Microteaching on terms used in filtration .Pharmaceutical Engineering
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Lecture 4 muscle physiology(1)
1.
2. Overview
• Structure of Muscle and Muscle Fiber
• Components of a Muscle Fiber, Myofibril,
and Sarcomere
• Structure of Myosin and Actin
• Muscle Contraction
• Muscle Fiber Types
• Fiber Type and Athletic Performance
• Muscle Fatigue during Exercise
6. • Plasmalemma- plasma membrane
– Attach to tendons
– Transport in and out of cell
– Sarcolemma includes plasmalemma and
basement membrane
MacIntosh, Gardiner, & McComas, Skeletal Muscle, Human Kinetics, 2006
Structure of a Muscle Fiber
(Muscle Cell)
7. Components of a Muscle Fiber
(Muscle Cell)
• Sarcoplasm- cytosol/cytoplasm
– Gelatin-like substance
– Storage site for glycogen, myoglobin,
and other
proteins/mineral/fats/organelles
• Transverse Tubules
– Run laterally through muscle fiber
– Path for nerve impulses (Carry action
potential deep into muscle fiber)
• Sarcoplasmic Reticulum
– Runs parallel to muscle fiber
– Calcium storage
9. Components of a Sarcomere
• Sarcomere: *Basic contractile unit of a myofibril
*Composed of interdigitating thick and thin filaments (myosin
vs. actin)
– I-Band
– A-Band
– H-Zone
– M-Line
– Z-Disk
(Z-line)
Figure 1.5
13. Components of a Sarcomere
• Sarcomere includes two types of protein
filaments
– Thick Filament: Myosin
– Thin Filament: Actin
• Alignment of the thick and thin filaments is
what give muscle its striations
14. Myosin
• Comprises 2/3 of skeletal muscle proteins
• Two protein strands twisted together
• Globular heads (Myosin Cross-bridges)
• Titin filaments stabilize myosin
15. Actin
• Thin filaments are composed of 3 proteins
– Actin: globular proteins form strands
– Tropomyosin: twists around actin strand
– Troponin: bound at intervals to actin
• Anchored to Z-Disk
19. Phases of Muscle Contraction
• Action Potential/Calcium Release
• Calcium-Troponin Binding; Tropomyosin Shift
• Actin-Myosin Binding
• Myosin Power Stroke/ ATP Binding
20. Resting Membrane Potential
(RMP)
• RMP= -70mV
• Caused by uneven separation of charged ions
inside (K+) and outside (Na+) the cell
• More ions outside the cell than inside
• Membrane more permeable to K+
• Sodium-Potassium Pumps maintain imbalance
– 3 Na+ out
– 2 K+ in
21. Ions Channels
• At rest, almost all the
Na+ channels are
closed.
• At rest, few K+
channels are open.
– Leaking due to [ ]
gradient
22. Sodium/Potassium Pump
• Resting membrane
potential is maintained by
pump
– Potassium tends to diffuse
out of cell
– Na+/K+ pump moves 3 Na+
out and 2 K+ inside the cell
– Use energy from ATP
24. Action Potential
• Occurs when a stimulus of sufficient strength
depolarizes the cell
– Opens Na+ channels, and Na+ diffuses into cell
• Inside becomes more positive
• Repolarization
– Return to resting membrane potential
• immediately following depolarization
• K+ leaves the cell rapidly
• Na+ channels close
• All-or-none law
– Once a nerve impulse is initiated, it will travel the entire
length of the neuron without losing strength. (gun shot)
Slightly
Open
Na+
Opens
Wide
Na+
27. Excitation-Contraction Coupling
(EC Coupling)
• Action potential travels to Sarcoplasmic
Reticulum, causes release of calcium into
sarcoplasm
• Calcium binds to troponin on thin filament
• Troponin moves tropomyosin, revealing
myosin binding sites on actin
• Myosin cross-bridges bind to actin
28. Muscle Excitation
1. Action potential in motor neuron
causes release of acetylcholine (ACh)
into synaptic cleft.
2. ACh binds to receptors on motor end
plate, leads to depolarization that is
conducted down transverse tubules,
which causes release of Ca+2 from
sarcoplasmic reticulum (SR).
29. Sliding Filament Theory
• Muscle contraction = muscle fiber shortening
• Myosin power stroke
– Myosin bound to actin tilts its head, pulling thin
filament towards the center of the sarcomere
– Process is repeated until Z-disk reaches myosin
filaments or until calcium is no longer available
31. Energy for Contraction
• ATP binding sites on myosin head
• ATPase (on myosin head) splits ATP into ADP
and Pi
• Energy released fuels the tilting of the myosin
head (power stroke)
• Additional ATP is required to keep contraction
going
32. Muscle Relaxation
• Calcium pumps return calcium to the SR,
stored for future use
• ATP required for calcium pumps
• Troponin and Tropomyosin return to original
position
• Thick and thin filaments return to original
positions
33. Muscle Action & Relaxation
• Muscle twitch
– Contraction as the result of a
single stimulus
– Latent period
• Lasting ~5 ms
(immediately after the stimulus)
– Contraction
• Tension is developed
• 40 ms
– Relaxation
• 50 ms
• It varies among muscle type
34. Speed of Muscle Twitch
• Speed of shortening is greater in fast fibers
– Sarcoplasmic reticulum releases Ca+2 at a faster rate
– Higher myosin ATPase activity – quicker ATP release of energy
with ATP hydrolysis
Type I
Type IIaType IIx
35. Fiber Type Characteristics
Fast Fibers Slow fibers
Characteristic Type 2x Type 2a Type 1
Number of mitochondria Low High/mod High
Resistance to fatigue Low High/mod High
Predominant energy system Anaerobic Combination Aerobic
ATPase Highest High Low
Vmax (speed of shortening) Highest Intermediate Low
Efficiency Low Moderate High
Specific tension High High Moderate
39. Fiber Type and Performance
• Power Athletes
– Sprinters
– Mostly Fast (70-75%) Twitch (Type 2)
• Endurance Athletes
– Distance Runners, Triathletes, Cyclists
– Mostly Slow (70-80%) Twitch (Type 1)
• Others
– Non-athletes
– Equal amount of Fast and Slow Twitch
40. Other Factors which Influence
Muscle Force
• Number of motor units activated
• Type of motor units activated (FT or ST)
• Muscle size
• Initial muscle length
• Joint angle
• Speed of muscle action (shortening or
lengthening)
41. Length-Tension Relationship
Figure 1.13
• Length-tension relationship
– Optimal sarcomere length = optimal overlap
– Too short or too stretched = little or no force develops
42. Speed-Force Relationship
• Speed-force relationship
– Concentric: maximal force
development decreases at
higher speeds
– Eccentric: maximal force
development increases at
higher speeds
43. Muscle Fatigue during ExerciseMuscleForceď‚®
Exercise ď‚®
<60 sec
Pi & H+
>2 hrCa Release
from SR