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Cell Division
Binary Fission, Mitosis & Meiosis
Binary Fission
•   Most cells reproduce through
    some sort of Cell Division
•   Prokaryotic cells divide through a
    simple form of division called
    Binary Fission
•   3 step process
•   Single “naked” strand splits and
    forms a duplicate of itself.
•   The two copies move to opposite
    sides of the cell
•   Cell “pinches” into two new and
    identical cells called "daughter
    cells". (Cell wall then forms if
    applicable)
Mitosis
•    Eukaryotes divide by a more complicated system called Mitosis
•    This is because:
    1. They have a nucleus which must be broken up and then
        reformed
    2. They have their DNA “packaged” in the form of Chromosomes
    3. Chromosomes are composed of Chromatin
        1. Made of DNA Strands & Proteins
    4. Also contain Nucleosomes containing Histones - Proteins the
        DNA is wrapped around Name for the DNA/Protein complex is
        Chromatin
    5. They usually have more than 1 chromosome (Humans have 23
        pairs)
    6. They have numerous organelles to equally share
Chromatin / Chromosomes
The Cell Cycle
•Most of the cell's life is spent doing
its regular function.
•Cells divide along a rough time frame
called its Cell Cycle.
•The Cell cycle consists of the
folowing steps:
•G1 (Gap 1) Phase - Cell performs its
normal function (cells which do not
divide stay in this stage for their entire
life span)
•S (Synthesis) Phase - Here the cell
actively duplicates its DNA in
preparation for division
•G2 (Gap 2) Phase - Amount of
cytoplasm (including organelles)
increases in preparation for division.
•Mitosis - Actual division occurs
Interphase
• Cell Replicates its DNA/Chromosomes
  in preparation of upcoming division




        Animal Cell           Plant cell
Prophase
1.Chromosomes Shorten
    and become visible.
                                 Animal Cell
2. Centrioles move to
    opposite sides of the cell
3. Nuclear envelope
    disappears
4. Spindle Fibers & Astral
    Fibers both together are
    known as the Spindle
    Apparatus begin to form       Plant Cell
Metaphase
• Chromosomes line up
  along center of cell       Animal Cell
  called the Metaphase
  Plate
• Chromosomes attach
  to spindle fibers
• Spindle & Astral fibers
                             Plant Cell
  are now clearly visible
Anaphase
• Centromeres break
  up separating
                            Animal Cell
  chromosome copies
• Chromosomes are
  pulled apart to
  opposite sides of cell
• Spindle & Astral fibers   Plant Cell

  begin to break down
Telophase (cytokenesis)
• Nuclear envelope forms
  around both sets of
  chromosomes                       Animal
• DNA uncoils                       Cell
• Spindle & Astral fibers
  completely disappear
   – Cytokenesis happens with
     most (but not all) cells
                                    Plant
   – Cytoplasm & organelles         Cell
     move (mostly equally) to
     either side of the cell.Cell
     Membrane “pinches” to
     form 2 separate cells
Animal Cytokeneisis
• Cytokenesis differs
  significantly between
  Animal & Plant cells.
• With animals, the
  membranes pinch
  together to form a
  Cleavage Furrow,
  which eventually
  fuses to form two
  daughter cells
Plant Cytokenesis
• With Plants, a cell wall must be formed between
  the 2 daughter cells.
• Vessicles containing Cellulose form and fuse
  between the tow daughter cells, eventually
  forming a complete cell wall.
Overview of Mitosis
Meiosis

•   Similar in many ways to mitosis
•   Several differences
•   Involves 2 cell divisions
•   Results in 4 cells with 1/2 the normal
    genetic information
Vocabulary
•   Diploid (2N) - Normal amount
    of genetic material
•   Haploid (N) - 1/2 the genetic
    material.
•   Meiosis results in the formation
    of haploid cells.
•   In Humans, these are the Ova
    (egg) and sperm.
•   Ova are produced in the
    ovaries in females
•   Process is called oogenesis
•   Sperm are produced in the
    testes of males.
•   Process is called
    spermatogenesis
Meiosis Phases
• Meiosis occurs in 2
  phases; Meiosis I, &
  Meiosis II.

• Meiosis I.
  – Prior to division,
    amount of DNA
    doubles
Crossing Over
• During metaphase 1
  homologous
  chromosomes line-up
  along the metaphase
  plate
• Areas of homologous
  chromosomes
  connect at areas
  called chiasmata
Crossing over contd.
• Crossing Over of genes
  occurs now
  – Segments of homologous
    chromosomes break and
    reform at similar locations.
  – Results in new genetic
    combinations of offspring.
  – This is the main
    advantage of sexual
    reproduction
Chromosome reduction
• During anaphase 1,
  each homologous
  chromosome is
  pulled to opposite
  sides of the cell.
  Unlike mitosis, THE
  CENTROMERES
  DO NOT BREAK.
Meiosis I continued
• Nuclei may or
  may not reform
  following
  division.
• Cytokenesis may
  or may not occur
Meiosis II
• DNA does not double
• Chromosomes randomly
  line-up along metaphase
  plate like regular mitosis.
• During anaphase 2,
  CENTROMERES
  BREAK and each
  chromosome is pulled to
  opposite sides of the cell.
• Nuclei reform and
  cytokenesis usually
  occurs (although it is
  often unequal).
Overview of Meiosis
Comparison of Mitosis & Meiosis

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Cell division

  • 1. Cell Division Binary Fission, Mitosis & Meiosis
  • 2. Binary Fission • Most cells reproduce through some sort of Cell Division • Prokaryotic cells divide through a simple form of division called Binary Fission • 3 step process • Single “naked” strand splits and forms a duplicate of itself. • The two copies move to opposite sides of the cell • Cell “pinches” into two new and identical cells called "daughter cells". (Cell wall then forms if applicable)
  • 3. Mitosis • Eukaryotes divide by a more complicated system called Mitosis • This is because: 1. They have a nucleus which must be broken up and then reformed 2. They have their DNA “packaged” in the form of Chromosomes 3. Chromosomes are composed of Chromatin 1. Made of DNA Strands & Proteins 4. Also contain Nucleosomes containing Histones - Proteins the DNA is wrapped around Name for the DNA/Protein complex is Chromatin 5. They usually have more than 1 chromosome (Humans have 23 pairs) 6. They have numerous organelles to equally share
  • 5. The Cell Cycle •Most of the cell's life is spent doing its regular function. •Cells divide along a rough time frame called its Cell Cycle. •The Cell cycle consists of the folowing steps: •G1 (Gap 1) Phase - Cell performs its normal function (cells which do not divide stay in this stage for their entire life span) •S (Synthesis) Phase - Here the cell actively duplicates its DNA in preparation for division •G2 (Gap 2) Phase - Amount of cytoplasm (including organelles) increases in preparation for division. •Mitosis - Actual division occurs
  • 6. Interphase • Cell Replicates its DNA/Chromosomes in preparation of upcoming division Animal Cell Plant cell
  • 7. Prophase 1.Chromosomes Shorten and become visible. Animal Cell 2. Centrioles move to opposite sides of the cell 3. Nuclear envelope disappears 4. Spindle Fibers & Astral Fibers both together are known as the Spindle Apparatus begin to form Plant Cell
  • 8. Metaphase • Chromosomes line up along center of cell Animal Cell called the Metaphase Plate • Chromosomes attach to spindle fibers • Spindle & Astral fibers Plant Cell are now clearly visible
  • 9. Anaphase • Centromeres break up separating Animal Cell chromosome copies • Chromosomes are pulled apart to opposite sides of cell • Spindle & Astral fibers Plant Cell begin to break down
  • 10. Telophase (cytokenesis) • Nuclear envelope forms around both sets of chromosomes Animal • DNA uncoils Cell • Spindle & Astral fibers completely disappear – Cytokenesis happens with most (but not all) cells Plant – Cytoplasm & organelles Cell move (mostly equally) to either side of the cell.Cell Membrane “pinches” to form 2 separate cells
  • 11. Animal Cytokeneisis • Cytokenesis differs significantly between Animal & Plant cells. • With animals, the membranes pinch together to form a Cleavage Furrow, which eventually fuses to form two daughter cells
  • 12. Plant Cytokenesis • With Plants, a cell wall must be formed between the 2 daughter cells. • Vessicles containing Cellulose form and fuse between the tow daughter cells, eventually forming a complete cell wall.
  • 14. Meiosis • Similar in many ways to mitosis • Several differences • Involves 2 cell divisions • Results in 4 cells with 1/2 the normal genetic information
  • 15. Vocabulary • Diploid (2N) - Normal amount of genetic material • Haploid (N) - 1/2 the genetic material. • Meiosis results in the formation of haploid cells. • In Humans, these are the Ova (egg) and sperm. • Ova are produced in the ovaries in females • Process is called oogenesis • Sperm are produced in the testes of males. • Process is called spermatogenesis
  • 16. Meiosis Phases • Meiosis occurs in 2 phases; Meiosis I, & Meiosis II. • Meiosis I. – Prior to division, amount of DNA doubles
  • 17. Crossing Over • During metaphase 1 homologous chromosomes line-up along the metaphase plate • Areas of homologous chromosomes connect at areas called chiasmata
  • 18. Crossing over contd. • Crossing Over of genes occurs now – Segments of homologous chromosomes break and reform at similar locations. – Results in new genetic combinations of offspring. – This is the main advantage of sexual reproduction
  • 19. Chromosome reduction • During anaphase 1, each homologous chromosome is pulled to opposite sides of the cell. Unlike mitosis, THE CENTROMERES DO NOT BREAK.
  • 20. Meiosis I continued • Nuclei may or may not reform following division. • Cytokenesis may or may not occur
  • 21. Meiosis II • DNA does not double • Chromosomes randomly line-up along metaphase plate like regular mitosis. • During anaphase 2, CENTROMERES BREAK and each chromosome is pulled to opposite sides of the cell. • Nuclei reform and cytokenesis usually occurs (although it is often unequal).