This document discusses a study on the anti-tumor effects of ferulic acid on human lung cancer cells. The study found that ferulic acid inhibited the growth and migration of H1299 lung cancer cells in a concentration-dependent manner. Specifically, ferulic acid treatment reduced cell viability, induced cell cycle arrest, decreased reactive oxygen species levels, and lowered the activity of matrix metalloproteinases -9 and -2 which are involved in cancer cell migration and invasion. These results suggest that ferulic acid has potential as a natural chemopreventative compound against lung cancer.
El documento describe cómo la inflación y el alto costo de vida en Argentina están aumentando constantemente, lo que lleva a sueldos escasos y mayor pobreza. La Organización Mundial del Comercio cuestiona la política económica del gobierno argentino. A pesar de la riqueza del territorio argentino, la pobreza es visible en las calles debido a la mala gestión económica del gobierno.
El documento describe los tipos de almacenamiento en la nube, incluyendo público, privado e híbrido. También discute los principales servicios de almacenamiento en la nube como Dropbox, Google Drive y SkyDrive, comparando sus características como capacidad de almacenamiento gratuita, máxima capacidad contratable, tamaño máximo de archivo y capacidades de sincronización y colaboración. El documento explica que estos servicios permiten a los usuarios acceder y compartir archivos de forma remota desde cualquier dispositivo con conexión a Internet
Dokumen tersebut memberikan informasi tentang pentingnya karakter dan personal mastery bagi seorang salesperson untuk memberikan layanan terbaik kepada konsumen. Terdapat juga tips tentang cara memperlakukan konsumen dengan hormat dan membantu mereka tumbuh secara finansial atau emosional.
El documento proporciona información sobre las instalaciones y actividades ofrecidas en el Planetario de Puebla, México. Incluye una descripción del planetario, sus horarios y costos, así como detalles sobre talleres, proyecciones, juegos interactivos y exhibiciones permanentes sobre temas astronómicos y científicos que buscan entretener e informar a los visitantes.
El documento habla sobre la adopción de mascotas. Recomienda adoptar en lugar de comprar para evitar la sobrepoblación de animales en refugios. Explica que al adoptar se le da un hogar a un animal que de otra forma podría terminar en la calle o ser explotado. También menciona algunas instituciones como Pedigree y Cambia un Destino que ayudan a encontrar hogares para las mascotas que buscan ser adoptadas.
Standard Competence of CEFR for LanguagesIne Purwanti
This document outlines the CEFR (Common European Framework of Reference for Languages) levels of competence for spoken interaction and production. It describes the abilities and range of language for each level from A1 (basic) to C2 (mastery). For spoken interaction, higher levels indicate greater fluency, ability to participate actively in discussions, and skill in relating contributions to other speakers. For spoken production, as the levels increase, descriptions become more detailed and complex, with clearer structure and logical organization of ideas. Vocabulary and discourse markers also expand in range and sophistication at higher levels.
El documento resume un proyecto de investigación realizado por tres estudiantes sobre la acidificación de los océanos. El equipo investigó cómo la acidificación de los océanos afecta a la especie humana. Realizaron una búsqueda de información utilizando términos clave y fuentes confiables, analizaron los hallazgos, y concluyeron que la acidificación de los océanos tiene consecuencias negativas para los seres humanos y el medio ambiente.
El documento describe cómo la inflación y el alto costo de vida en Argentina están aumentando constantemente, lo que lleva a sueldos escasos y mayor pobreza. La Organización Mundial del Comercio cuestiona la política económica del gobierno argentino. A pesar de la riqueza del territorio argentino, la pobreza es visible en las calles debido a la mala gestión económica del gobierno.
El documento describe los tipos de almacenamiento en la nube, incluyendo público, privado e híbrido. También discute los principales servicios de almacenamiento en la nube como Dropbox, Google Drive y SkyDrive, comparando sus características como capacidad de almacenamiento gratuita, máxima capacidad contratable, tamaño máximo de archivo y capacidades de sincronización y colaboración. El documento explica que estos servicios permiten a los usuarios acceder y compartir archivos de forma remota desde cualquier dispositivo con conexión a Internet
Dokumen tersebut memberikan informasi tentang pentingnya karakter dan personal mastery bagi seorang salesperson untuk memberikan layanan terbaik kepada konsumen. Terdapat juga tips tentang cara memperlakukan konsumen dengan hormat dan membantu mereka tumbuh secara finansial atau emosional.
El documento proporciona información sobre las instalaciones y actividades ofrecidas en el Planetario de Puebla, México. Incluye una descripción del planetario, sus horarios y costos, así como detalles sobre talleres, proyecciones, juegos interactivos y exhibiciones permanentes sobre temas astronómicos y científicos que buscan entretener e informar a los visitantes.
El documento habla sobre la adopción de mascotas. Recomienda adoptar en lugar de comprar para evitar la sobrepoblación de animales en refugios. Explica que al adoptar se le da un hogar a un animal que de otra forma podría terminar en la calle o ser explotado. También menciona algunas instituciones como Pedigree y Cambia un Destino que ayudan a encontrar hogares para las mascotas que buscan ser adoptadas.
Standard Competence of CEFR for LanguagesIne Purwanti
This document outlines the CEFR (Common European Framework of Reference for Languages) levels of competence for spoken interaction and production. It describes the abilities and range of language for each level from A1 (basic) to C2 (mastery). For spoken interaction, higher levels indicate greater fluency, ability to participate actively in discussions, and skill in relating contributions to other speakers. For spoken production, as the levels increase, descriptions become more detailed and complex, with clearer structure and logical organization of ideas. Vocabulary and discourse markers also expand in range and sophistication at higher levels.
El documento resume un proyecto de investigación realizado por tres estudiantes sobre la acidificación de los océanos. El equipo investigó cómo la acidificación de los océanos afecta a la especie humana. Realizaron una búsqueda de información utilizando términos clave y fuentes confiables, analizaron los hallazgos, y concluyeron que la acidificación de los océanos tiene consecuencias negativas para los seres humanos y el medio ambiente.
El documento describe los bombardeos atómicos de las ciudades japonesas de Hiroshima y Nagasaki por parte de Estados Unidos en agosto de 1945. Mientras que el presidente Truman argumentó que la bomba atómica puso fin a la guerra y salvó vidas, un sobreviviente japonés ofrece un testimonio del horror y destrucción que presenció en Hiroshima después del ataque.
La escuela en la Sociedad de la Información. La escuela 2.0Andrea Caro
El documento describe las transformaciones de las instituciones educativas en la Sociedad de la Información. La escuela debe transformarse para enseñar el aprendizaje permanente y la creación en lugar de la reproducción, adoptando características de la "escuela 2.0" como el aprendizaje colaborativo y el uso de tecnología. Esto requiere cambios como nuevas competencias para los estudiantes, roles para los profesores, y estructuras organizativas más flexibles como las redes de formación.
Este documento descreve a origem e o desenvolvimento da filosofia grega desde seus primórdios no século VI a.C. com Tales de Mileto até o período sistemático no século IV a.C. com Aristóteles. A filosofia grega é dividida em quatro períodos principais: pré-socrático, socrático, sistemático e helenístico. Os filósofos gregos estabeleceram as bases do pensamento racional, científico e ético que influenciaram decisivamente o
The document discusses various financial ratios used to analyze the profitability and efficiency of Atlas Battery Company over the past 5 years. It analyzes ratios such as gross profit ratio, operating ratio, net profit margin, return on equity, current ratio, inventory turnover, and cash conversion cycle. Most of the ratios for Atlas Battery have remained steady over the past 5 years, however some like operating ratio and net profit margin are not as high as they could be, indicating room for improved expense management and increased profits. The overall financial position of Atlas Battery appears stable based on favorable ratios such as return on equity, current ratio, and cash conversion cycle.
This document provides a summary of Mai Abd El-Monsef Sayed's professional experience and qualifications. She has over 10 years of experience in human resources management and is currently seeking a challenging career in the field. Her experience includes roles as an HR Manager, Senior Associate, and Generalist for various engineering, consulting, and food industry companies in Egypt and Kuwait. She has strong skills in recruitment, compensation, training, and performance management.
El documento discute el concepto de manifestaciones culturales. Explica que las manifestaciones culturales son actividades públicas y colectivas que producen un acto de comunicación para identificar a un grupo. También explica que una obra individual solo adquiere significado cultural cuando se hace pública y se comunica colectivamente. Además, describe brevemente algunas manifestaciones de la cultura peruana como Caral, Sechín, Chavín e Incas.
The resume summarizes Jay Prabhakaran's objective of working in a challenging environment to help achieve organizational goals. It provides details of his address, contact information, and dates of employment. Jay has over 10 years of experience in customer service and quality assurance roles for insurance companies. He seeks to leverage his analytical skills and certifications in Lean Six Sigma and quality auditing. His education includes an Insurance Foundation Certificate and Professional Certificate in Insurance from institutions in Ireland.
This document describes constructing deletion mutants of several genes in the uropathogenic E. coli strain UTI89. The key steps are:
1. PCR was used to generate gene disruption cassettes for spr, prc, eco293-sinI, and fimS.
2. The cassettes were electroporated into UTI89 cells expressing Red recombinase and plated on chloramphenicol to select mutants.
3. PCR confirmed successful disruption of the spr gene, replacing it with the cassette.
4. The cassette was then evicted by expressing FLP recombinase from pCP20, leaving only the gene deletion.
5. The process
El documento describe los bombardeos atómicos de las ciudades japonesas de Hiroshima y Nagasaki por parte de Estados Unidos en agosto de 1945. Mientras que el presidente Truman argumentó que la bomba atómica puso fin a la guerra y salvó vidas, un sobreviviente japonés ofrece un testimonio del horror y destrucción que presenció en Hiroshima después del ataque.
La escuela en la Sociedad de la Información. La escuela 2.0Andrea Caro
El documento describe las transformaciones de las instituciones educativas en la Sociedad de la Información. La escuela debe transformarse para enseñar el aprendizaje permanente y la creación en lugar de la reproducción, adoptando características de la "escuela 2.0" como el aprendizaje colaborativo y el uso de tecnología. Esto requiere cambios como nuevas competencias para los estudiantes, roles para los profesores, y estructuras organizativas más flexibles como las redes de formación.
Este documento descreve a origem e o desenvolvimento da filosofia grega desde seus primórdios no século VI a.C. com Tales de Mileto até o período sistemático no século IV a.C. com Aristóteles. A filosofia grega é dividida em quatro períodos principais: pré-socrático, socrático, sistemático e helenístico. Os filósofos gregos estabeleceram as bases do pensamento racional, científico e ético que influenciaram decisivamente o
The document discusses various financial ratios used to analyze the profitability and efficiency of Atlas Battery Company over the past 5 years. It analyzes ratios such as gross profit ratio, operating ratio, net profit margin, return on equity, current ratio, inventory turnover, and cash conversion cycle. Most of the ratios for Atlas Battery have remained steady over the past 5 years, however some like operating ratio and net profit margin are not as high as they could be, indicating room for improved expense management and increased profits. The overall financial position of Atlas Battery appears stable based on favorable ratios such as return on equity, current ratio, and cash conversion cycle.
This document provides a summary of Mai Abd El-Monsef Sayed's professional experience and qualifications. She has over 10 years of experience in human resources management and is currently seeking a challenging career in the field. Her experience includes roles as an HR Manager, Senior Associate, and Generalist for various engineering, consulting, and food industry companies in Egypt and Kuwait. She has strong skills in recruitment, compensation, training, and performance management.
El documento discute el concepto de manifestaciones culturales. Explica que las manifestaciones culturales son actividades públicas y colectivas que producen un acto de comunicación para identificar a un grupo. También explica que una obra individual solo adquiere significado cultural cuando se hace pública y se comunica colectivamente. Además, describe brevemente algunas manifestaciones de la cultura peruana como Caral, Sechín, Chavín e Incas.
The resume summarizes Jay Prabhakaran's objective of working in a challenging environment to help achieve organizational goals. It provides details of his address, contact information, and dates of employment. Jay has over 10 years of experience in customer service and quality assurance roles for insurance companies. He seeks to leverage his analytical skills and certifications in Lean Six Sigma and quality auditing. His education includes an Insurance Foundation Certificate and Professional Certificate in Insurance from institutions in Ireland.
This document describes constructing deletion mutants of several genes in the uropathogenic E. coli strain UTI89. The key steps are:
1. PCR was used to generate gene disruption cassettes for spr, prc, eco293-sinI, and fimS.
2. The cassettes were electroporated into UTI89 cells expressing Red recombinase and plated on chloramphenicol to select mutants.
3. PCR confirmed successful disruption of the spr gene, replacing it with the cassette.
4. The cassette was then evicted by expressing FLP recombinase from pCP20, leaving only the gene deletion.
5. The process
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotesPsychoTech Services
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Our backs are like superheroes, holding us up and helping us move around. But sometimes, even superheroes can get hurt. That’s where slip discs come in.
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...Oleg Kshivets
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
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8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptxHolistified Wellness
We’re talking about Vedic Meditation, a form of meditation that has been around for at least 5,000 years. Back then, the people who lived in the Indus Valley, now known as India and Pakistan, practised meditation as a fundamental part of daily life. This knowledge that has given us yoga and Ayurveda, was known as Veda, hence the name Vedic. And though there are some written records, the practice has been passed down verbally from generation to generation.
- Video recording of this lecture in English language: https://youtu.be/Pt1nA32sdHQ
- Video recording of this lecture in Arabic language: https://youtu.be/uFdc9F0rlP0
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
1. 阿魏酸對人類肺癌細胞之抗腫瘤作用
Anti-tumor effect of ferulic acid on human lung cancer H1299 cells
長榮大學生物科技學系
陳宥安
實習單位:高雄醫學大學生物科技系
指導老師: 邱建智老師
Abstract
由過去研究發現,阿魏酸Ferulic acid (FA) 在藥理活性上具抗發炎、抑制血栓形成、抗自由基、抑制腫瘤細
胞生長及調節人體免疫功能的作用等等的活性。本論文主要以人類非小細胞肺癌細胞株作為研究對象,探討
FA引起H1299細胞生長及轉移的抑制能力,並藉由蛋白質表現的分析,探討其對肺癌細胞的作用機制。研究
結果顯示,FA轉移能力及抑制活性,與FA處理濃度和時間有關,在高濃度FA有明顯的生長抑制活性、轉移
能力也下降。利用PI的染色結果,FA能夠引起H1299細胞細胞週期停滯於G2/M期及誘導H1299細胞內ROS表
現下降,及不會導致細胞內粒線體膜電位下降。以明膠蛋白酵素電泳法 zymography assay進行肺癌細胞外培
養基的蛋白質分析,顯示FA處理後的 MMP-9及MMP-2 活性有明顯降低的趨勢。此外,以Migration assay及
Invasion assay進行FA對肺癌細胞作用的評估結果,也證實肺癌細胞在高濃度的轉移能力可被FA所抑制。綜合
上述結果,我們發現了FA萃取物與細胞轉移、移動能力有關,表示了天然植物所分離之物質FA在化學預防
___________________________________________________________________________
(chemoprevention)中有希望能成為癌症預防的新策略。
Experimental results FA
_________________________________
vehicle 0.03 0.06 0.15 0.3 0.6 (mM)
30
25
Denuded zone
Denude zone (%)
20
0h
15
10
14 h 5
0
con 0.03 0,06 0,15 0.3 0.6
Fig 4. NSCLC H1299 cells were incubated with a series of FA dose (mM)
different concentration of FA for 14hr to observation cells
Fig 1. Structure of Ferulic acid migration.
Vehicle 0.03 0.06 120
cell number
140 100
24 hr
invasion index (%)
48 hr
120 80
viable cell number (%)
100 60
80 40
60 20
40 0
0.15 0.3 0.6 (mM)
con 0.03 0.06 0.15 0.3 0.6
20 FA Dose (mM)
0 Fig 5. NSCLC H1299 cells were incubated with a series of different concentration
Control 0.03 0.06 0.15 0.3 0.6
FA Dose (mM)
of FA for 16hr to observation cells invasion.
Fig 2. NSCLC H1299 cells were incubated with a (mM)
series of different concentration of FA for 24 and Con 0.03 0.06 0.15 0.3 0.6
48 hr to observation cell viability.
_________________ FA
0 0.03 0.06 0.15 0.3 0.6 (mM)
MMP-2
MMP-9
120 120
100 100 Fig 6. NSCLC H1299 cells were incubated with a series of different concentration
Fold relative activity (%)
Flod relative activity (%)
80 80
of FA for 16hr to observation cell cycle distribution through flow cytometric
60 60
analysis.
40 40
20
0
20
0
Discussion
con 0.03 0.06 0.15 0.3 0.6 con 0.03 0.06 0.15 0.3 0.6
FA Dose (mM) FA Dose (mM)
1、The inhibitory effect of FA on migration of lung cancer cells,
Fig 3. NSCLC H1299 cells were incubated with a suggesting that FA could be a chemoprevention agent in the
series of different concentration of FA for 24hr after future。
take supernatant to observation matrix metallopro-
teinases (MMP-2 and MMP-9) activity.