Variants calling and SNP prioritization in mouse RNA.pptx
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This document outlines the steps for variant calling and SNP prioritization in mouse RNA sequencing datasets. It involves mapping reads to a reference genome using STAR aligner, marking duplicates and sorting reads. Variant calling is performed using GATK HaplotypeCaller. Base quality score recalibration and variant filtering are then applied. Effect predictors like VEP and SnpEff are used to annotate variants. MAFtools is used to convert variants to MAF format and analyze mutation annotation files.
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Hypertension, also known as high blood pressure, is a serious medical condition that occurs when blood pressure in the body's arteries is consistently too high. Blood pressure is the force of blood pushing against the walls of blood vessels as the heart pumps it. Hypertension can increase the risk of heart disease, brain disease, kidney disease, and premature death.
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Alcohol Use: Immediate and long-term risks include impaired judgment, health issues, and social consequences.
Tobacco Use: Immediate effects include increased heart rate, while long-term risks encompass cancer and heart disease.
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Seeking Help for Addiction: Recognizing signs, available treatments, support systems, and resources are essential for recovery.
Personal Stories: Real stories of recovery emphasize hope and resilience.
Interactive Q&A: Engage the audience and encourage discussion.
Conclusion: Recap key points and emphasize the importance of awareness, prevention, and seeking help.
Resources: Provide contact information and links for further support.
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Italy
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- 1. Variants calling and SNP prioritization in mouse RNA seq datasets MADE BY – SHIKHA ROY
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