Status of disease in the STATE NUMBER 4 on the basis of EPIDEMIOLOGICAL STUDY and laboratory cases

1. Prepared by:
Dr. Krishna Prasad Acharya
Veterinary Officer
Regional Veterinary Laboratory, Pokhara
Presented by:

2. 18 10 4 2
29 18 28
6 17
425
5
154
6
31
286
41
0
50
100
150
200
250
300
350
400
450
No. of Outbreak
No. of Outbreak

3. Disease outbreak situation in western region-
2017
0
50
100
150
200
250
300
350
400
450
FY 2015
FY 2016

4. -
Poultry : 2060 negative for Coccidia
390 positives for Coccidia (15.91%)
– 1 sample was found positives for the Demodex canis


5. Total Sample Tested: 300
Positives: 238 Negative : 62
: Kaski-Lwanghelal VDC (Ward 9), Shiding
(Positive 90%), Ghana Pokhara-Lamjung (Positive 80%), Mustang
District Jomsom,Thini (Positive 68%)
Strongyloides, Strongylus and Monezia sps

6. Clinical Mastitis in Cattle and Buffaloes
at RVL, Pokhara
In Clinical Mastitis cases clients, technicians brings
samples to the RVL for the cultural examination.
– SLST test Positive milk samples = 340 (66.40%)
– SLST test Negative Milk Samples = 172 (33.59%)
– SLST Positive milk of Buffaloes = 121 (35.29%)
– SLST Positive milk of Cattle = 219 (64.41%)
Total Sample Received : 512 Animals

7. 71
103
70
72
24
No growth
E.Coli
Streptococcus Spp
Staphylococcus spp
Bacillus sps
N= 340
Microbiological Examinations………..
Bacteria isolated in the Clinical Mastitis milk samples

8. Antibiogram of Mastitis Milk Samples
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
R
MS
HS

9. Post Mortem and Lab Diagnosed Poultry Diseases
Diseases Total Percentage
Colibacillosis 823 25.37
CRD 280 8.63
Coccidiosis 390 12.02
Infectious Bursal Disease 440 13.56
Salmonellosis 140 4.31
Mycotoxicosis 450 13.87
Avian Nephritis 75 2.31
New Castle Disease 108 3.33
Omphalitis 250 7.70
Avian Enteritis 90 2.77
Ascariasis 33 1.01
Others 164 5.05
Total cases (n) = 3243

10. Avian Influenza Rapid Test
Total Tested 2914
• Flu A Positive - 23
• Negative - 2891
• H9 -23 confirmed by CVL
Sudden Heavy Mortality
Positive AIV test Kit

11. Temporal distribution of Flu A positive cases in RVL from
069/70 to 073-74
5
7
4
3
10
8
6
0
1
5
0
2
4
1
0 0 0 0 0
1
2
4
3
00 0 0 0 0
1
0 0
3
7
4
2
0
2
1
0
1
2 2
0
3
11
3
2
0
2
4
6
8
10
12
69/70
70/71
71/72
72/73
73/74

12. Virological Examinations
Species
Number
of Swab
Tested For
Test
Method
Applied
Results
Goats 112 PPR Penside test 74 +ve
Dog/Cattle/Buffalo/
Goat/Cat/Pig/
Monkey/Squiral
36 Rabies Rapid test 7+ve
Poultry 165 IBD
IBDV rapid
test
120+ve
Poultry 78 Newcastle d/s
NDV rapid
test
12 +ve

13. Serological Examinations
Species
Number of
Serum
Tested For
Test
Method
Applied
Results
Cattle 160 Brucellosis RBPT All Negative
Buffalo 7 Brucellosis RBPT All Negative
Goats 23 Brucellosis RBPT All Negative
Poultry 365 Salmonellosis PAT 170+ve
Poultry 269 Mycoplasmosis PAT 25+ve

14. 14
Kapilvastu : Hematological and biochemical
parameters (n=45)
Parameters Values Indications Possible causes or
diseases
RBC 5.76 In range
WBC 5.8 In range
Neutrophil 28.36 In range
Lymphocytes 63.19 In range
Eosinophil 4.14 In range
Monocyte 4.24 In range
Basophil ------
Platelets ------
PCV 25.12 In range
HB 8.25 In range
Blood sugar 11.43 Low Hypoglycemia
Total serum protein 6.10 In range
Phosphorus 7.30 In range
Calcium 4.31 Low Hypocalcemia

15. Gorkha : Hematological and
biochemical parameters (n=97)
Parameters Obtained
References ( Koshihara
2006; Jain 1993) Result
RBC million/mm3 5.975± 0.171 5.0-10.0 In Range
WBC thousand/mm3 5.953 ± 0.393 4-12 In Range
Hemoglobin % 9.130 ±0.159 8.0-15.0 In Range
PCV % 27.644± 0.483 24.0-46.0 In Range
Neutrophil % 33.80±1.573 15.0-47.0 In range
Lymphocyte % 55.289±1.486 45.0-75.0 In range
Eosinophil % 4.889±0.323 2.0-20.0 In range
Monocyte % 5.790±0.539 2.0-7.0 In range
Basophil % 0 0-2.0
In
Range

16. Gorkha : Serological parameters
Obtained Result
References ( Jain 1993;
Koshihara 2006) Indications
Parameters
Blood sugar 11.08 ± 1.063 Very low 35.0-55.0 Hypoglycemia
Protein mg/dl 7.515 ± 0.154 In Range 5.7-8.1
Phosphorus
mg/dl 6.384 ±0.219 In Range 4.0-8.0
Calcium mg/dl 5.702 ± 0.165 Low 8.0-12.5 Hypocalcemia

17. Parvat : Hematological and
parameters (n=39)
Obtained
References (Koshihara 2006;
Jain 1993) Result
RBC million/mm3 5.136± 0.26 5.0-10.0 In Range
WBC thousand/mm34.92 ± 0.32 4-12 In Range
Haemoglobin % 9.05 ±0.97 8.0-15.0 In Range
PCV % 27.55± 3.17 24.0-46.0 In Range
Neutrophil % 36.70±1.18 15.0-47.0 In range
Lymphocyte % 52.33±1.53 45.0-75.0 In range
Eosinophil % 5.02±0.323 2.0-20.0 In range
Monocyte % 5.39±0.42 2.0-7.0 Range
Basophil % 0 0-2.0 In Range

18. Parvat: Serological parameters
Obtained Result
References ( Jain
1993; Koshihara
2006) Indications
Parameters
Blood sugar 6.42 ± 1.50 Very low 35-55 Hypoglycemia
Protein mg/dl 7.40 ± 0.61 In Range 5.7-8.1
Phosphorus
mg/dl 5.53 ±0.48 In Range 4.0-8.0
Calcium mg/dl 6.60 ± 0.81 Low 8.0-12.5 Hypocalcemia

19. Parasites
Gorkha: No helminthes seen, only B. coli seen
 Parvat: out of 24 fecal samples tested, 12 were
negative, 1 positive for Paramphistomum, and 11
positive for B. coli.

20. Recommendation
Immediate measures to restore normal blood
calcium and glucose level is recommended.

21. Facilities needed for further improvement of
Animal Disease Diagnosis
There is a need of disease diagnostic centre (BSL-1) in
Besisahar, Lamjung to cover two districts- Lamjung and
Manang; in Beni Myagdi for Myagdi and Mustang; in
Baglung for Parvat and Baglung; in Pokhara for Syangja and
Kaski; in Gorkha for Tanahu and Gorkha and in Kawasoti for
Nawalpur District.

22. • Provision of at least one Basic Safety Level-1 (BSL-
1) laboratory in each rural municipality.
• One Bio-safety level-2 ( BSL-2) laboratory for
disease diagnosis in this province.

23. Thank you !!!!!