Bellevue School District Request for Advisory Opinion - GlucagonCharles Fournier
After 6 months' pressure from @T1DF_advocacy, @TheBSD405 has asked Washington State's nursing commission to review restriction on glucagon.
T1D Defense Fund (@T1DF_advocacy) November 18, 2015
Join us at www.t1df.org
Goal driven , compassionate professional pusuing masters in hospital management ,looking for working in hospital as trainee/employee in accreditated hospital
Bellevue School District Request for Advisory Opinion - GlucagonCharles Fournier
After 6 months' pressure from @T1DF_advocacy, @TheBSD405 has asked Washington State's nursing commission to review restriction on glucagon.
T1D Defense Fund (@T1DF_advocacy) November 18, 2015
Join us at www.t1df.org
Goal driven , compassionate professional pusuing masters in hospital management ,looking for working in hospital as trainee/employee in accreditated hospital
Detection of tetB Gene in Environmental Escherichia coli Strains Using Colony...AnissaBoyers
Tetracycline is a commonly used broad spectrum antibiotic that targets the bacterial ribosome and inhibits protein synthesis. Bacterial resistance to tetracycline can occur via drug efflux from the cell, changes in the ribosomal binding site, or enzymatic modification of the drug. These mechanisms are driven by the expression of tet genes. tet genes can be acquired in pathogenic and commensal Escherichia coli, which is a Gram negative, coliform, rod-shaped bacterium that is found in the intestinal tracts of animals. A particular tet gene of interest is tetB, which is in plasmid DNA or the chromosome and codes for an efflux pump. Our goal was to detect the tetB gene in 14 environmental E. coli strains using the colony polymerase chain reaction (PCR) and to determine the minimum inhibitory concentration (MIC) of tetracycline that would inhibit growth in each strain.
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1. Anissa Boyers
226 Red Fox Ct, McGaheysville, VA 22840
aboyers@eagles.bridgewater.edu
+1 (540) 421 5115
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Objective
An intrinsically motivated, forthcoming graduate of Bridgewater College with a B.S. in Biology eagerly seeks entry
into the School of Clinical Laboratory Science at Augusta Health Center – Fishersville, VA. Detail oriented and
passionate about providing the service of healthcare to a diverse range of individuals. Experience performing an
array of laboratory techniques in a BSL-2 lab setting.
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Education
Bridgewater College, Bridgewater, VA August 2018 – Present
B.S. Biology, Pre-Health Sciences Track, Minor: Neuroscience
Lantz Ace Endowed Scholarship, Charles & Linnie Buckle Endowed Scholarship
Relevant coursework in General Biology and General Chemistry, Genetics, Organic Chemistry, Molecular Biology,
Human Anatomy, Human Diversity, Human Physiology, Biochemistry, Immunology, Microbiology, Calculus,
Statistics, Psychopharmacology,Neuroethics
Laboratory Techniques
Quality control, aseptic technique. PCR, ELISA, Western blot, bacterial isolation, pipetting, H&E staining, Gram
staining, acid-fast staining, endospore staining, capsule staining, general use of instrumentation, spectrophotometry
Software: Excel, GraphPad Prism, PyMOL, BLAST, Adobe Photoshop and Lightroom
Research
Tetracycline Resistance in Escherichia coli strains collected from North River and November 2021
Stone Village Pond
Collaborated with a group of students in collecting water samples and isolating 14 E. coli strains. Zones of
inhibition observed from an agar disk diffusion assay.Large Plasmid extraction was performed, and gel
electrophoresis showed the possible presence of plasmid DNA in 7 out of 14 strains, which is possibly
correlated with the tet(B) gene.
PCR analysis of Tetracycline Resistance Genes in Environmental Escherichia coli strains Present
PCR to detect tet(B) and tet(M) genes in 14 environmental strains of E. coli. Quantitative observation of
level of resistance at various concentrations of tetracycline (μg).
Professional Experience/Development
Phone interviewed Casey Hartless, a biology alumnus of Bridgewater College and March 2021
a Clinical Laboratory Scientist at Augusta Health Center – Fishersville, VA
Professional Organizations
American Society for Microbiology (ASM) January 2022
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Work Experience
Lifeguard, Great Eastern Resort – Massanutten,VA May 2017 – August 2020
Certified in adult, child, and infant CPR, AED, First Aid, and Oxygen Administration
Collaborated with co-workers to perform rescues for endangered patrons and provided appropriate medical care
Observed patrons closely and enforced rules to prevent emergencies
Mentored new employees in job duties, provided swimming and diving instruction to children, cleaned swimming
pool and facilities
Rewarded for exceptional performance after being audited by the American Red Cross
Campus Recreation, Bridgewater College – Bridgewater, VA August 2021 – Present
Certified in CPR, Bloodborne pathogens training
Greets and checks in students,faculty,staff members, and guests
Maintains cleanliness of weight room, performs safety inspections on equipment, engages with the community
through campus recreation events,enforces rules to ensure safety of patrons
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Collegiate Involvement,Bridgewater College, Bridgewater, VA
Inter-district Youth Cabinet January 2020 – Present
A student run organization at Bridgewater College that is affiliated with the Church of the Brethren. Collaborates
with other cabinet members to plan events for 100+ brethren youth.
2. Anissa Boyers
226 Red Fox Ct, McGaheysville, VA 22840
aboyers@eagles.bridgewater.edu
+1 (540) 421 5115
_____________________________________________________________________________________________
Recording Secretary: May 2020 – May 2021
Attentively took detailed notes that encapsulated the points of interest during weekly meetings
Inter-district Youth Cabinet Coordinator: May 2021 – Present
Develops agenda and leads weekly meetings, recruits potential new members
Active Minds Chapter August 2018 – Present
A national organization that spreads awareness and provides resources for suicide prevention and mental health on
Bridgewater College’s campus.
Vice President: August 2021 – Present