Crossing of two bacteriophages with mutations such as T4 rapid lysis mutant results in progeny that shows 50 colonies in E. coli B plate and 20 in E.coli K12 lambda. What is the cotransduction frequency? (1 pt) Frequency of recombinants =2 (wild type plaques in E.coli 1212 lambda) / Total number of plaques obtained in E. coli B 0.2 0.8 0.4 1.25 In sordaira, a cross between tan+ and tan- results in the following recombinants: TOTAL 1000 What is the distance of the rpo gene and the centromere 1/2 SDS/ Total progeny 1000.065mU0.87mU6.5mU A cross in the corn is carried out with three linked genes in triply heterozygous genotype for the allele pairs (A,a), (D,d) and (R,r). The dominant A results in red and aa in green leaves. The dominant D results in tall plants and dd are dwarfed. The dominant R results in ragged leaf margins and r have smooth margins. The triple heterozygous is crossed with homozygous recessive for all three alleles and the following phenotype is seen. Assume that interference in each interval is complete so the map distance in cM corresponds to the percent recombinations. red, tall, ragged 265 red, tall, smooth 24 red, dwarf, ragged 120 red, dwarf, smooth 90 green, tall, ragged 70 Green, tall, smooth 140 Green, dwarf, ragged 16 Green, dwarf, smooth 275 TOTAL 1000 1. What is the order of genes (1 point) 2. What is the distance between the genes ( 2 points) 3. Calculate interference ( 2 points) Choose an option below with all right answers R-A-D, 0.26mU for A to R,16mU for to A to D and I=66% A-R-D, 20mU for A to R,30mU for R to D,I=33% A-D-R, 20mU for D to R,30mU for A to D,1=23% When two bacterial strains use conjugation to mate. Bacterial strain met + his + leubio- was mixed with another strain met-hist-leu+bio+. But it was difficult to know the donor from the recipient. What strategy can help in distinguishing donor from recipient ( 1pt ) Use a donor strain which is antibiotic sensitive Use a donor strain which is antibiotic resistant Use a recipient strain that is antibiotic sensitive Use a U tube to separate donor and recipient with filer that can pass the DNA.