Staining for Conventional Light Microscopy
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Define stain and staining. Explain what is achieved with staining. Explain how staining for conventional light microscopy take place. Explain some basic terms on stainingExplain the causes and solutions of the problems commonly faced in staining. Cite examples of specific tissue components stained with special stain. Dr Farhana Taher Sumya Guided by: Prof Khondkar Manzare Shamim
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Staining for Conventional Light Microscopy
- 2. Stain StainingStain What is stain and staining?
- 3. Unstained tissue Stained tissue Why do we stain? Why do we stain?
- 4. Dr Farhana Taher Sumya Guided by: Prof Khondkar Manzare Shamim Dept. of Anatomy, BSMMU STAINING For Conventional Light Microscopy
- 5. What should the audience be a able to do after attending this session? 1. Define stain and staining 2. Explain what is achieved with staining 3. Explain how staining for conventional light microscopy take place 4. Explain some basic terms on staining
- 6. What should the audience be a able to do? 5. Explain the causes and solutions of the problems commonly faced in staining 6. Cite examples of specific tissue components stained with special stain
- 8. Supravital staining: the cells with the dark blue dots and curved linear structures (reticulum) are reticulocytes. How can we stain?
- 9. Nonvital staining How can we stain?
- 10. T T ST T T S M S SA How can we stain?
- 11. T SS How can we stain? Physical staining Chemical staining
- 12. Monochrome stain Dichrome stain How many colours do we need
- 13. Metachromasia How many colours do we need
- 14. T Acid (-) S Base (+) T Base (+) S Acid (-) T Acidophilic Basophilic Do we understand basophilic and acidophilic?
- 15. Do we understand basophilic and acidophilic?
- 16. T Acid (-) S Base (+) P(NH) Base (+) S Acid (-) DNA Acidophilic Basophilic 2 Do we understand basophilic and acidophilic?
- 17. Acid (-) P(-cooh) Base (+) S Do we understand basophilic and acidophilic?
- 18. Do we understand basophilic and acidophilic?
- 19. Acidophilic or basophilic Do we understand basophilic and acidophilic?
- 20. Acidophilic or basophilic Do we understand basophilic and acidophilic?
- 21. Acidophilic and basophilic Do we understand basophilic and acidophilic?
- 22. Acidophilic or basophilic Do we understand basophilic and acidophilic?
- 23. Dark or pale Does dark mean blue?
- 24. Dark or pale Does dark mean blue?
- 25. Dark or pale Does dark mean blue?
- 26. Dark or pale Does dark mean blue?
- 27. Dark or pale Does dark mean blue?
- 28. Dark or pale Does dark mean blue?
- 29. Dark or pale Does dark mean blue?
- 30. Acidophilic and basophilic Can we identify tissue types with naked eye?
- 31. Can we identify tissue types with naked eye?
- 32. Can we identify tissue types with naked eye?
- 33. Inadequate staining What problems do we face in staining? Clearing by xylene
- 34. Uneven staining What problems do we face in staining? Proper time maintaining and dehydration
- 35. Overstaining with hematoxylin What problems do we face in staining? Acid Alcohol
- 36. Understaining with eosin What problems do we face in staining? Adding acetic acid
- 37. Overstaining with eosin What problems do we face in staining? Washing with aldehyde tap water Or 95% alcohol
- 38. Opacity What problems do we face in staining? Complete dehydration
- 39. Presence of black component in section What problems do we face in staining? Filtering stain
- 40. Is routine staining enough?
- 41. Collagen Fiber H and E stain Mallory stainMasson trichome Is routine staining enough?
- 42. Silver StainH and E stain Reticular fiber Is routine staining enough?
- 43. H and E stain Elastic fibre Van gieson stain Weigert’s Resorcin fuchsin Is routine staining enough?
- 44. Goblet cell H and E Alcian bluePAS stain Is routine staining enough?
- 45. Glomerular basement membrane PAS stain H and E stain Is routine staining enough?
- 46. Glycocalyx membrane of villus H and E PAS stain Is routine staining enough?
- 47. H and E Mallory Azan Islets of Langerhans Is routine staining enough?
- 48. Congo red Amyloid H and E Is routine staining enough?
- 49. Feulgen stains DNA H and E Is routine staining enough?
- 50. Fat stain (osmium tetraoxide) Fat stain (Oil red O stain) Fat H and E Is routine staining enough?
- 51. So, what did we discuss today?
- 52. Thank You
- 54. Golgi apparatus stains dark from heavy metal ions in nerve tissue
Editor's Notes
- Inadequate dewaxing
- Due to xylene remaining in tissue uneven stain
- Understained with eosin
- Incomplete dehydration,so section is opaque
- If any airbubble during mounting or presence of blue metallic component becz of using hematoxylin wthout filtring