You suspect that regulation of the transcription of the SARS-CoV-2 S Protein gene might play an important role in infection. To identify important regulatory elements for this gene, you isolate a piece of DNA that includes the sequence before the transcribed region of the gene, and you insert this potential promoter region on a piece of DNA just before the transcribed region of the GFP gene. You first take this wild-type "promoter fusion" sequence, allow transcription and translation to occur, and observe the level of GFP expression. You then make some mutations by deleting different parts of the potential promoter region, allow transcription and translation to occur, and observe the level of GFP expression. The results from your experiment are shown below. The labels above the first image below indicate the regions (A,B,C, D, and E) where your deletion mutations were made. The top promoter fusion diagraw represents the wild-type sequence. Red areas represent the potential promoter region from S Protein, the gray area is the transcribed region of the GFP gene, and gaps in the boxes are areas where your deletion mutations were made (so these regions are missing from the mutated promoter fusion). The second image shows the data you got from your experiment, with shortened labels ( M = Mutation). Using these data, mark each of the following statements true or false. GFP Expression (Relative to WT) T/F: Region C could be a promoter sequence. True False.

1. You suspect that regulation of the transcription of the SARS-CoV-2 S Protein gene might play
an important role in infection. To identify important regulatory elements for this gene, you
isolate a piece of DNA that includes the sequence before the transcribed region of the gene, and
you insert this potential promoter region on a piece of DNA just before the transcribed region of
the GFP gene. You first take this wild-type "promoter fusion" sequence, allow transcription and
translation to occur, and observe the level of GFP expression. You then make some mutations by
deleting different parts of the potential promoter region, allow transcription and translation to
occur, and observe the level of GFP expression. The results from your experiment are shown
below. The labels above the first image below indicate the regions (A,B,C, D, and E) where your
deletion mutations were made. The top promoter fusion diagraw represents the wild-type
sequence. Red areas represent the potential promoter region from S Protein, the gray area is the
transcribed region of the GFP gene, and gaps in the boxes are areas where your deletion
mutations were made (so these regions are missing from the mutated promoter fusion). The
second image shows the data you got from your experiment, with shortened labels ( M =
Mutation). Using these data, mark each of the following statements true or false. GFP Expression
(Relative to WT) T/F: Region C could be a promoter sequence. True False