While several studies have focused on native advertisements’ disclosures, little research has been conducted on native advertisement’s content. Therefore, this experimental study investigated the influence of both disclosures and brand presence (as a content factor) on readers’ persuasion knowledge (PK) and subsequent evaluations. Online news readers (N = 290) were shown a native advertisement with either a top-, middle, or top-and-middle positioned disclosure and either high or low brand presence. Results showed that middle-positioned disclosures were most often recognized, resulting in higher conceptual PK. Yet, disclosure recognition did not affect attitudinal PK and evaluations. Conversely, high brand presence did not increase readers’ conceptual PK, but it did increase attitudinal PK, which subsequently decreased readers’ evaluations of the ad, brand and news medium. These findings have important implications to both news media and advertisers.