Synthesis of penicillin can be achieved by its retro-synthetic analysis. Penicillin is an antibiotic which is effective against wide range of ailment. It consist of beta lactam ring and acyl group in its structure. There are different types of Penicillin based on the different nature of the alkyl (R) group attached at the beta lactam ring. Retrosynthetic analysis of any molecules made it synthesis more easier. By doing retrosynthetic analysis, strategic point of breaking bond can be identified