24 - 29 June 2019. Hyderabad, India. The ANH Academy Week is a series of annual events that bring together the community of researchers and users of research (practitioners and policymakers) working at the intersection of agriculture, nutrition and health.

1. Amit K Barui, Balaji Srinivasan, David Erickson and Saurabh Mehta
Division of Nutritional Sciences,
Sibley School of Mechanical and Aerospace Engineering
Cornell University, Ithaca, NY
akb247@cornell.edu

2. SAFE-Phone
Smartphone based Aflatoxin Evaluation
• 4’ diagonal size, with a touch screen.
• Internet of Things operating system.
• Available to transmission data with Wi-Fi, Bluetooth, and 3G GSM
networks
• Confocal fluorescence and visible optical sensor.
• Excite fluorescence image and capture immunoassay signals.
• Ultra-compact reader
• Europium nanoparticle and Gold nanoparticle based lateral flow assay.
TIDBIT ver. #1
TIDBIT ver. #2

4. Aflatoxin B1 determination in peanut samples
0 156 312 625 1250 2500 5000
Aflatoxin B1 pg/mL
T
C
• Gold nanoparticle based lateral flow immunoassay.
• Assay covers the wide aflatoxin concentration range 300-5000 pg/mL.
• Limit of detection as low as 300 pg/mL
• Different batches of test strips were also analyzed to ascertain the inter
batch variability
Aflatoxin- absence
Aflatoxin- presence
Anti-aflatoxin gold nanoparticle conjugate Aflatoxin-BSA conjugate Anti-rat antibodyAflatoxin
T = Test line C = Control line
T C
T C
T C
T C
625 pg/mL 5000 pg/mL

5. Steps for aflatoxin B1 determination in peanut samples

6. Acknowledgements