Tissue Culture
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The Tissue Culture Shared Resource (TCSR) assists Cancer Center investigators and Georgetown University researchers with tissue culture aspects of their research. The TCSR maintains a collection of cell lines, provides reagents and testing services, assists with establishing primary cultures and large-scale tissue culture. Additionally, the TCSR offers shared tissue culture space, cryo-storage, and expert consultation and training in tissue culture techniques. The TCSR is partially supported by the Georgetown Howard University Center for Clinical and Translational Studies.
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![Tissue Culture Shared Resource (TCSR) Bassem Haddad, MD, Director [email_address]](https://image.slidesharecdn.com/tissueculture-tcsr-gu-110515085235-phpapp01/85/Tissue-Culture-1-320.jpg)
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The Georgetown Database of Cancer (GDOC) is an advanced platform that integrates clinical data with multi-omics data to help identify new therapeutic targets and biologically relevant pathways. It provides tools for gene expression analysis, pathway analysis, drug discovery, and cohort selection. GDOC has a user-friendly interface with analytic tools and quick start options to allow users to tap into its advanced capabilities for translational cancer research.
Preclinical Imaging
The Preclinical Imaging Research Laboratory (PIRL) at Georgetown University provides high-end preclinical imaging instrumentation and expertise to investigators studying cancer using animal models. PIRL offers MRI, fluorescence and bioluminescence imaging, ultrasound, and X-ray services, as well as training and assistance with imaging experiments, image analysis, and quantitative measurements. PIRL aims to support collaborative cancer research between investigators at Georgetown University and Howard University.
Electron Microscopic Image Analysis
The document summarizes the expertise and capabilities of the Laboratory for Electron Microscopic Image Analysis. The lab specializes in using electron microscopy to study the ultrastructure of the central and peripheral nervous systems. Techniques used include immunocytochemistry, histochemistry, and tracing neural circuits through anterograde, retrograde, and trans-synaptic transport of markers. Electron microscopy provides higher resolution than light microscopy to visualize subcellular structures like synapses. However, electron microscopy has disadvantages like limited sampling area and inability to label more than two elements simultaneously with color.
Microscopy and Imaging
The Microscopy & Imaging Shared Resource (MISR) provides advanced cellular imaging and image analysis services and instrumentation. MISR offers techniques like TIRF fluorescence microscopy for membrane studies, confocal microscopy for live cell imaging up to 500um deep, and spinning disk confocal for fast 3D imaging. Staff provide training, technical assistance, and access to image analysis software like Metamorph and Volocity. The goal is to support tumor biology research through quantitative imaging of subcellular changes.
Molecular Imaging
The Molecular Imaging Laboratory at Howard University provides state-of-the-art imaging equipment including high resolution MRI systems for small animal and clinical research. The lab aims to train students and foster multidisciplinary research using imaging to study disease processes and investigate new treatments. Areas of research include in vivo MRI and optical imaging of disease models in small animals, as well as molecular imaging of biological processes and developing new imaging probes and nanoparticles.
Exercise Center
The Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) provides support for the Howard University Exercise Center (HUEC) and its director Teletia Taylor. The HUEC offers services like body composition analysis, cardio-respiratory fitness testing, and exercise prescriptions. It has equipment like treadmills, bicycles and elliptical machines. Past HUEC programs examined the effects of exercise on quality of life in breast cancer survivors and prostate cancer risk factors in African American males. Support for shared resources is provided partially by GHUCCTS.
Human Physiology and Interventions
The Human Physiology and Interventions (HPI) core at Howard University, directed by Dr. Thomas Obisesan, provides exercise training, metabolic and body composition assessments, and tests of cardiovascular function. Researchers can contact Dr. Obisesan by phone or email to access these resources, which are partially supported by funding from the Georgetown Howard University Center for Clinical and Translational Studies.
Flow Cytometry
The document describes the Flow Cytometry services provided by the Translational Technology Resources at Georgetown Howard University Center for Clinical and Translational Studies. The services include access to flow cytometry equipment, analysis of user samples, cell sorting, data analysis and archiving, presentation graphics, training, consultation, and are directed by Xinbin Gu and Yanfei Zhou. Support is provided by the GHUCCTS.
Flow Cytometry/Cell Sorting
The document describes services provided by the Flow Cytometry/Cell Sorting Shared Resource (FCSR) at Georgetown University, including apoptosis assays, stem cell assays, other applications like cell cycle analysis and cytotoxicity assays, and cell sorting capabilities. The FCSR, directed by Dr. Karen Creswell, offers methods to analyze and sort stem cells using immunophenotyping and assays like Aldefluor analysis and side population detection. It also provides cell sorting into 6-, 24-, or 96-well plates and supports up to 4 populations simultaneously. The shared resource is partially supported by the Georgetown Howard University Center for Clinical and Translational Studies.
Cellular resources
The document describes various cellular resources including flow cytometry and cell sorting using a Creswell FACS Aria machine, Luminex MSD technology, microscopy imaging and cytogenetics using a Mueller microscope, TIRF and multiphoton microscopy, and tissue culture facilities managed by Haddad.
Biostatistics & Bioinformatics
The Biostatistics and Bioinformatics Shared Resource (BBSR) provides statistical analysis and bioinformatics support for basic science, clinical, and population health research projects at Georgetown University. The BBSR assists with clinical trial planning, data analysis, and monitoring. It also aids grant proposals with power and sample size calculations. The BBSR has various hardware and software resources, including servers, databases, and packages for statistical analysis, experimental design, and bioinformatics. Researchers can contact the directors or staff for assistance.
Zebrafish
The ASR Zebrafish facility provides several services including fish housing, embryo production, gene manipulation, and phenotypic analysis. Gene expression can be manipulated through RNA, DNA, and morpholino microinjections. Phenotypic analysis includes visualization of morphology, histology, fluorescence, and apoptosis. The facility also supports toxicity and drug screening in zebrafish using 96 or 384 well formats with wildtype or transgenic lines. Shared resources are partially supported by Georgetown and Howard University.
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