Arrange the elution sequence of the following compounds by using reversed phase liquid chromatography (1st , 2nd ,3rd) Glucose, Cholesterol, Lauric aicd Solution Reversed Phase Liquid Chromatography is a chromatographic technique in which the mobile phase used is more POLAR than the NON-POLAR stationary phase. Thus hydrophilic molecules will be eluted first followed by hydrophophobic compounds. The molecules that we are considering are Glucose, Cholesterol and Lauric acid 1. Glucose: it is considered a polar molecule. The presence of oxygen atoms around the molecule in the form of -OH group will ensure the hydrophilic nature of compound. 2. Cholesterol: it is a 27-Carbon organic compound that belongs to the category of steroids. The only polar component present in the molecule is the single -OH group attached to the ring. it is counted among the group of non-polar molecule 3. Lauric acid: Lauric acid is a 12-carbon fatty acid which contains a long chin of hydrophobic group attached to the terminal polar -COOH group. It is considered to be more polar than cholesterol but it is altogether a non-polar compound due to long hydrophobic chain. As per the nature of the compounds, the sequence in which the compounds will be eluted in the reversed phase liquid chromatographic column is 1. Glucose 2. Lauric acid 3. Cholesterol .

1. Arrange the elution sequence of the following compounds by using reversed phase liquid
chromatography (1st , 2nd ,3rd)
Glucose, Cholesterol, Lauric aicd
Solution
Reversed Phase Liquid Chromatography is a chromatographic technique in which the mobile
phase used is more POLAR than the NON-POLAR stationary phase. Thus hydrophilic
molecules will be eluted first followed by hydrophophobic compounds.
The molecules that we are considering are Glucose, Cholesterol and Lauric acid
1. Glucose: it is considered a polar molecule. The presence of oxygen atoms around the molecule
in the form of -OH group will ensure the hydrophilic nature of compound.
2. Cholesterol: it is a 27-Carbon organic compound that belongs to the category of steroids. The
only polar component present in the molecule is the single -OH group attached to the ring. it is
counted among the group of non-polar molecule
3. Lauric acid: Lauric acid is a 12-carbon fatty acid which contains a long chin of hydrophobic
group attached to the terminal polar -COOH group. It is considered to be more polar than
cholesterol but it is altogether a non-polar compound due to long hydrophobic chain.
As per the nature of the compounds, the sequence in which the compounds will be eluted in the
reversed phase liquid chromatographic column is
1. Glucose 2. Lauric acid 3. Cholesterol