Plantago major has been used traditionally to enhance wound healing. It contains several bioactive compounds like plantamajosides, acetosides, and aucubin that have wound healing properties. The leaves and seeds are applied directly to wounds or made into a juice or dressing. They have anti-inflammatory, antioxidant, and cell proliferative effects that help reduce wound size and strengthen healing. Compounds in P. major stimulate various cells involved in the stages of wound healing like hemostasis, inflammation, proliferation, and remodeling. Both ethanol and water extracts of P. major leaves have been shown to have wound healing and anti-inflammatory activities making it a potential source for alternative treatment.

1. WOUND HEALING EFFECT OF COMPOUNDS
FROM PLANTAGO MAJOR

2.  CONTENTS
 Abstract
 Introduction
 Wound
 stages of wound
 Factors affecting wound healing
 Wound healing and Plantago major
 Bioactive compounds of P. major
 Medicinal properties of P. major
 Conclusion

3. Abstract
-Why Plantago major
for wound healing?
 Medicinal properties
 Bioactive compounds
 Plantamajosides

4. Introduction
P. major derived from Latin
word ‘planta’ which means
‘sole of the foot’
Monogenic
family
Plantaginaceae
Only one genus
Plantago
approximately
256 species
Three subspecies
of P. major named
as
‘Major’
‘Intermedia’
‘winteri’.

5. Introduction
Have different habitat
requirement
Exist as everlasting
May to September is best
flower time
Anti-inflammatory activity

6. Wound
Causes
progressive
deterioration
of the tissue
disrupt the
normal structure
and function of
skin

7. Stages of Wound Healing
Hemostasis
Inflammation
Proliferation or
Granulation
Remodeling or
Maturation
Stop Bleeding
Day 1 to 3
Day 3 to 20
Week 1 to 6
Week 6 to 2 Years
New frame work for blood Vessel growth
Pulls the wound closed
Final proper tissue

8. Hemostasis
What happens?
 attempt to minimize bleeding into soft tissues
What cells are involved?
 Platelet cells
 clot formation and releasing cytokines

9. Inflammation
What happens?
• Neutrophils destroy bacteria
• Cleaning of wound
What cells are involved?
• Macrophages
• Fibroblast

10. Proliferation
What happens?
 Proliferation and migration
 Angiogenesis and granulation tissue formation
 Replacement of granular tissues
 Collagen type III
 Reduction in wound size
What cells are involved?
 Macrophages
 Fibroblasts

11. Maturation
What
happen?
Collagen type III
replace to type I
Wound color
change
What cells are
involved?
Fibroblasts My fibroblast

12. Phases of Wound Healing
Stages of
injury
Time Cells contributing in
stages
Function
Hemostasis Immediately after
injury
Platelets Blood clotting
Inflammation 1-4 days Neutrophils Phagocytosis
Macrophages
Proliferation 4-21 days Macrophages Regain skin function
Lymphocytes
Angiocytes Wound contraction
Neurocytes
Fibroblast
Remodeling 21 days to 2 years Fibrocytes Strengthening of skin

13. Factors affecting wound healing
Mechanism of
wounding
Incision, crush
Vascular
insufficiency
Loss of tissues
radiations
Underlying
disease
contamination
Aging
Immune
deficiency
mineral or Vitamin
deficiency
(zinc, vit C)
Other
human
diseases
Local factors General factors

14. Wound healing and Plantago major
• “Groblad”
• The parts of P. major that
are existing in air are used
to cure burn and other kind
of wound
• Enhances healing process
• Stop flow of blood
• Juice of P. major’s leaves.
• Bioactive wound dressing

15. Continue….
The leaves of P. major
contain number of active
compounds such as
• Plantamajoside
• Acetoside
• Aucubin
• Ursolic acid

16. Bioactive compounds of P. major responsible for
wound healing
Compounds extracted
from Plantago major
Vitamins Polysaccharides
Flavonoids
Iridoid
glycosides
Terpenoids
Alkaloids
Caffeic acid
derivatives
Fatty acids

17. Medicinal uses of P. major
Number of
diseases are
treated
P. major’s
leaves and seeds
are involved in
• wound healing
• anti-inflammation
• antioxidant
• Anticarcinogenic
• Antiviral

18. Medicinal properties of P. major
Antioxidant
Anti-
Inflammatory Anti-Stress
Antiviral
Plantago major
Antiulcerogenic
Antibacterial
Antidiabetic Anticarcinogenic

19. Conclusion
Plantago major leaves are being extracted can stimulate
cell proliferation and in vitro migration assay.
Both ethanol and water-based extracts are isolated from
fresh and dried leaves have a soothing effect that makes
Plantago major an exciting source of alternative healing
properties.
Ethanol based extraction yield more Polyphenolic
compounds as compare to water extract and have
stronger anti-inflammatory properties which has great
potential for wound healing.