Sensation felt with gradually increasing level of current: pulp responsive/ partially alive  *Ionic shift in tubules local depolarization action potential Pantera et al. 1993
  • 57. EPT- Rationale  A fibers: brief sharp sensation/ tingling  *No blood flow- pulp becomes anoxic & A fibers cease to function *Pitt Ford & Patel 2004
  • 58. Indications 1.Pain in the trigeminal area; referred pain 2. Periodical monitoring of teeth after trauma  1-8 weeks lapse before normal response  EPT: reliable after trauma** *No response Response : Recovery Repetitious response :Healthy pulp Response No response: Degeneration No response persistent: Necrotic pulp **Ingle et al 2002,*Bhaskar & Rappaport 1973
  • 59. Indications 3. Assessment of pulpal health before restorative procedures  potential prosthetic abutment 4. Pulp preservation procedures & extensive restorations 5. Differentiate periapical radiolucencies from normal anatomical structures & non odontogenic lesions Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 60. Indications 6. Predict potential anesthetic problems & evaluation of analgesics  Cold test: assess pulpal anesthesia  Preoperative pulp-test performed  Traditional parameters verified  Retested with the same test  Prepared for treatment & level of anesthesia screened Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 61. 7. Pulp status of transplanted teeth
  • 62. Indications 8. Le Fort type fractures/ osteotomies  Normal: 7-11 months after surgery
  • 63. Limitations 1. Subjective; measure only nerve supply 2. Thermal tests: not effective in substantial secondary dentine formation 3. Unreliability of tests: Immature apices, traumatic injuries, more subjectivity in the young 4. No correlation with the histologic status (Contrasting results: Hill, 1986) Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 64. Limitations 5. Difficult to administer & inconclusive in children 6. Weaker response- aged pulp 7. Extensive restorations, pulp recession, pulp calcification 8. Lack of reproducibility Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 65. Interpretation- Diagnosis  Immediacy, intensity & duration of response  Outcome: never certain  No particular response- unique to specific pathologic states Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 66. Clinically Normal pulp  Mild to moderate transient response to cold & electrical stimuli  Response subsides in few seconds on removal of stimulus  Do not usually respond to heat tests
  • 67. Reversible pulpitis  Thermal stimuli (cold)- sharp pain  Subsides as soon as the stimulus is removed/ in few seconds
  • 68. Irreversible pulpitis  Thermal changes (cold): sharp pain , dull prolonged ache- last upto an hour or so  Valuable: stimulus as reported by patient applied & pain reproduced & assessed  EPT: not of value
  • 69. Pulp necrosis  Histological state not determined  Significant relation between lack of response & pulp necrosis  No response with EPTs & thermal tests  No indication of infection expected from these
  • 70. Pulp necrobiosis  Difficult to diagnose  History : pulpitis  Pulp tests: necrosis  Vague response to EPTs, cold tests
  • 71. Periapical conditions Acute apical periodontitis  Maybe associated with pulpitis  Pulp status assessed before treatment Acute apical abscess  Negative Lateral periodontal abscess  Positive Chronic apical periodontitis  Sequel of infected canal system
  • 72. False responses False negative results: Normal pulps that do not respond to tests  Calcification: no response to cold; may respond to high value of current in EPT  Premedication  Recent trauma  Immature apex  RCT teeth: not expected to respond Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 73. False responses  Extensive restorations  Pulp protecting bases  High pain threshold  Activation of fixed orthodontic appliances  Psychotic disorders  Defective EPT device/ discharged batteries/ poor electrical contact
  • 74. False responses False positive results: Necrotic pulps responding to tests  Conduction of current to adjacent gingival & periodontal tissues (avoided with reasonable current strength & proper techniques)  Moist gangrene, partially necrotic tissue, infected pulp  Breakdown products of localized necrosis
  • 75. False responses  Calcified tooth structure conducting to tissue apical to an area of necrosis  Current conducted to adjacent teeth through metallic restorations (avoided by rubber dam / celluloid strips between teeth)  Inflamed pulp tissue in one canal of a multirooted teeth with other canals & chamber necrotic  Anxious/ young patient
  • 76. False responses  More common with EPT than cold test  EPTs: all teeth; cold tests: multirooted teeth  EPT: rare false negative, if more than one surface used  Cold test: sometimes, only cervical area responds
  • 77. Value of diagnostic tests  Precision: ‘Tendency of repeated measurements on the same sample to yield the same result’  Variability: Lack of precision  Accuracy: The extent to which a test correctly classifies patient’s response  Sensitivity: The ability of the test to detect the disease in patients who actually have the disease Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 78. Value of diagnostic tests  Specificity: The ability of a test to detect the absence of a result  Positive predictive value: The probability that a positive test result actually represents a disease positive tooth  Negative predictive value: The probability that a tooth with a negative test result is actually free from the disease
  • 79. Value of diagnostic tests  Heat: relatively high sensibility; but least accurate being the least specific  Cold test: more accurate than EPT
  • 80. Thermal tests  Often inappropriately referred to as ‘Vitality tests’  More reliable than EPT  Inexpensive & easy-to- use equipment  Patient’s pain reproduced
  • 81. Thermal tests • Initial cold sensitivity • Heat sensitivity- continued pulp deterioration • Disappearance of cold sensitivity • Cold stimuli might relieve heat induced pain
  • 82. Damage to hard & soft tissues of the tooth  Heat test: more potential to injure  Tissue freezing: -100c for 5-20’  Intracellular ice crystal formation & ischemic necrosis following vascular injuries  -220c lowered pulp temperature to 110c: caused no damage (Langeland et al, 1969)
  • 83. Damage to hard & soft tissues of the tooth  Conflicting reports: Dry ice inducing enamel cracks  Delayed cold transfer process: Cold stimulus applied to necrotic pulps under a bridge- felt by adjacent tooth  ‘Film boiling’/ ‘ Leidenfrost phenomenon’: Insulating layer of CO2 gas around dry ice, if it falls into mouth
  • 84. Cold tests Ice sticks  0oC temperature  Not accurate: adult posterior teeth  Secondary/ reparative dentin deposition  Testing under crowns/ splints  Application- 5s : reliable & valid  Disadvantage: less effective stimulation Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 85. Cold tests  Freezing water- hypodermic needles’ plastic cover/ L/A cartridges  Held using gauze  Cervical (Ruddle 2002),or middle (Cohen & Hargreaves 2006),exposed metal surface  Quickly move back & forth
  • 86. Cold tests  Begin with most posterior tooth  Cotton pellet placed just distal to the tooth  Contact with adjacent gingiva or nearby teeth: false responses
  • 87. Cold tests Refrigerant sprays  Convenient & easiest to use  Ranks just behind dry ice  Dichlorodifluoromethane (DDM)  Tetrafluoroethane (TFE)  Propane butane mixture (PBM)  -20oC to -50oC
  • 88. Cold tests  DDM: Freon-12  Compressed spray: Endo-Ice (-50oC)  DDM- production prohibited due to environmental concerns  Greater decrease in temperature than dry ice & ethyl chloride  Saturated cotton pellet:  Multiple teeth : less effeicienty tested
  • 89. Cold tests  TFE: Green Endo-Ice (-26oC)  No ozone depletion potenial  Easy to use & rapid results  Sprayed onto cotton pellet & applied to middle third facial surface  5s or until pain  Equivalent to dry ice & even in restored teeth
  • 90. Cold tests  PBM- Endo-Frost (-50oC)  30-50% Propane, 30-50% butane & 30-50% isobutane  Nontoxic cold spray- freeze cotton pellets & rolla  Similar intrapulpal temperature decrease
  • 91. Cold tests Carbon di oxide snow/ Dry Ice  Charles Thilorier -1835  Dentistry: Back -1936  Apparatus modified by Obwegser & Steinhauser 1963: pencil like form  -78oC; -56oC direct application  Rapid response: <2 s Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 92. Cold tests Mechanism:  PDJ temperature reduced to <2oC  Hydrodynamic theory  Enamel expansion / contraction & acts as temperature transfer medium (Linsuwanont et al 2007)
  • 93. Cold tests Technique  CO2 released into special tube inside plexiglass container: snow  Compacted with a plugger: pencil/ stick  Middle third of the facial surface of crown: 2-5seconds or until pain
  • 94. Cold tests Advantages  Accurate, reliable, consistent, fast & uncomplicated  1-2 minutes- without isolation  Does not affect adjacent teeth  Intense reproducible response  Greater accuracy than EPT
  • 95. Cold tests  Full coverage restorations  More reliable after trauma  Under splinted abutments  No false positive in necrosis  Sustained lingering response: early puplpitis  Fixed orthodontic treatment
  • 96. Cold tests Disadvantages  Not effective with calcified pulps  More expensive than ethyl chloride/ ice sticks  More dependable results than ethyl chloride/ ice (Fuss et al 1986, Andreasen 1976)
  • 97. Cold tests Ethyl chloride spray  Chloroethane (-12.3oC)  Colorless, flammable gas  Skin refrigerant, mild topical anesthetic  CNS depressant  Better than EPTs & heated GP  Not used: less effective than dry ice/ DDM Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 98. Cold tests Cold water bath  Tooth/ group of teeth : isolated with rubber dam  Iced water syringed onto tooth  Effective: simultaneous bathing of entire crown  Effective with full coverage restorations  Better than ice sticks & no armamentarium than rubber dam  Time consuming
  • 99. Heat tests  Heat: fluid expansion- A fibers  Inflamed pulp: C-fibers; lasting response  Acutely inflamed/ partially necrotic pulp  Low diagnostic accuracy- not used as single method
  • 100. Heat tests Heated GP ( Grossman’s method)  Warmed sticks of GP (120-140oC)  Dry tooth surfaces & surrounding areas with cotton rolls  Iight coating of petroleum jelly  GP stick warmed over flame till glistening Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010
  • 101. Heat tests  Difficult to control temperature  Concerns of damage to healthy pulp : not with <5 s application (Rickoff et al 1988)  Reproducible results not obtained  Lack of response in bulkier teeth  Less consistent stimulus  Limited value: posterior teeth & under splints , temporary crowns
  • 102. Heat tests Warmed hand instruments  Popular, not very reliable & poorly assessed method  Heated over a flame, held close to buccal surface; without actually touching  Not reproducible  Difficult to control temperature & safety problems
  • 103. Heat tests Electrical heat sources  Touch ‘N Heat/ System B- 150oC  Inserts: Hot Pup Test Tip  Continuous heat mode- intensity set  Tooth surface lubricated Jafarzadeh & Abbott. IEJ, 43, 738-762, 2010 Castelucci. Endodontics Vol.1
  • 104. Heat tests Frictional heat  Rubber cup- prophylaxis  Buccal surface  Best, easiest & safest  Gold crown  Seldom used today
  • 105. Heat tests Hot water bath  Similar to cold water bath  Temperature gradually increased  Begin with most posterior and proceed until positive response  Greater thermal change  PFM crowns  Time consuming & patient cooperation
  • 106. Remember..  Inform patient of the nature of tests  Hand signals  Stimulus removed after 5-6 s  Refractory period after cold test  Cervical aspect (Petyers eta 1994, Ruddle 2002)  middle third of buccal/ palatal aspect (Cohen & Hargreaves 2006)
  • 107.  Incisal- anterior & incisal aspect of mesiobuccal cusp: posterior (Trope & Debelian 2005)  Ideally be tested on all surfaces  Several adjacent, contralateral & opposing teeth tested  Individual perception  Should not bias
  • 108. Electrical pulp tests  Direct stimulation of pulp nerve fibers  Unreliable: necrotic & disintegrating pulp tissue leaves electrolytes in pulp space  Adequate stimulation, appropriate technique, careful interpretation  AC or DC; Pulsating DC: 5-15ms best nerve stimulation  Rate of current increase, strength duration & frequency
  • 109. Electrical pulp tests  Benchtop style digital EPT  Handheld style digital style EPT  Handheld style analog EPT
  • 110. EPT  Monopolar/ Unipolar and Bipolar  Mains power connection & Batteries  Mid-1950’s: Bipolar- one electrode to the other through tooth or one handheld  Monopolar: anode on the lip & cathode on the tooth  Comparative studies: conflicting results
  • 111. EPT & Histology  No correlation between positive EPT & histological status*  Presence of sensory fibers that can respond to electrical stimulus  Quantification or comparison of responses- not conclusive  Cannot assess vitality  Negative response- necrosis Reynolds 1966, Mumford 1967b, Matthews et al 1974b, Cooley & Robinson 1980
  • 112. Technique of use  Technique sensitive  Removal of supragingival calculus  Exterior surface dried & rubber dam placed  Insulation of proximal restorations  Probe checked on skin- ensure current flow
  • 113.  Circuit completed  Electrode coated with suitable medium  Middle third of facial surface  Direct contact necessary: small tip on restored teeth  Rheostat: 1-10, 1-15, 1-80  Slowly increased: more accurate
  • 114.  Procedure explained  Tingling/ warm/stinging/ full/hot  Shift tip position: if no response  Tested 2 0r 3 times: ensure consistency  Testing switched off / changing order; eliminates bias & anxiety driven responses
  • 115.  Full porcelain/ gold crowns  Cavity prepared through restoration without L/A until dentin  If no response: EPT probe on dentin  Rubber dam piece: insulate tip from metal  Highly different response: control tooth
  • 116. Circuit completion  Use without rubber gloves  Lip clip: lose retentiveness & reliable contact  Touch the probe handle with finger: gives patient control  Modify EPT with metal rod
  • 117.  Roll down dentist’s gloves: contact with wrist & patient’s face  Custom made patient held contact device  Stabilization groove cut on the probe engaged by current conducting sleeve: not recommended
  • 118. Variations in reading/ False response Failure to complete the circuit  Equipment problems  Probe placement  Interface media Patient related factors  Tooth characteristics  Restored teeth  Dentition  Supporting tissues  Apex maturation  Repeated trials  Psychological state  Physiological state
  • 119. False positive response  Necrotic pulp responds to testing.  Stimulation of adjacent teeth/ attachment apparatus  The response of vital tissue in multirooted tooth with pulp necrosis in one or more canals  Patient interpretation: subjectivity William T. Johnson. Colour Atlas of Endodontics
  • 120. False negative response  Vital pulp that does not respond to stimulation  Inadequate contact with the stimulus  Tooth calcification  Immature apical development  Traumatic injury  Subjective nature of the tests  Elderly patients – regressive neural changes  Analgesics for pain  Traumatic injury
  • 121. Limitations of EPT  No information on health status/ integrity  Unreliable for immature teeth  Not suitable with full coverage restorations  Chances of ventricular fibrillation
  • 122. Test cavity  Non localized, acute diffuse radiating pain  Definitive diagnosis: impossible  Cavity prepared in the tooth: concealed position without anesthesia  Patient apprised of what to expect & how to respond
  • 123. Test cavity  Response: cavity preparation stopped & restored again  No response: endodontic access cavity continued  Low speed handpiece & small bur recommended  Full crown restorations & margins contacting gingival tissue
  • 124. Test cavity  Young teeth: immature roots- invasive nature questioned  Unreliable; response even in necrotic pulp  Response unreliable: anxiety  Invasive & irreversible  No further information than thermal & EPT  Not justified in modern practice
  • 125. Laser Doppler Flowmetry Jafarzadeh .IEJ, 42, 476- 490,2009  Optical measuring method- number & velocity of particles conveyed by a fluid flow to be measured  Laser light is transmitted to the pulp by means of a fiber optic probe
  • 126. Laser doppler flowmetry  Scattered light from the moving RBCs in the circulation will be frequency-shifted, while those from the static tissues remain unshifted.  Reflected light composed of Doppler shifted and unshifted light is returned to photodetectors  Detected & processed -signal measure of the blood flow in the dental pulp Jafarzadeh .IEJ, 42, 476-490,2009
  • 127. Laser doppler flowmetry  Not useful in teeth with crowns and large restorations  Detect only the coronal blood flow of the pulp, which may not relate to the actual blood flow on the linear scale. Advantages:  Painless diagnosis as compared to thermal & electric pulp tests  Diagnosis of immature or traumatized teeth
  • 128. Pulse Oximetry  Effective, objective oxygen saturation monitoring technique - intravenous anesthesia  Consistently determined the level of blood oxygen saturation of the pulp- pulp vitality testing Jafarzadeh & Rosenberg. JOE Vol 35, No. 3, March 2009
  • 129. Pulse Oximetry  Correlation between pulp and systemic oxygen saturation readings (Schnettler and Wallace1991)- definitive pulp vitality tester  Biox 3740 Oximeter (Kahan et al 1996)  Custom-made Pulse Oximeter sensor holder (Gopikrishna et al 2006) Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
  • 130. Pulse Oximetry  Probe containing two LEDs: red light- 660 nm & infrared light (900–940 nm)  Measures absorption of oxygenated and deoxygenated Hb  Received by a photodetector diode connected to a microprocessor.  Relationship between the pulsatile change in the absorption of red light & infrared light : assessed by the oximeter + known absorption curves for oxygenated and deoxygenated hemoglobin, Jafarzadeh & Rosenberg. JOE Vol 35, No. 3, March 2009
  • 131. Pulse Oximetry Indications:  Recent trauma  Primary & immature permanent teeth  Patient monitoring: sedation Limitations:  Intrinsic interference: venous blood & tissue constituents, acidity,CO2  Extrinsic interference  Well adapting sensor  Hb bound to other gases  Extensive restorations Jafarzadeh & Rosenberg. JOE Vol 35, No. 3, March 2009
  • 132. Pulse Oximetry  70%- 100% accuracy  Inverse correlation between saturation values & EPT readings (Radhakrishnan et al 2002)  More sensitive & specific compared to cold tests & EPT (Gopikrishna et al 2007) Jafarzadeh & Rosenberg. JOE Vol 35, No. 3, March 2009
  • 133. Dual Wavelength Spectrophotometry  Method independent of a pulsatile circulation  Measures oxygenation changes in the capillary bed rather than in the supply vessels  Detects the presence or absence of oxygenated blood at 760 nm and 850nm.  Advantage: Uses visible light that is filtered and guided to the tooth by fibreoptics Divya et al.Contemporary Diagnostic AIDS in Endodontics”. Journal of Evolution of Medical and Dental Sciences 2014; Vol. 3, Issue 06, February 10
  • 134.
  • 135. Ultraviolet light/Fiberoptic Fluorescent Spectrometry  Fluorescence  Vital teeth fluoresce normally; necrotic & RCT teeth do not –Foreman  Lighting in the operatory fully suppressed  Patient & staff wear suitable protective goggles  Fluorescence from the pulp -substantially lower than the healthy and decayed dentin fluorescence.  Healthy and decayed dentin patterns differentiated Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
  • 136. Photoplethysmography  Optical measurement technique : blood volume changes in the microvascular bed of tissue.  Light source to illuminate the tissue & a photodetector to measure the small variations in light intensity associated with changes in perfusion Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
  • 137. Anesthetic test  L/A: painful area  Block/ infiltration/ intraosseous  Vague location of pain  Non odontogenic pain:Myocardial infarction  Differentiating between arches  PDL- identify source of pulpal pain.
  • 138.  Dentin sterilizing : Silver nitrate, phenol, eugenol & desensitizing substances  Cleansers: Alcohol, chloroform, H2O2, various acids  Restorative materials & liners Besner, Ferrigno. Practical Endodontics- A Clinical Guide
  • 139.
  • 140. Tooth surface temperature  Fanibunda: pulp circulation maintains tooth temperature  Cholesteric crystals- 10% solution in chlorinated hydrocarbon solvent(Howell et al)- non vital: lower temperature  Thermistor: vital & RCT teeth- with and without gold crowns (Banes & Hammond)  Consistent (Stoops & Scott) Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
  • 141. Tooth surface temperature  Electronic thermography: Infrared sensor, control unit, thermal image computer, software, color monitor, printer  Differences in deep & superficial areas- not sensitive  Hughes Probeye 4300 thermal video system: sensitive to measure 0.1oc  Adjunct to other diagnostic tests
  • 142. Patient temperature  Baseline temperature: follwed up  Patient is improving/ worsening  >1000oF : systemic response to infection
  • 143. Ultrasound  Compliment conventional radiography  High resolution, 3D images- inner macrostructure of the tooth  A transducer (a crystal containing probe), a coupling agent & software  Detect cracks in a simulated human tooth  Detect vertical root fractures – vital & nonvital teeth Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
  • 144. Ultrasonic Doppler Imaging  Blood circulation detected  Distinguish vital teeth from root- filled teeth: blood flow parameters, waveform, sound  Promising tool- traumatically injured teeth  Power Doppler associated with color Doppler – improved sensitivity to low flow rates Yoon et al. JOE- Volume 36, No.3, March 2010 Vital tooth Non vital tooth
  • 145. Optical Reflection Vitalometry  Preliminary report-1997 (Oikarinen et al)  Noninvasive method  The pulse of the pulp/oral mucosa.  Yet to be clinically accepted & commercially available. Tyagi et al.Saudi Endodontic Journal • May-Aug 2012 • Vol 2 • Issue 2
  • 146. Evaluation of Sensibility Tests  Thermal test: Endo Ice & EPT- evaluated  Endo Ice- 0.904 accuracy & EPT- 0.75  Age group 21-50 & vital teeth: more accurate response to cold test  Jespersen et al. JOE- Volume 40, No.3, March 2014
  • 147. RADIOGRAPHY-Little value : assess pulp status  Presence & extent of carious lesions  Vital pulp therapy  Calcifications  Resorptions  Periradicular radiolucencies  Tracing fistulous tracts  Thickness of PDL  Periodontal disease  Root & pulp space anatomy  Previous RCT
  • 148.  Bitewing: pulp chamber  Eccentric ray alignment Beer, Bauman, Kim. Color Atlas of Endodontology
  • 149. Digital radiography  Variables in diagnostic quality of conventional radiography- controlled  Image- enhanced, colorized and useful patient education tool
  • 150. Cone Beam Volumetric Tomography  First used in dentistry- Mozzo P et al 1998  Proximity to anatomic structures  Root canal anatomy
  • 151. Diagnosis: never based solely on radiographic finding
  • 153. References  Endodontic therapy – Weine  Endodntics6- Ingle et al  Cohen’s sPathways of the Pulp- 10th ed  Color Atlas of Endodontics- William T. Johnson  Endodontics- Problem solving in Clinical practice- Pitt Ford  Practical Endodontics- A clinical guide. Bessner & Ferrigno
  • 154.  Pocket Atlas of Endodontics- Beer  H. Jafarzadeh & P. V. Abbott. Review of pulp sensibility tests. Part I: general information and thermal tests. IEJ, 43, 738- 762, 2010  Yoon et al. JOE- Volume 36, No.3, March 2010  Jespersen et al. JOE- Volume 40, No.3, March 2014