Prism Graph Analysis

1. Figure 1. The concentration of NAD(P)H (μM) over time (sec), during the reduction of
various flavins by AbeF.
Cofactors Rate of Reaction R2
NADH and FAD 0.2562 0.9994
NADH and FMN 0.1000 0.9972
NADH and Riboflavin 0.04009 0.9945
NADPH and FAD 0.004081 0.4490
NADPH and FMN 0.004081 0.4490
NADPH and Riboflavin 0.006319 0.7338
Table 1. The reaction rate (absolute value of slope) of AbeF in the presence of
NAD(P)H and various flavins.
The enzyme AbeF has the highest rate of reaction when in the presence of
NADH and FAD, as it oxidizes the NADH to NAD+ and reduces FAD to FADH2. The
second highest rate of reaction occurred in the presence of NADH and FMN. The third
highest rate of reaction occurred in the presence of NADH and riboflavin. This indicates
that AbeF can catalyze reactions in the presence of FMN and riboflavin but shows the
highest rate of reaction with FAD. When in the presence of NADPH, AbeF’s reaction
rate was significantly lower. There was little difference in the reaction rate when in the
presence of FAD, FMN, and riboflavin. This indicates that NADH is required for AbeF to
reduce flavins.