This study investigated the effects of Talinum paniculatum (ginseng jawa) on the internal organs (liver, kidney, and intestine) of 5 mice (Mus musculus) over 20 days. The mice were given 0.5g of Talinum paniculatum leaves mixed in 100ml of water daily. The mice's weight and water consumption were measured daily. After 20 days, the organs were dissected and tissue slides were prepared for microscopic analysis. The slides showed effects in the intestine, liver, and kidney tissues, such as increased mucus secretion in the intestine, enlarged liver sinusoid spaces, and tightly packed kidney glomeruli. The study suggests Talinum paniculatum consumption affected the

1. TALINUM PANICULATUM (GINSENG JAWA) 20 DAYS

2. Group members… AMIRAH BINTI SHAARI HILDA ANAK EMAK AIN NAJWA BINTI AMRAN NURUL HIDAYAH BINTI MANSOR ONG SHWU CHYN PUI WAN TING WONG SIEW CHING NATASHA KAUR AMARPREET KAUR YAASHINI SELVARAJAH KRISNAVIMALA KRISHUAN NABILAH BINTI YUSOF NORIZA BINTI ISHAK

3. INTRODUCTION

4. FiveMusmusculushave been used to investigate the effect of TalinumPaniculatum(ginseng jawa) to the liver, kidney and intestine within ` 20 days of experimental.

6. Easy to get

8. Significance of study TalinumPaniculatumcontains vitamin c and toxic Tanin, Saponin and Flavoid. So we carry out this experiment to see the side effects of TanilumPaniculatum to the internal organs (intestine, kidney and liver) of Musmusculus.

9. Research limitation Experiment applicable only to five Musmusculus. only three leaves of TanilumPaniculatum used everyday. Time limitation to only 20 days

10. LITERATURE REVIEW

11. 1.Talinum:A Summer Vegetable For Florida DAVID FAIRCHILD, COCONUT GROOVE, FLORIDA

12. DR J.L HEID said that in his report : In the green leaves ofTalinum The Vitamin C is about 160 mg per 100 gram The Talinumshow the phenomenally high content of vitamin C compared to guavas and sweet peppers. It also probably has a considerable contents of carotenoid pigments.

13. 2The Effect Of Ginseng Jawa Root (TalinumPaniculatum) To The White Male MencitEviSetiyaManuputyFaculty Of Pharmacy University Of Muhammadiyah Surakarta

14. Chemical content in Talinumpaniculatumcomposed of saponin, flavanoid and tanin. Saponin - strengthen our body and - improve blood circulation. Saponin : can cause irritation in mucosa membrane, and can damage red blood cell through hemolysis

15. The root can be used to treat : tiredness, dizzy, diarrhea, aphrodisiacs and pneumonia. The leaves can be used to treat bump. Also increase the sexual desire in male mencit.

16. Materials and methods

17. 20 days of observation 5 mice (Musmusculus) Bedding 3 ginseng jawa (Talinumpaniculatum) leaves (0.5g) mix with 100 ml water Mice food Mice is weighed daily Amount of water consumed are measured

18. Dissection Glove Cotton and chloroform Dissecting set Dissecting tray and pins Water Beaker Water was applied on the ventral side of mice The kidney, small intestine and liver were dissected.

19. Stages/technique in preparing slide Fixation. Dehydration. Clearing. Embedding. Sectioning. Coloring/ staining.

20. Fixation Bouin’s fluid (<24 hours) Bottles Dehydration Ethanol 70% (< 24 hours) Ethanol 80%, 95%, 100% (< 1 hour) Clearing Toluene solution (< 24 hours)

23. Soaking of slides-

24. into 21 piece of jar containing different types of chemicals and liquid

25. based on the duration given.

26. Gluing: DPX / Canada balsam

27. Heating of slides

28. (37 C)

29. 1 weekXylen 1 And 2 Alcohol+xylen (1:1) Alcohol, Alcohol( 90%,80%,75%) Distilled Water Hematoksilin Solution 0.5 % Sodium Bicarbonate Solution Running Water Scott’s Solution Eosin

30. Results & discussion

31. Graph showing the increasing weight mass of the mice in 20 days

32. The weight of each mice increase day by day After taking Talinumpaniculatum as their drink. The Talinumpaniculatum rich with vitamin C increases appetite Gaining of body weight

33. Graph showing the amount of water and Talinumpaniculatumconsumed by the mice's

34. Intestine (duodenum) Affected intestine 40x magnification Goblet cell The goblet cell in this intestine secretes more mucous compared to the normal intestine

35. LIVER Affected liver Normal slide 40 x magnification sinusoid Wide spaces in between cells showing the sinusoid. This means that more enzymes are secreted to detoxify the drug (ginseng jawa). In the normal slide the sinusoid is compact.

37. Materials & function:

38. Weight gain they will get attracted to mate becomes active and aggressive

40. The end.. Thank you..