GRM2011: Improving groundnut productivity in Africa
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GRM2011: Improving groundnut productivity in Africa

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GRM2011: Improving groundnut productivity in Africa GRM2011: Improving groundnut productivity in Africa Presentation Transcript

  • TLI- Objective 1: Improve groundnutproductivity for marginal environmentsfrom Sub-Saharan AfricaBrief update – Important areas of progressEMBRAPA- UGA – ICRISAT – ISRA-Senegal – Malawi Nat.Prog. – Tanzania Nat. ProgHyderabad 25 Sept 2011
  • Update on groundnut objectivePerspectives for using syntheticsCurrent progress on drought
  • Sources of disease resistance:Rust : ICGV 02194, ICG 11426, ICGV 01276, ICGV 02286, and ICG 02446Rosette : ICG 14705, ICG 13099, ICG 9449, and ICG 15405ELS : ICG 6022, ICG 405, ICG 14466, ICG 6057, ICG 9449 and ICG 12509; ICG 6703; ICG10036, ICG 10384, and ICG 11219DiversitySources of drought “tolerance”:050100150200Podyield(gm-2)50 most contrasting lines0100200300400Podyield(gm-2)50 most contrasting linesWater stress IrrigatedTolerantSensitive
  • Consensus map of cultivated groundnut withdrought related QTLsGautami et al. 2011;MS under review
  • ICG721 ICGV91114ICGV02022ICGS44 ICG8751 ICGV97182ChalimbanaICG4729 ICG14482 ICG11088 ICG2772ICG8106 ICG721 ICGV91114ICGV02022ICGS44 ICG8751 ICGV97182ChalimbanaICG4729 ICG14482 ICG11088 ICG2772ICG721 0.691 t-FMPV ICGV91114 0.75 0.832 t-FMPV Fleur11 0.71 0.83 0.88 0.81 0.33 0.79 0.71 0.65 0.67 0.65 0.63 0.733 t-FMPV ICR48 0.48 0.6 0.69 0.69 0.79 0.52 0.75 0.58 0.75 0.75 0.83 0.484 t-FMPV 55437 0.75 0.79 0.96 0.81 0.65 0.73 0.85 0.63 0.65 0.67 0.67 0.795 t-germplasmICGS44 0.69 0.85 0.83 0.696 t-germplasmICG8751 0.63 0.67 0.71 0.71 0.837 t-germplasmICGV97182 0.71 0.69 0.75 0.48 0.6 0.758 t-germplasmICGV01232 0.71 0.69 0.75 0.52 0.56 0.75 0.56 0.83 0.54 0.4 0.5 0.799 t-germplasmICGV02189 0.73 0.6 0.83 0.52 0.63 0.71 0.4 0.75 0.48 0.31 0.58 0.7510 t-germplasmICG434 0.75 0.75 0.79 0.65 0.52 0.81 0.52 0.79 0.5 0.48 0.52 0.8811 s-FMPV ICGVSM87003 0.69 0.71 0.75 0.63 0.65 0.63 0.54 0.71 0.52 0.46 0.56 0.7112 s-FMPV JL24 0.58 0.79 0.54 0.75 0.83 0.79 0.75 0.88 0.71 0.63 0.67 0.7913 s-FMPV CG7 0.75 0.77 0.83 0.54 0.63 0.73 0.58 0.75 0.42 0.5 0.48 0.7914 s-germplasmICG2772 0.63 0.65 0.75 0.65 0.79 0.29 0.71 0.44 0.83 0.77 0.8315 s-germplasmICG11862 0.75 0.58 0.79 0.6 0.6 0.69 0.48 0.79 0.54 0.4 0.56 0.7916 s-germplasmICG1834 0.63 0.67 0.88 0.83 0.83 0.54 0.88 0.58 0.79 0.88 0.88 0.5417 s-germplasmICG5286 0.63 0.69 0.79 0.54 0.69 0.83 0.44 0.83 0.5 0.42 0.58 0.7718 s-germplasmICGV99001 0.69 0.52 0.73 0.56 0.79 0.52 0.58 0.65 0.63 0.58 0.75 0.5619 s-germplasmICG4598 0.73 0.81 0.58 0.75 0.79 0.65 0.83 0.67 0.88 0.85 0.75 0.6320 s-germplasmICG13787 0.6 0.75 0.88 0.69 0.5 0.73 0.63 0.71 0.67 0.56 0.69 0.77Green: TolerantYellow: Sensitive22 crosses madeDevelopment of new crosses for droughtSome of the lines used for developing SNPs
  • Rust: Three populations (JL24 x ICGV 94114, ICGV 93437 xICGV 94114 and ICGV 93437 x 95342) are being phenotyped atF5 as well as bulking up seed for second season phenotyping atF6.ELS: Two populations (ICGV-SM 95714 x Robut 33-1, ICGV-SM95714 x ICGV 93437).Rosette: Three populations (Chalimbana x ICGV-SM 90704,CG7 x ICGV-SM 90704 and JL 24 x ICGV-SM 90704) were onegeneration behind. These will be advanced through twogenerations and phenotyped at F6 in Dec – April 2012.RILs populations for disease resistanceready for genotyping / phenotyping
  • Marker-assisted introgression of rust resistance QTLPost Rainy2008-09Rainy 2010Post Rainy2010-11Rainy 2011Rainy 2009Post Rainy2009-10 BC1F1× GPBD 4F1BC2F1BC2F2BC2F3SelfingBC3F1BC3F2RPRPRPBackcrossingICGV91114(RP)×××(Crosses were made betweenGPBD 4 with ICGV 91114)BackcrossingBackcrossingSelfingSelfingGPBD4 is a cultivatedlines with earlierintrogression fromdiploid Cardenasii
  • Perspective with synthetics
  • 13579 hoehneiduranensisbenensisstenospermakempff-MercadoistenospermakuhlmanniiipaensiscardenasiimagnavalidastenospermadecorastenospermaduranensisduranensisbatizogaeastenospermaduranensisduranensisduranensisduranensisstenospermavillosaduranensishoehneistenospermakuhlmanniiduranensisduranensischiquitanaduranensisduranensisduranensisduranensisduranensisduranensiskretschmeriduranensisduranensisduranensisduranensismonticoladuranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensisduranensiscardenasiicardenasiistenospermaduranensisduranensisduranensismonticoladuranensisduranensisbatizogaeamonticolamonticolaTMV2Score of most resistant germplasm (AABB)hoehneiduranensisbenensisstenospermakempff-MercadoistenospermakuhlmanniiipaensiscardenasiimagnavalidastenospermadecorastenospermaduranensisduranensisbatizogaeastenospermaLate leaf spot scoring (1, resist – 9, suscept.) in 74 AA and BBAlmost absoluteresistance availablein diploidFrom Pande et al., 2001
  • Unlocking the genetic diversity: Amphidiploid productionAnf3 = (A. batizocoi K9484 x A. stenospermaV10309)4xAnf4 = (A. batizocoi K9484 x A. duranensisSeSn2848)4xAnf5 = (A. batizocoi K9484 x A. duranensisV14167)4xAnf6 = (A. ipaensis KG30076 x A. villosaV12812)4xAnf7 = (A. gregoryi V6389 x A stenospermaV10309)4xEMBRAPA ICRISAT
  • Runner-886 AiAdLLS damage Limited LLS damagePoor agronomic traitspod types unsuitableBC Introgression of LLS resistance from synthetics-Starting pointFrom Soraya Leal-BertioliRunner 886 Synthetic Ai x Ad
  • 10 BC1 F2 seeds from each planted in field 2008/2009Selection for LLS resistance and agronomic traits23 BC1F3 lines from 14 original BC1selected and tested for LLS in the field42 BC1 confirmed with SSR markers.12 lines from 4 original BC1being tested in field trials at twolocationsBC Introgression of LLS resistance from synthetics-Introgression scheme
  • BC1-111-10-461Runner-886BC Introgression of LLS resistance from synthetics-Proof of conceptLLS damage Resistance /Good agronomic traitspod types recoveredFrom Soraya Leal-Bertioli
  • CSSLs development: MAB from BC1 to BC4F3Ai x Ad into Fleur 11Fleur11 (A) AiAd (B) Heterozyg. (H) B or H Missing601792101702502581893216929107961061226173302402241281319377132208552425226126525451200136149509746221190109918319219839220225140244168156901251287113823421566111262152146118183187708112314101851772482492615101126102Chr 1 Chr 2 Chr 3 Chr 4 Chr 5 Chr 6 Chr 7 Chr 8 Chr 9 Chr 10 Chr 11 Chr 12 Chr 13 Chr 14 Chr 15 Chr 16 Chr 17 Chr 18Chr 19Chr 20Legend: A B Genotype 3 Genotype 4 Heterozygote Missing dataDaniel FoncekaBC1F146 -> 22 plantsBC2F1192-> 50 plantsBC3F1565-> 80 plantsBC4F1769-> 83 plantsBC4F21180-> 140 plantsBC4F31200-> 122 plantsMinimum tiling path70 plants122 lines sent to India, Niger, Malawi
  • Progress in “drought tolerance”
  • Good integrationGlasshouse – Lysimeters - FieldGetting away fromsingle trait approachComparisonto other species (Obj5)
  • 418211924222810712010824610924411024111123911223711323511423311511163117511872299111211312215123171241912521126231272512827129291303113133132351333713439135231136431374513847139491405114153142551435714459145 6114663147651486714969150711517315275153771547915581156831578515887 1598916091161931629516397164991651011661031671051682471692431702381712341722173617410175141761817722178261793018034181381824218346184501855418658187621886618970190741917819223019386194901959419698197 102198106199240200232201820216203242043220540206482075620864209722108021188212962131042142362151221628217442186021976 2209222124522220223522248443668100225226227Patancheru2Patancheru1Sadore1Sadore2PC2Genotype scoresEnvironment scoresMega-Environments418211924222810712010824610924411024111123911223711323511423311511163117511872299111211312215123171241912521126231272512827129291303113133132351333713439135231136431374513847139491405114153142551435714459145 6114663147651486714969150711517315275153771547915581156831578515887 1598916091161931629516397164991651011661031671051682471692431702381712341722173617410175141761817722178261793018034181381824218346184501855418658187621886618970190741917819223019386194901959419698197 102198106199240200232201820216203242043220540206482075620864209722108021188212962131042142362151221628217442186021976 2209222124522220223522248443668100225226227Patancheru2Patancheru1Sadore1Sadore2PC2PC1Genotype scoresMega-EnvironmentsGGE by-plotsAnalysis of drought trials across environmentsR² = 0.27040801201602000 50 100 150 200PodyieldWaterstress2009(gm-2) Pod yield Water stress 2008 (g m-2)IndiaR² = 0.00020501001502002500 50 100 150 200PodyieldWaterstress2009(gm-2)Pod Yield Water stress 2008 (g m-2)NigerNeed to take care of high G x Y under drought(MARS / GWS) Hamidou et al – Field Crops Res.
  • R2 = 0.320.000.200.400.600.801.001.201.400.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00RelativeHarvestindexLeafweightDS 1R² = 0.790.000.200.400.600.801.001.201.400.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00RelativeharvestindexLeafweightDS 2R2 = 0.53-0.200.000.200.400.600.801.001.201.400.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00RelativeHarvestindexLeafweightDS 3ToleranceindexLeaf weightLines with smallercanopy better adaptedto intermittent stressMild stress(35% less yield)Medium stress(50% less yield)Severe stress(70% less yield)Relationship between leaf area and tolerance indexRatnakumar & Vadez– Funct. Pl. Biol.
  • 01002003004005006007008009001000Leafarea(cm2)DSMost contrasting lines for leaf areaGenotypeICG 8106ICGV 02038ICG 2106ICG 3584ICG 3140ICG 4750ICGV 02022ICG 4729ICG 11088ICG 12697ICG 8567ICG 434ICGV 02189ICGV 95377ICGV SM87003ICGV 02271ICG 1834Fleur 11ICG 12879GenotypeICG 2772ICGV 97182ICGV 00350ICGV 02266ICG 10010ICG 12235ICG 15287ICG 13787ICG 9961ChalimbanaICG 4598ICG 8760ICG 2777ICG 11862ICG 1200047-10ICG 5286ICG 5663Does this work in the field?15 out of 18 top most tolerant have lower leaf area14 out of 18 top most sensitive have higher leaf areaLeaf area (LA) under drought in the fieldin 50 most contrasting lines
  • Arrows indicates Re-wateringProfile of water use from flowering to maturityWW-SensitiveWW-TolerantTolerant lines develop a smaller canopyWSRatnakumar & Vadez – Funct. Pl. Biol.
  • Thank you
  • 0.0000.0100.0200.0300.0400.0500.0600.0700.08008:0009:0010:0011:0012:0013:0014:0015:0016:0017:0018:0019:00Leafconductance(gH2Ocm-2h-1)Time of the day (H)LSD ICG 11862 ICG 12235ICG 13787 ICG 4598 ICGV 12000ICGV 02189 ICGV 02266 ICGV 11088ICGV 97182 ICGV 97183A0.000.010.020.030.040.050.060.070.0808:0009:0010:0011:0012:0013:0014:0015:0016:0017:0018:0019:00Leafconductance(gH2Ocm-2h-1)Time of the day (H)LSD Bambey-21 IT82E-18IT97K-556-6 KVX-525 UC-CB46IT84S-2049 IT93K-503-1 IT93K-693-2Mouride Suvita 2BGroundnut CowpeaFrom Issa Faye and Nouhoun Belko