The document presents information about Vibrio spp., a group of bacteria commonly found in marine and estuarine environments. Vibrio spp. are facultative anaerobes that can cause illnesses in humans and other animals. Examples of pathogenic Vibrio bacteria include those that cause cholera and vibriosis. While Vibrio infections are associated with warmer months and consumption of contaminated seafood, prevention methods focus on thoroughly cooking shellfish and washing hands and cuts after exposure to seawater.
Automated system for bacterial identificationDEEKSHANT KUMAR
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Automated system for bacterial identificationDEEKSHANT KUMAR
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2. TEXT HAS BEEN TAKEN FROM STANDARD TEXT BOOK FOR MEDICAL MICROBIOLOGY.
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Cholera is a serious bacterial disease that usually
causes severe diarrhea and dehydration. The disease is typically spread through contaminated water.
Modern sewage and water treatment have effectively eliminated cholera in most countries. It’s still a problem in countries like Asia, America and Africa. Mostly in India.
Countries affected by war, poverty, and natural disasters have the greatest risk for a cholera outbreak.
Taxonomy:
class : Gamma Proteobacteria
Order: Vibrionales
Family: Vibrionaceae
Genus: Vibrio
Species: v.cholerae, v.parahaemolyticus,
v. vulnificus, v. alginolyticus
MORPHOLOGY:
Gram negative, actively motile, short, rigid curved bacilli
Resembling letter “V”
about 34 genus
most common in water
1.5µ X 0.2 -0.4 µ in size
polar flagellum , strongly aerobic
Smear – fish in stream appearance
PATHOGENESIS:
Source: Ingestion of contaminated water, food,
fruits and vegetables etc.,
Incubation periods: 1-5 days
Symptoms: Watery diarrhoea, vomiting, thirst, dehydration, muscle cramps
Complications: muscular pain, renal failure, pulmonary edema, cardiac arrhythrnias
DIAGNOSIS:
Specimen: stool sample, water sample(envt)
Microscopy: a) Hanging drop : +ve
b) Gram stain :-ve
Culture: Mac conkey Agar :colourless to light pink
TCBS : yellow colonies
Serology: serological tests are no diagnostic value
TREATMENT:
Adequate replacement of fluids and electrolytes.
Oral tetracycline reduces the period of vibrio excreation.
PREVENTION:
Drink and use bottled water
Frequent washing
Sanitary environment
Defecate in water
Cook food thoroughly
it contains how the sample is processed and how it is subjected to staiing, biochemical reactions, what are the culture medias used, and thier methods. it also includes the antimicrobial susceptibility testing
Cholera is a serious bacterial disease that usually
causes severe diarrhea and dehydration. The disease is typically spread through contaminated water.
Modern sewage and water treatment have effectively eliminated cholera in most countries. It’s still a problem in countries like Asia, America and Africa. Mostly in India.
Countries affected by war, poverty, and natural disasters have the greatest risk for a cholera outbreak.
Taxonomy:
class : Gamma Proteobacteria
Order: Vibrionales
Family: Vibrionaceae
Genus: Vibrio
Species: v.cholerae, v.parahaemolyticus,
v. vulnificus, v. alginolyticus
MORPHOLOGY:
Gram negative, actively motile, short, rigid curved bacilli
Resembling letter “V”
about 34 genus
most common in water
1.5µ X 0.2 -0.4 µ in size
polar flagellum , strongly aerobic
Smear – fish in stream appearance
PATHOGENESIS:
Source: Ingestion of contaminated water, food,
fruits and vegetables etc.,
Incubation periods: 1-5 days
Symptoms: Watery diarrhoea, vomiting, thirst, dehydration, muscle cramps
Complications: muscular pain, renal failure, pulmonary edema, cardiac arrhythrnias
DIAGNOSIS:
Specimen: stool sample, water sample(envt)
Microscopy: a) Hanging drop : +ve
b) Gram stain :-ve
Culture: Mac conkey Agar :colourless to light pink
TCBS : yellow colonies
Serology: serological tests are no diagnostic value
TREATMENT:
Adequate replacement of fluids and electrolytes.
Oral tetracycline reduces the period of vibrio excreation.
PREVENTION:
Drink and use bottled water
Frequent washing
Sanitary environment
Defecate in water
Cook food thoroughly
it contains how the sample is processed and how it is subjected to staiing, biochemical reactions, what are the culture medias used, and thier methods. it also includes the antimicrobial susceptibility testing
Don't get sick! Get the 411 on common bugs that can impact our immune systems. How do you come in contact or prevent these types of illnesses? From food borne illnesses to the flu, we can help keep you healthy! Our guide can help.
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1. WELCOME TO OUR PRESENTATION
Group Name: VIBRIO SPP
Group members are: Johora Tabassum Munia
Abdus Sattar
Borhan Tushar
Shorap Mia
Srabony Easmin
2. Scientific Classification of Vibrio
Domain: Bacteria
Phylum: Pseudomonad Ota
Class: Gamma proteobacteria
Order: Vibrion ales
Family: Vibrionaceae
Genus: Vibrio
Pacini: 1854
Type species: Vibrio cholerae
3. What is Vibrio?
Vibrio ( genus Vibrio), any of a group of comma shaped bacteria in the family
of Vibrionaceae. Vibrios are aquatic microorganisms, some species of which
cause serious diseases in human and other animals.
Vibrios are microbiological characterized as gram-negative, highly motile,
facultative anaerobes with one to three whiplike flagella at one end. Their
sells are curved round 0.5 micrometer across and 1.5-3.0 micrometer long.
5. What is Vibrio SSP?
Vibrio spp. are a group of common, Gram-negative, rod-shaped bacteria
that are natural constituents of freshwater, estuarine and marine
environments. Vibrio spp. share several biological and genomic features.
Their genomes are divided between two chromosomes, which have been
shaped by recombination and horizontal gene transfer (HGT; that is, the
acquisition of genetic material by transfer from other organisms). Although
these pathogens may be gnomically diverse, they all originate from aquatic
and marine environments: they prefer warm, brackish (slightly salty) water,
and their abundance in the natural environment tends to mirror
environmental temperatures.
6. Where is Vibrio SSP found?
Being highly salt tolerant and unable to survive in fresh water, Vibrio spp.
are commonly found in various salt water environments. Vibrio spp. are
facultative anaerobes that test positive for oxidase and do not form
spores.
All members of the genus are motile. Cases of Vibrio spp. infections have a
marked seasonal distribution, with most cases occurring during warmer
months.
Vibrio spp. infections are usually initiated from exposure to contaminated
water or consumption of raw or undercooked contaminated seafood and
cause a variety of symptoms in humans.
7. Situation of Vibrio infection around the
world:
An estimated 3–5 million people contract cholera worldwide annually, with ~100,000 deaths; in
endemic countries, about half of the deaths occur in children of <5 years of age.
highest incidence in children of <5 years of age; about half of all cholera cases occur in this
age group, although the incidence varies annually, presumably related to climate and hitherto
unknown factors.
For centuries, cholera has been endemic in Asia, mainly in the Ganges delta of the Bay of
Bengal, Bangladesh and India. Asiatic cholera has exploded at several different times, spread
rapidly and resulted in waves of global pandemics . The spread of cholera outside of Asia.
Largely mediated by human activities. Cholera was likely introduced through infected humans
to
Africa, Haiti and Latin America, and probably also historically to Europe and the United States.
The seventh pandemic continues to be a major public health threat for 175 countries in Asia.
8. Mechanisms/pathophysiology:
Of all the studied pathogenic Vibrio spp., V. cholerae is the most well
understood. V. cholerae is
the paradigmatic non-invasive mucosal pathogen: following ingestion of
contaminated water or food by the host, the pathogen proliferates to
high density along the mucosal surface of the small intestine but does
not disrupt the integrity of the epithelial barrier or cause substantial
damage to epithelial cells.
9. Symptoms Of Vibrio SSP
Signs and symptoms of vibriosis can include watery diarrhea that is often accompanied by abdominal
cramping. Other associated symptoms and signs can include
nausea,
vomiting,
fever, and
chills.
Other vibrio infection symptoms and signs are:
Abdominal Cramping
High Fever
Watery Diarrhea
10. Prevention:
We can reduce the risk of vibriosis by following these tips:
1.Don’t eat raw or undercooked oysters or other shellfish. Cook them before
eating.
2. Always wash hands with soap and water after handing raw shellfish.
3.Avoid contaminating cooked shellfish with raw shellfish and its juices.
4.Wash wounds and cuts thoroughly with soap and water if they have been exposed
to seawater or raw seafood or its juices.
5.If develop a skin infection, meet medical provider.
References:
www.biologydiscussion.com
www.cdc.gov
www.medicinenet.com
www.nature.com
www.britannica.com
