You conduct a genetic screen to identify mutant Arabidopsis plants that are more susceptible to
infection by the fungus Alternaria brassicicola compared to wild type plants. You identify an
autosomal recessive mutation (s1) that causes increased susceptibility to infection in the plants.
You test for linkage between disease susceptibility and an A T SNP located on chromosome 1 in
Arabidopsis. To genotype the SNP you design a PCR-RFLP assay. In your assay the A allele is
recognized by the restriction enzyme Hindll and the T allele is not (Results-Figure to Right).
Based on the results, what is the SNP genotype of the: Test Cross Female: Test Cross Male: ased
on the PCR-RFLP results, will you be able to use this marker for linkage analysis? (i.e. is this
marker formative in this cross). Briefly explain your answer..
You conduct a genetic screen to identify mutant Arabidopsis plants tha.pdf
1. You conduct a genetic screen to identify mutant Arabidopsis plants that are more susceptible to
infection by the fungus Alternaria brassicicola compared to wild type plants. You identify an
autosomal recessive mutation (s1) that causes increased susceptibility to infection in the plants.
You test for linkage between disease susceptibility and an A T SNP located on chromosome 1 in
Arabidopsis. To genotype the SNP you design a PCR-RFLP assay. In your assay the A allele is
recognized by the restriction enzyme Hindll and the T allele is not (Results-Figure to Right).
Based on the results, what is the SNP genotype of the: Test Cross Female: Test Cross Male: ased
on the PCR-RFLP results, will you be able to use this marker for linkage analysis? (i.e. is this
marker formative in this cross). Briefly explain your answer.
