Five Mus musculus have been
used to investigate the
to the liver, kidney and
intestine within `
Why do we choose
Mus musculus as our specimen?
• Class of mammalian
• Easy to get
• Easy to handle-small in size
To know the effect of Talinum
body weight of Mus musculus
Behaviour investigation of the
Talinum Paniculatum contains
vitamin c and
So we carry out this experiment to see the
side effects of Tanilum Paniculatum to the
internal organs (intestine, kidney and liver) of
Experiment applicable only to five
only three leaves of Tanilum
Paniculatum used everyday.
Time limitation to only 20 days
DR J.L HEID said that in his report :
In the green leaves of Talinum
The Vitamin C
is about 160 mg per 100 gram
The Talinum show the phenomenally high
content of vitamin C compared to guavas
and sweet peppers.
It also probably has a considerable
contents of carotenoid pigments.
Chemical content in Talinum paniculatum
Saponin - strengthen our body and
- improve blood circulation.
Saponin : can cause irritation in mucosa
membrane, and can damage red blood cell
The root can be used to treat :
The leaves can be used to treat bump.
Also increase the sexual desire in male mencit.
5 mice (Mus musculus)
3 ginseng jawa (Talinum paniculatum) leaves (0.5g)
mix with 100 ml water
Mice is weighed daily
Amount of water consumed are measured
Cotton and chloroform
Dissecting tray and pins
Water was applied on the ventral side of mice
The kidney, small intestine and liver were
Paraffin solution (60C) (< 1 hour)
Paraffin solution (60C) (< 1 hour)
• (twice procedure)
Paraffin solution added in mold followed by the organ.
Tissue sample then was left for 5 days.
The infiltrating medium (paraffin) provides cellular
support for cutting tissue sections.
Slide and slide cover
The slices was placed on
Few drops of distilled
water/albumin are dropped
on the slides.
Albumin - liver and
Xylen 1 And 2
0.5 % Sodium Bicarbonate
Color stain used :
• Harris Hematoksilin- Eosin
• Soaking of slides-
• into 21 piece of jar
containing different types of
chemicals and liquid
• based on the duration given.
• Gluing: DPX / Canada balsam
• Heating of slides
• (37 C)
• 1 week
Graph showing the increasing weight mass of the mice in 20 days
The weight of each mice
increase day by day
After taking Talinum paniculatum as
The Talinum paniculatum
rich with vitamin C
Gaining of body weight
Graph showing the amount of water and Talinum paniculatum consumed by the mice's
The goblet cell in this intestine secretes more mucous compared to the normal
Wide spaces in between cells showing the sinusoid. This means that more enzymes
are secreted to detoxify the drug (ginseng jawa). In the normal slide the sinusoid is
40 x magnification
The presence of vitamin C in Talinum
paniculatum causes more enzyme to be
released to detoxify the drugs in the liver
prove: white spaces (sinusoid)seen in effected liver
cells while the original cell the (sinusoid) is more
The glomerular is tightly packed due to the toxic caused by the drug that is being
detoxify by the glomerular as compared to the normal kidney slide.
• act as rinsing agent to remove the blood
from the organs, and its sterileNormal saline solution
• a fixing & preserving solution for the organsBouin’s solution-
• For dehydration processEthanol 70%,80%,95% &
• used for the clearance purpose
• to hold the organ tissues tightly during the
• Only the small intestine cell can withstand
with the pressure.Distilled water
• to maintain the shape of the kidney and liver
they will get attracted to mate becomes active and aggressive
inhibits in some ruminant animals
for the absorption of nutrients from high-tannin
such as sorghum.
Tannic acid does not affect absorption
of other trace minerals such as zinc, copper, and
manganese in rats
Prove: the glomerulus at kidney cell is tightly packed compared to
the original slide of kidney where there are some spaces between
The lethal dose of Talinum paniculatum around
6% is able to increase the animal's body weight.
Prove: the mice’s body weight increases drastically in
that 20 days.
Damage of the weighing machine.
Damage of Microscope.