Second quarter progress report regional veterinary laboratory Pokhara

1. Prepared by:
Dr. Krishna Prasad Acharya
Veterinary Officer, Regional Veterinary Laboratory, Pokhara, Nepal
Presented By:
Dr. Kedar Raj Pande
Second Quarter Progress Report
2073/74
Regional Veterinary Laboratory, Pokhara

2.  Second Quarter (Mangsir-Falgun) Progress
Report 2073/74
 Epidemiological status of the Western
Development Region- Six-monthly
 Major laboratory services of RVL
 Progress on Good Governance

3. Program
Budget
head
ANNUAL
Budget
(000)
Second quarter
progress
Weightage
Progress(%)
Financial
Progress(%)
Regular
(Working) 3271013 10076 89.74% 88.21%
Regular
(Capital) 3271014 22600
sfd e}/x]sf] e'Qmflg
lbg af+ls
Progress Summary

4. ah]6 sfo{qmd
jflif{s
jh]6-x_
l4ltorf}dfl;s k|ult
j]?h'
lgsf;f vr{
#@&!)!#
rfn'
kz':jf:Yo
;]jf
!))&^ 3175953.76
3175953.76
%}g#@&!)!$
k'+hLut
kz':jf:Yo
;]jf
@@^))
sfd e}/x]sf] e'Qmflg lbg af+ls
lgsf;f tyf vr{

5. ah]6 sfo{qmd
aflif{s
ah]6 -x_
ci6dfl;s k|ult
a]?h'
lgsf;f vr{
#@&!)!#rfn'
kz'
:jf:Yo
;]jf
!))&^ 5431974.21
5431974.21
5}g
#!@!@%$
k'+hLut
kz'
:jf:Yo
;]jf
@@^)) 1076741.53
1076741.53
bf];|f]rf}dfl;s
sfd e}/x]sf]eQmfgL lbgaf+sL

6. sfo{qmd ah]6 lzif{s aflif{s ah]6
-x_
ci6dfl;s k|ult
efl/tk|ult ljlQo k|ult
kz' :jf:Yo ;]jf #!@!@%#
rfn'
!))&^ 85=90% 78=49%
kz' :jf:Yo ;]jf
#!@!@%$
k'+hLut
@@^)) 92% 39=70%
sfde}/x]sf]
e'QmfgL lbg af+ls

7.  Second Quarter Progress Report 2072/73
 Epidemiological Status of western development
region-Six Monthly
 Major laboratory services RVL
 Good Governance

8. District Number of reports
received in VEC/RVL
Tanahun, Kaski, Syangja,
Palpa, Gulmi, Parbat,
Baglung, Manang,
Lamjung, Gorkha,
Myagdi, Rupandehi,
Nawalparasi,
Arghakhanchi
6
Kapilbastu, Mustang 5

9. S.N District Sample
Species
Date Test
methods
Remarks
1 Animal
Quarentine
Office
Rupendehi
Goat Mansir Pen-side test 25 samples
17 Sample+ve,
PPR
2 Lamjung Mongoose Poush,Magh Rabies rapid-
kit test
All 2 samples
+ve
3 Lamjung Dog Chaitra Rabies rapid-
kit test
1 Sample -ve
4 Tanahun Goat Poush Pen-side test 10 sample,
7+ ve
5 Kaski Goat Poush,Magh Pen-side test 16 samples,
13+ve
6 Syangja Goat Phalgun Pen-side test 6 All Sample
+ve

10. 31
3 3 2
85
0
10
20
30
40
50
60
70
80
90
FMD PPR Sheep and
Goat Pox
CSF ND

11. 2 2 1
59
14
91
11
82
1
8
0
10
20
30
40
50
60
70
80
90
100

12. 2
138
7
0
20
40
60
80
100
120
140
160
BQ Coccidiosis Enterotoxaemia

13. District Major diseases
Manang Coccidiosis
Mustang ND
Gorkha None
Lamjung PPR, FMD,ND
Kaski FMD, PPR, ND, Rabies,IBD
Syangja PPR, ND, FMD
Parbat FMD, ND, PPR
Baglung Coccidiosis, IBD
Myagdi Fowl Pox, Coccidiosis
Tanahun IBD, Coccidiosis

14. District Major diseases
Arghakhanchi PPR, Fowl Pox,
Gulmi IBD, Coccidiosis
Rupandehi Coccidiosis,
Kapilavastu ND, FMD,IBD,Fowl Pox,
Nawalparasi FMD, ND, IBD
Palpa FMD, ND,IBD

15.  PPR Vaccine
No. of vaccines Stock 2073/1/22 : 96,550
No. distributed till Date 073/3/32: 12300
No. distributed till 1st quarter of 073/074: 11800
No. distributed till 2nd quarter of 073/074: 7500
Remaining Stock: 64950
 Anti Rabies vaccine
No. of vaccines received till date: 3295
No. distributed till Date 073/3/32: 900
No. distributed till 1st quarter of 072/073: 320
No. distributed till 2nd quarter of 072/073: 97
Expired vaccines: 1978

16.  Second Quarter progress Report 2072/73
 Epidemiological Status of western
development region-Second quarter
 Major laboratory services RVL
 Good Governance

17. l;=g
+
sfo{qmdsf] gfd kl/df0f k|ult %
! /utdf k|f]6f]hf]jf kl/If0f !$$ !))
@ lSlnlgsnx]df6f]nf]lhsn k/LIf0f !$$ !))
# jfof]s]ldsn k/LIf0f !@) !))
$ lk;fjkl/If0f %! !))
% zjkl/If0f !@(( !))
^ lhjf0f' sNr/ tyf cfO;f]n];g !%@ !))

18. -
Positives: 82 for Coccidia (13.35%)
 Skin scrapings examination : 4 samples from dog
◦ 1 sample was found positives for the Demodex
◦ 3 samples found Negative
 Total Sample Tested = 614 intestinal scrapings of poultry
Parasitological Examinations

19. Total EPG 100
Positive: 80 Negative : 20
• Site of sample collection: Ghane Pokhara,Lamjung
.Major parasite egg reported:
 Strongyloides (78%/100)
 Trichostrongyle (44%/100)
 Monezia (12%/100)
 Trichuris (08%/100)

20. • Samples for testing Clinical Mastitis brought by
clients, and technicians for clinical
◦ SLST test Positive milk samples = 90 (66.17%)
◦ SLST test Negative Milk Samples = (33.82 %)
Total Sample Received : 136 animals

21. Microbiological Examinations...
* 37 Plastic bottled samples were not
cultured
27
7
5
1
0
5
10
15
20
25
30
E.coli Streptococcus Staphylococcus Bacillus

22. Antibiogram of Mastitis milk
samples
37
15
37
33
4
33
26
4 5
0
8
0 0
3
0
5
10
15
20
25
30
35
40
Noofsamplestested
Antibiogram of milk tested
HS
MS
R12
53
41
0
10
20
30
40
50
60
No growth Culture Antibiotics
sensitivity
Noofsamples

23. Diseases Total Percentage
Colibacillosis 239 26.38
CRD 63 6.93
Coccidiosis 74 8.14
Infectious Bursal Disease 100 11.01
Salmonellosis 30 3.30
Mycotoxicosis 225 24.77
Immunsupression 14 1.54
New Castle Disease 14 1.54
Omphalitis 38 4.18
Ascariasis 10 1.10
Avian Influenza 7 0.77
Others 64 7.04
Total cases (n) = 908
Post mortem and lab diagnosed poultry diseases

24. Species
Number of
Sample
Tested For
Test Method
Applied
Results
Goats 58 PPR
Pen side
test
44 +ve
Dog/Buffalo/
Mongoose
7 Rabies Rapid test
5 +ve
Buffalo-
2/Mongoose
-3
Poultry 26 IBD
IBDV rapid
test
11+ve
Poultry 12 Newcastle d/s
NDV rapid
test
3 +ve
Poultry 1332 Avian Influenza
AIV rapid
test
7 +ve
5 H9 /2 H5
Virological Examinations

25. Species
Number of
Serum
Tested For
Test Method
Applied
Results
Cattle 68 Brucellosis RBPT All Negative
Buffalo 20 Brucellosis RBPT All Negative
Dog 3 Brucellosis RBPT All Negative
Poultry 134 Salmonellosis PAT 25 +ve
Poultry 71 Mycoplasmosis PAT 14 +ve
Serological Examinations

26.  Conducted ELISA for Swine fever on
Pig serum samples collected on FY
2073/74
 District: Kaski
 Nepal Livestock Bangur Farm
Purunchour.
 Sample tasted: 41
 Positive Samples :38
 Negative Samples :3

27.  District :Tanahun
 Manaslu Pig Farm,Suklagandaki-1.
 Multiple Agro Farm,Suklagandaki-10 .
 Udhamshil yuba
A.sahakari,Suklagandaki-10
 Samples tested: 51
 Positive samples:43
 Negative Samples :8

28.  Second Quarter Progress Report 2072/73
 Epidemiological status of western
development region-Second quarter
 Major laboratory services RVL
 Good Governance

29. ◦ Ifltk'lt{ ;lxtsf] gful/s j8fkqsf] Joj:yf ePsf]
◦ ph'/L÷u'gf;f] k]6Lsfsf] Joj:yf ePsf]
◦ ;'rgfkf6Ldf dfl;s vr{sf] kmf+6jf/L 6f+; ug{] u/]sf]
◦ sfof{nodf ;'zf;g OsfO{ u7g eO{ sfdsfh u/]sf]
◦ ;fj{hlgs vl/b OsfO{ ;ldlt u7g ePsf]
◦ ;'rgf clwsf/L / u'gf;f] ;'Gg] clwsf/L tf]lsPsf] .
;'zf;gsf]ljj/0f

30. kb >]l0f ;d'x l:js[t
b/jGbL
kbk'lt{ nf]=;]=cf=d
f dfu e}
uPsf]
l/Qm kb ;+Vof
a=k=lr /f=k=lå= -k|f=_ slif÷e]6]/Lg/
L
! !
k=lr= /f=k=t[= -k|f=_ slif÷e]6]/Lg/
L
@ ! !
k=:jf=k|f= /f=k=cg+=k| -k|f slif÷e]6]/Lg/
L
@ @
gf=k=:jf=k
|f
/f=k=cg+=lå= -
k|f=_
slif÷e]6]/Lg/
L
# #
n]vfkfn /f=k=cg+=k|= n]vf÷k|zf;g ! !
v/Lbf/ /f=k=cg+=lå= k|zf;g ! !
6fOlki6 /f=k=cg+=k|= k|zf;g ! !
hgzlQm ljj/0f

31.  3 ongoing programs
 Herd health of animals being monitored
 Hematological and biochemical
parameters being examined
 Study on reduced fertility of farm
animals
 Clinical Mastitis tested on the collected
milk samples

32.  Target: Kapilvastu district on 1st
quarter and Gorkha on 2nd quarter
 Current Progress:
 45 blood samples, 45 serum samples and
43 milk samples tested for Gorkha.

33.  Current Progress:
 45 blood samples, 45 serum
samples and 23milk samples
tested of Gorkha tested and
analyzed
 Haematological tests and
Biochemical tests of 50 samples
from Gorkha ongoing

34. Obtained
References (
Koshihara 2006;
Jain 1993) Result
RBC million/mm3 5.975± 0.171 5.0-10.0 In Range
WBC thousand/mm3 5.953 ± 0.393 4-12 In Range
Haemoglobin % 9.130 ±0.159 8.0-15.0 In Range
PCV % 27.644± 0.483 24.0-46.0 In Range
Neutrophil % 33.80±1.573 15.0-47.0 In range
Lymphocyte % 55.289±1.486 45.0-75.0 In range
Eosinophil % 4.889±0.323 Low
Monocyte % 5.790±0.539 2.0-7.0 Range
Basophil % 0 0-2.0
In
Range

35. Obtained Result
References (
Jain 1993;
Koshihara 2006)Indications
Parameters
Blood sugar 11.08 ± 1.063 Very low 35-55 Hypoglycemia
Protein mg/dl 7.515 ± 0.154 In Range 5.7-8.1
Phosphorus
mg/dl 6.384 ±0.219 In Range 4.0-8.0
Calcium mg/dl 5.702 ± 0.165 Low 8.0-12.5 Hypocalcemia

36. wGojfb!!!