AMiCI (CA15114). Workshop for Early Career Investigators (ECIs) and Short Term Scientific Missions (STSM) Riga, Latvia, 7th of March 2019

1. Antimicrobial Activity of
Electrospun Polylactic Acid
Nanofiber Composites
Dilayda KANMAZ1, Ozan TOPRAKCI1,
Hülya OLMEZ2, Christian ADLHART3
1: Yalova University, Faculty of Engineering, Department of Polymer Engineering, Yalova, Turkey
2: Biomaterials, Bioelectronics and Biomechanics (3B) Center of Excellence, Material Institute, TUBITAK
Marmara Research Center, Kocaeli, Turkey
3:Zurich University of Applied Sciences ZHAW, Institute of Chemistry and Biotechnology, Wädenswil,
Switzerland 1

2. • Wound Healing
• Wound Dressings
• Nanofiber Based Wound Dressings
• Research Highlights
• Production of Nanofiber Composites
• Morphology
• Antibacterial Activity Measurements
• Conclusions
2
Substance

3. Wound Healing
A: Hemostasis phase B: Inflammatory phase
C: Proliferation phase D: Maturation phase
3

4. Wound Dressings
• Composite dressings
• Transparent film dressings
• Hydrocolloids
• Alginate dressings
• Wound fillers
• Antibacterial dressings
• Hydrogel dressings
4

5. Nanofiber Based Wound Dressings
• Mimic the extracellular matrix structure
• High absorption capacity
• Protect the wound from bacterial penetration
and dehydration
• Migrate the oxygen
5

6. Research Highlights
• The objective: the fabrication of
phytochemical based antimicrobial nanofiber
composites via electrospinning
• Polylactic acid (PLA) used for the nanofiber
fabrication.
• Tannic acid used as antimicrobial agent.
• The antimicrobial activity of the composites
assessed against Escherichia coli.
6

7. Production of Nanofiber Composites
7

8. Solution Conductivity
8
Loading Ratios of
Antimicrobial
Agent
Conductivity
(µScm-1)
10% TA 13.32
20% TA 14.86

9. Morphology
9
0 50 100 150 200 250 300
0
10
20
30
40
50
Fiber Diameter Range (µm)
Frequency(%)
20%TA
0 50 100 150 200 250 300
0
10
20
30
40
50
60
70
80
Fiber Diameter Range (µm)
Frequency(%)
10%TA

10. Antibacterial Activity Measurements
10
PLA 10% TA 20% TA

11. Conclusions
• Nanofiber composites manufactured successfully.
• PLA nanofibers had random fiber orientation.
• The nanofiber diameters increased with increasing
TA amount.
• Conductivity increased with increasing TA amount.
• According to antibacterial activity tests, all the
tested samples showed a reduction in E.coli counts
ranging between 1-3 log counts (%90 to %99.9).
• The amount of TA in the PLA increased, the
antibacterial activity was also increased. 11

12. Acknowledgement
• Cost Action: CA 15114
• Anti-Microbial Coating Innovations to Prevent
Infectious Diseases (AMICI)
• Electrospun Polylactic Acid Based Nanofibers
for Biomedical Applications was published
between TUBITAK MRC and Yalova
University in journal of Material Science
Research India.
• Second joint publication between TUBITAK
MRC, Yalova University and Zurich University
will be the deliverable of this STSM. 12

13. Acknowledgement
• Thank you very much to Dear Sara MOUSAVI
for all her helps at Zurich University .
• Thank you very much to Dear Professor
Christian ADLHART.
• Thank you very much to Dear Professor Hülya
OLMEZ.
• Thank you very much to Dear Professor Ozan
TOPRAKCI.
• Thank you very much to my dear mother
Şebnem, my dear father Vedat, and my dear
friend Murat. 13

14. 14
Thank you very much
to all COST Action
family!