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Cordycepssinensis
(kida jadi)
Juhi patel
Content……
• INTRODUCTION
• MORPHOLOGY
• LIFE CYCLE
• MARKET POTENTIAL
• ARTIFICIAL CULTIVATION
• BIOACTIVE COMPOUNDS
• HEALTH BENEFITS
INTRODUCTION
• Cordyceps sinensis (CS) is one of the valuable entomophagous
fungus ( caterpillar fungus)described in traditional Chinese
medicines as rare and exotic medicinal fungi.
• In view of the many positive health effects attributed to CS, it is
hailed as wonder herb of traditional Chinese medicines since
many centuries.
• CS parasitizes caterpillar stage of ghost moths (Hepialus
armoricanus) and produces a fruiting body assessed as an herbal
remedy.
• It is mainly distributed in China, Tibetan Plateau, Bhutan, Nepal,
and the northern part of India at an altitude of 3500–5000 m
above sea level.
Host
• Scientific name: hepialus
Armoricanus
• Traditional name: ghost
moth,Himalayan bat moth
• Order: lepidoptera
• Family: Hepialidae
Worlds
most efficient
and
expensive
medicinal
fungus
Fruiting body arising
out of the dead body
of a
caterpillar
MYCOLOGICAL data
• KINGDOM: Fungi
• ORDER: Hypocreales
• FAMILY: Clavicipataceae
• GENUS: Cordyceps
• SPECIES: Cordyceps sinensis
• ENGLISH NAME: Caterpillar
fungus,Cordyceps Mushroom
• Local name: yarsa gumba,Himalayan viagra
keeda jadi, “winter worm summer grass”
• The fruiting body is dark brown to black.
• It is 5-15 cm long and 0.14 to 0.4 cm thick.
• There are two types are available in market based on the
colour. The whitish yellow is larger and good in quality. The
other type is of copper colour and it is smaller as well as
qualitatively compromised.
MORPHOLOGY
HABITAT
•Altitude:3500 to 5000 m above MSL
•Cold and arid environment
DISTRIBUTION
• Uttrakhand
• Sikkim
• Himachal pradesh
• Arunachal pradesh
• It is a fungus which grows on a larva of ghost moth.
• During the summer and early autumn , mature fruiting bodies Of
cordyceps sinensis release millions of ascospors in the air which infected
the larva and germinate inside its body.
• fungal cells spread throughout the body through the circulatory system.
• As the larva is subterranean in habit, it continues digging the soil and
enters inside from its rear part in vertical position.
• During the winter season, the fungal cells rapidly proliferate inside the
larva body and consume all the internal organs of the larva except its
exoskeleton.
• Then the fungal cells convert into the compact white mass inside the
body of larva, which is called endosclerotium.
LIFE CYCLE
• When outer temperature slowly rises up at the beginning of the
spring, the endosclerotium starts germinating and extrudes through
the head part of the larva and ultimately protrudes through the soil.
• This part called stroma gets fully mature in the summer (fruiting
bodies) and again produces ascospores, which infect the larvae in
that region. At this season the collectors start collecting this fungus.
• The life cycle needs one year to complete.
Quality parameters
• Size and firmness of the
host
• Saturated yellowish brown is preferred over pale colours
• Robustness and weight
• CS possess many health benefits such as anti-
oxidative effect, anti-tumor, potentiating immune
response, anti-inflammatory, anti-stress and anti-
fatigue,and anti-myocarditis. And also CS contains a
major class of active ingredients such as nucleosides,
polysaccharides, sterols etc.Therfore it has
tremendous scope.
• During the last decade, trade activities have
established huge market demand for CS .
MARKET POTENTIAL
LARGE SCALE PRODUCTION
Fermentation
Liquid State
Fermentation
Solid
Substrate
Surface
Fermentation
Submerged
Fermentation
ARTIFICIAL CULTURE
BASAL MEDIA
KH2PO4-1g
MgSO4 -0.5g
Thiamine0.5g
Peptone-8g
Glucose30g
pH: 5.6 to 6.0
Temperature: 18⁰C
• Mass artificial production of mycelium is done using culture
mediums, two methods of cultivation includes liquid culture
fermentation and Solid substrate method.
• Solid Fermentation :
• In this method, the substrate comprises of grains or a mixed
bag of cereals on which mycelium is allowed to grow. The grain
is usually rice, wheat or rye although many different sorts of
grains are used. Although this methodology harvests the
mycelium with maximum bio-actives recovery and this can be
well-known low price technique but disadvantage is, mycelia
contains high content of grain matter than actual CS substance.
ARTIFICIAL CULTIVATION OF CORDYCEPS SINENSIS
• Solid fermentation conditions were optimized through single
factor test to recover maximum polysaccharides where soybean
meal and rice bran (1:2 w/w) as substrates and arrived at
optimum amount of inoculation (20%), fermentation
temperature (26 C), water content of medium (60%), air
relative humidity (60%) and fermentation time (7 days).
•Liquid Fermentation :
• The nutritional requirements (17 carbohydrates, 16 nitrogen
compounds, 9 vitamins, 4 macro-elements, 4 trace-elements
and 8 ratios of carbon to nitrogen) for the successful
submerged cultivation of CS wherein a maximum of 22 g/l of
mycelial biomass could be obtained in submerged cultures after
40 days
•Carbohydrates : 29%
•Crude proteins : 29.1 to 33%
•Fiber : 18.5%
•Fat :8.4 %
•Vitamins: B1,B2,B12 & K
NUTRITIONAL FACTORS
Bioactive compounds
Cordycepin & Cordycepic acid
Amino acids- lysine, glutamic acid,
proline and threonine
Unsaturated fatty acids- 57.84% , linoleic
acid- 38.44%
Polysaccharides-3 to 8%
Nucleotides - guanosine has the
highest content
Ergosterol - mycelia (1.44 mg/g)
fruiting body (10.68 mg/g)
• Improves liver function
• Improves kidney Disease
• Reduces tumour size
• Enhances immunity
• Assists symptoms of Ageing
• Reverses HIV
• Anti-Cancer
• Anti-Oxidant
• Anti-diabetic
• Sexual and reproductive function
Major healthbenefits
Cordiceps has a significant positive effect on athletic performance
●Cordyceps has most often been used by adults in doses of 3-6
grams by mouth daily for up to 1 year.
Most cordyceps supplements are made in a lab.
Natural cordyceps is hard to get and might be expensive.
Speak with a healthcare provider to find out what dose might be
best for a specific condition.
• When taken by mouth,cordyceps might cause the immune system
to become more active. This could increase the symptoms of auto-
immune diseases. It might cause mild side effects such
as diarrhea, constipation, and stomach discomfort.If you have one
of these conditions, it's best to avoid using cordyceps.
Doses
SIDE EFFECTS
THANK YOU !!

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41. Juhi patel.pptx

  • 2. Content…… • INTRODUCTION • MORPHOLOGY • LIFE CYCLE • MARKET POTENTIAL • ARTIFICIAL CULTIVATION • BIOACTIVE COMPOUNDS • HEALTH BENEFITS
  • 3. INTRODUCTION • Cordyceps sinensis (CS) is one of the valuable entomophagous fungus ( caterpillar fungus)described in traditional Chinese medicines as rare and exotic medicinal fungi. • In view of the many positive health effects attributed to CS, it is hailed as wonder herb of traditional Chinese medicines since many centuries. • CS parasitizes caterpillar stage of ghost moths (Hepialus armoricanus) and produces a fruiting body assessed as an herbal remedy. • It is mainly distributed in China, Tibetan Plateau, Bhutan, Nepal, and the northern part of India at an altitude of 3500–5000 m above sea level.
  • 4. Host • Scientific name: hepialus Armoricanus • Traditional name: ghost moth,Himalayan bat moth • Order: lepidoptera • Family: Hepialidae
  • 5. Worlds most efficient and expensive medicinal fungus Fruiting body arising out of the dead body of a caterpillar
  • 6. MYCOLOGICAL data • KINGDOM: Fungi • ORDER: Hypocreales • FAMILY: Clavicipataceae • GENUS: Cordyceps • SPECIES: Cordyceps sinensis • ENGLISH NAME: Caterpillar fungus,Cordyceps Mushroom • Local name: yarsa gumba,Himalayan viagra keeda jadi, “winter worm summer grass”
  • 7. • The fruiting body is dark brown to black. • It is 5-15 cm long and 0.14 to 0.4 cm thick. • There are two types are available in market based on the colour. The whitish yellow is larger and good in quality. The other type is of copper colour and it is smaller as well as qualitatively compromised. MORPHOLOGY
  • 8. HABITAT •Altitude:3500 to 5000 m above MSL •Cold and arid environment DISTRIBUTION • Uttrakhand • Sikkim • Himachal pradesh • Arunachal pradesh
  • 9. • It is a fungus which grows on a larva of ghost moth. • During the summer and early autumn , mature fruiting bodies Of cordyceps sinensis release millions of ascospors in the air which infected the larva and germinate inside its body. • fungal cells spread throughout the body through the circulatory system. • As the larva is subterranean in habit, it continues digging the soil and enters inside from its rear part in vertical position. • During the winter season, the fungal cells rapidly proliferate inside the larva body and consume all the internal organs of the larva except its exoskeleton. • Then the fungal cells convert into the compact white mass inside the body of larva, which is called endosclerotium. LIFE CYCLE
  • 10. • When outer temperature slowly rises up at the beginning of the spring, the endosclerotium starts germinating and extrudes through the head part of the larva and ultimately protrudes through the soil. • This part called stroma gets fully mature in the summer (fruiting bodies) and again produces ascospores, which infect the larvae in that region. At this season the collectors start collecting this fungus. • The life cycle needs one year to complete.
  • 11.
  • 12. Quality parameters • Size and firmness of the host • Saturated yellowish brown is preferred over pale colours • Robustness and weight
  • 13. • CS possess many health benefits such as anti- oxidative effect, anti-tumor, potentiating immune response, anti-inflammatory, anti-stress and anti- fatigue,and anti-myocarditis. And also CS contains a major class of active ingredients such as nucleosides, polysaccharides, sterols etc.Therfore it has tremendous scope. • During the last decade, trade activities have established huge market demand for CS . MARKET POTENTIAL
  • 14. LARGE SCALE PRODUCTION Fermentation Liquid State Fermentation Solid Substrate Surface Fermentation Submerged Fermentation
  • 15. ARTIFICIAL CULTURE BASAL MEDIA KH2PO4-1g MgSO4 -0.5g Thiamine0.5g Peptone-8g Glucose30g pH: 5.6 to 6.0 Temperature: 18⁰C
  • 16. • Mass artificial production of mycelium is done using culture mediums, two methods of cultivation includes liquid culture fermentation and Solid substrate method. • Solid Fermentation : • In this method, the substrate comprises of grains or a mixed bag of cereals on which mycelium is allowed to grow. The grain is usually rice, wheat or rye although many different sorts of grains are used. Although this methodology harvests the mycelium with maximum bio-actives recovery and this can be well-known low price technique but disadvantage is, mycelia contains high content of grain matter than actual CS substance. ARTIFICIAL CULTIVATION OF CORDYCEPS SINENSIS
  • 17. • Solid fermentation conditions were optimized through single factor test to recover maximum polysaccharides where soybean meal and rice bran (1:2 w/w) as substrates and arrived at optimum amount of inoculation (20%), fermentation temperature (26 C), water content of medium (60%), air relative humidity (60%) and fermentation time (7 days). •Liquid Fermentation : • The nutritional requirements (17 carbohydrates, 16 nitrogen compounds, 9 vitamins, 4 macro-elements, 4 trace-elements and 8 ratios of carbon to nitrogen) for the successful submerged cultivation of CS wherein a maximum of 22 g/l of mycelial biomass could be obtained in submerged cultures after 40 days
  • 18. •Carbohydrates : 29% •Crude proteins : 29.1 to 33% •Fiber : 18.5% •Fat :8.4 % •Vitamins: B1,B2,B12 & K NUTRITIONAL FACTORS
  • 19. Bioactive compounds Cordycepin & Cordycepic acid Amino acids- lysine, glutamic acid, proline and threonine Unsaturated fatty acids- 57.84% , linoleic acid- 38.44% Polysaccharides-3 to 8% Nucleotides - guanosine has the highest content Ergosterol - mycelia (1.44 mg/g) fruiting body (10.68 mg/g)
  • 20. • Improves liver function • Improves kidney Disease • Reduces tumour size • Enhances immunity • Assists symptoms of Ageing • Reverses HIV • Anti-Cancer • Anti-Oxidant • Anti-diabetic • Sexual and reproductive function Major healthbenefits Cordiceps has a significant positive effect on athletic performance
  • 21. ●Cordyceps has most often been used by adults in doses of 3-6 grams by mouth daily for up to 1 year. Most cordyceps supplements are made in a lab. Natural cordyceps is hard to get and might be expensive. Speak with a healthcare provider to find out what dose might be best for a specific condition. • When taken by mouth,cordyceps might cause the immune system to become more active. This could increase the symptoms of auto- immune diseases. It might cause mild side effects such as diarrhea, constipation, and stomach discomfort.If you have one of these conditions, it's best to avoid using cordyceps. Doses SIDE EFFECTS
  • 22.