 NATASHA KAUR
 AMARPREET KAUR
 YAASHINI
SELVARAJAH
 KRISNAVIMALA
KRISHUAN
 NABILAH BINTI
YUSOF
 NORIZA BINTI
ISHAK
...
Five Mus musculus have been
used to investigate the
effect of
(ginseng jawa)
to the liver, kidney and
intestine within `
o...
Why do we choose
Mus musculus as our specimen?
• Class of mammalian
• Easy to get
• Easy to handle-small in size
To know the effect of Talinum
Paniculatum
size and
body weight of Mus musculus
Behaviour investigation of the
Mus musc...
 Talinum Paniculatum contains
 vitamin c and
 toxic
 Tanin,
 Saponin and
 Flavoid.
 So we carry out this experiment...
Experiment applicable only to five
Mus musculus.
only three leaves of Tanilum
Paniculatum used everyday.
Time limitatio...
DAVID FAIRCHILD,
COCONUT GROOVE,
FLORIDA
DR J.L HEID said that in his report :
In the green leaves of Talinum
 The Vitamin C
 is about 160 mg per 100 gram
The...
 Chemical content in Talinum paniculatum
composed of
 saponin,
 flavanoid and
 tanin.
 Saponin - strengthen our body ...
 The root can be used to treat :
 tiredness,
 dizzy,
 diarrhea,
 aphrodisiacs and
 pneumonia.
 The leaves can be us...
 5 mice (Mus musculus)
 Bedding
 3 ginseng jawa (Talinum paniculatum) leaves (0.5g)
mix with 100 ml water
 Mice food
...
 Glove
 Cotton and chloroform
 Dissecting set
 Dissecting tray and pins
 Water
 Beaker
 Water was applied on the ve...
Fixation.
Dehydration.
Clearing.
Embedding.
Sectioning.
 Coloring/ staining.
Fixation
 Bouin’s fluid (<24 hours)
 Bottles
Dehydration
 Ethanol 70% (< 24 hours)
 Ethanol 80%, 95%, 100% (< 1 hour...
Embedding
 (Paraffin processing)
 Paraffin solution (60C) (< 1 hour)
 Paraffin solution (60C) (< 1 hour)
• (twice proc...
materials methods
 Diamond pen
 Distilled water
 Albumin
 Slide and slide cover
 Microtome
 Brush
 The slices was p...
Materials Methods
 Xylen 1 And 2
 Alcohol+xylen (1:1)
 Alcohol,Alcohol( 90%,80%,75%)
 DistilledWater
 Hematoksilin So...
0
5
10
15
20
25
30
1
2
3
4
5
Graph showing the increasing weight mass of the mice in 20 days
The weight of each mice
 increase day by day
After taking Talinum paniculatum as
their drink.
The Talinum paniculatum
...
Graph showing the amount of water and Talinum paniculatum consumed by the mice's
The goblet cell in this intestine secretes more mucous compared to the normal
intestine
40x magnification
Goblet cell
Wide spaces in between cells showing the sinusoid. This means that more enzymes
are secreted to detoxify the drug (ginseng...
 The presence of vitamin C in Talinum
paniculatum causes more enzyme to be
released to detoxify the drugs in the liver
ce...
The glomerular is tightly packed due to the toxic caused by the drug that is being
detoxify by the glomerular as compared ...
• act as rinsing agent to remove the blood
from the organs, and its sterileNormal saline solution
• a fixing & preserving ...
Effect of
Talinum
paniculatum
Increase the
appetite
Improve
blood
circulation
Stimulate
testosterone
hormone to
enhance
se...
 Tannins
 precipitate proteins
 inhibits in some ruminant animals
for the absorption of nutrients from high-tannin
grai...
Damage of the weighing machine.
Damage of Microscope.
Thank you..
Talinum paniculatum
Talinum paniculatum
Talinum paniculatum
Talinum paniculatum
Talinum paniculatum
Talinum paniculatum
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Talinum paniculatum

  1. 1.  NATASHA KAUR  AMARPREET KAUR  YAASHINI SELVARAJAH  KRISNAVIMALA KRISHUAN  NABILAH BINTI YUSOF  NORIZA BINTI ISHAK  AMIRAH BINTI SHAARI  HILDA ANAK EMAK  AIN NAJWA BINTI AMRAN  NURUL HIDAYAH BINTI MANSOR  ONG SHWU CHYN  PUI WAN TING  WONG SIEW CHING
  2. 2. Five Mus musculus have been used to investigate the effect of (ginseng jawa) to the liver, kidney and intestine within ` of experimental.
  3. 3. Why do we choose Mus musculus as our specimen? • Class of mammalian • Easy to get • Easy to handle-small in size
  4. 4. To know the effect of Talinum Paniculatum size and body weight of Mus musculus Behaviour investigation of the Mus musculus.
  5. 5.  Talinum Paniculatum contains  vitamin c and  toxic  Tanin,  Saponin and  Flavoid.  So we carry out this experiment to see the side effects of Tanilum Paniculatum to the internal organs (intestine, kidney and liver) of Mus musculus.
  6. 6. Experiment applicable only to five Mus musculus. only three leaves of Tanilum Paniculatum used everyday. Time limitation to only 20 days
  7. 7. DAVID FAIRCHILD, COCONUT GROOVE, FLORIDA
  8. 8. DR J.L HEID said that in his report : In the green leaves of Talinum  The Vitamin C  is about 160 mg per 100 gram The Talinum show the phenomenally high content of vitamin C compared to guavas and sweet peppers. It also probably has a considerable contents of carotenoid pigments.
  9. 9.  Chemical content in Talinum paniculatum composed of  saponin,  flavanoid and  tanin.  Saponin - strengthen our body and - improve blood circulation.  Saponin : can cause irritation in mucosa membrane, and can damage red blood cell through hemolysis
  10. 10.  The root can be used to treat :  tiredness,  dizzy,  diarrhea,  aphrodisiacs and  pneumonia.  The leaves can be used to treat bump.  Also increase the sexual desire in male mencit.
  11. 11.  5 mice (Mus musculus)  Bedding  3 ginseng jawa (Talinum paniculatum) leaves (0.5g) mix with 100 ml water  Mice food  Mice is weighed daily  Amount of water consumed are measured
  12. 12.  Glove  Cotton and chloroform  Dissecting set  Dissecting tray and pins  Water  Beaker  Water was applied on the ventral side of mice  The kidney, small intestine and liver were dissected.
  13. 13. Fixation. Dehydration. Clearing. Embedding. Sectioning.  Coloring/ staining.
  14. 14. Fixation  Bouin’s fluid (<24 hours)  Bottles Dehydration  Ethanol 70% (< 24 hours)  Ethanol 80%, 95%, 100% (< 1 hour) Clearing  Toluene solution (< 24 hours)
  15. 15. Embedding  (Paraffin processing)  Paraffin solution (60C) (< 1 hour)  Paraffin solution (60C) (< 1 hour) • (twice procedure)  Paraffin solution added in mold followed by the organ.  Tissue sample then was left for 5 days.  The infiltrating medium (paraffin) provides cellular support for cutting tissue sections.
  16. 16. materials methods  Diamond pen  Distilled water  Albumin  Slide and slide cover  Microtome  Brush  The slices was placed on the slide.  Few drops of distilled water/albumin are dropped on the slides.  Intestine-distilled water  Albumin - liver and kidney  Heating (30C)
  17. 17. Materials Methods  Xylen 1 And 2  Alcohol+xylen (1:1)  Alcohol,Alcohol( 90%,80%,75%)  DistilledWater  Hematoksilin Solution  0.5 % Sodium Bicarbonate Solution  RunningWater  Scott’s Solution  Eosin  Color stain used : • Harris Hematoksilin- Eosin coloring. • Soaking of slides- • into 21 piece of jar containing different types of chemicals and liquid • based on the duration given. • Gluing: DPX / Canada balsam • Heating of slides • (37 C) • 1 week
  18. 18. 0 5 10 15 20 25 30 1 2 3 4 5 Graph showing the increasing weight mass of the mice in 20 days
  19. 19. The weight of each mice  increase day by day After taking Talinum paniculatum as their drink. The Talinum paniculatum  rich with vitamin C increases appetite Gaining of body weight
  20. 20. Graph showing the amount of water and Talinum paniculatum consumed by the mice's
  21. 21. The goblet cell in this intestine secretes more mucous compared to the normal intestine 40x magnification Goblet cell
  22. 22. Wide spaces in between cells showing the sinusoid. This means that more enzymes are secreted to detoxify the drug (ginseng jawa). In the normal slide the sinusoid is compact. sinusoid 40 x magnification
  23. 23.  The presence of vitamin C in Talinum paniculatum causes more enzyme to be released to detoxify the drugs in the liver cells.  prove: white spaces (sinusoid)seen in effected liver cells while the original cell the (sinusoid) is more compact.
  24. 24. The glomerular is tightly packed due to the toxic caused by the drug that is being detoxify by the glomerular as compared to the normal kidney slide. glomerulus 40x magnification
  25. 25. • act as rinsing agent to remove the blood from the organs, and its sterileNormal saline solution • a fixing & preserving solution for the organsBouin’s solution- • For dehydration processEthanol 70%,80%,95% & 100% • used for the clearance purpose Toluene • to hold the organ tissues tightly during the sectioning process paraffin • Only the small intestine cell can withstand with the pressure.Distilled water • to maintain the shape of the kidney and liver cell.Albumin
  26. 26. Effect of Talinum paniculatum Increase the appetite Improve blood circulation Stimulate testosterone hormone to enhance sexual desire they will get attracted to mate becomes active and aggressive Weight gain
  27. 27.  Tannins  precipitate proteins  inhibits in some ruminant animals for the absorption of nutrients from high-tannin grains such as sorghum.  Tannic acid does not affect absorption of other trace minerals such as zinc, copper, and manganese in rats  Prove: the glomerulus at kidney cell is tightly packed compared to the original slide of kidney where there are some spaces between the glomerulus.  The lethal dose of Talinum paniculatum around 6% is able to increase the animal's body weight.  Prove: the mice’s body weight increases drastically in that 20 days.
  28. 28. Damage of the weighing machine. Damage of Microscope.
  29. 29. Thank you..

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