Sheffield Institute for Translation Neuroscience (SITraN)
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The document provides an overview of SITraN (Sheffield Institute for Translational Neuroscience), which focuses on translational neuroscience research. Specifically, SITraN is dedicated to studying motor neuron disease/amyotrophic lateral sclerosis (MND/ALS) as it shares similarities with other neurodegenerative diseases and is a good model for evaluating neuroprotective treatments. The document also describes the author's role working in SITraN's RNA Biology lab investigating RNA dysregulation in MND/ALS and Parkinson's disease, including their project on Chromosome 9 open reading frame 72 (C9orf72) mRNA in C9orf72-linked ALS.
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Sheffield Institute for Translation Neuroscience (SITraN)
- 2. Overview of SITraN • Focused on translational neuroscience • Findings from the lab Patients in clinic • Dedicated to Motor neurone disease/Amyotrophic lateral sclerosis because it: 1) shares similarities with other neurodegenerative diseases 2) makes a great model for evaluating all forms of neuroprotective treatment.
- 3. Overview of SITraN Epilepsy Stroke Parkinson’s disease Cognitive decline and dementia MND/ALS Spinal muscular atrophy • No cures • Most cases are late onset
- 4. Overview of SITraN • Numerous research teams • Multi-disciplinary • Collaborative environment • Working towards drug development • State-of-the-art facilities and technologies Zebrafish - GFP Motor neurons - stem cells
- 5. Work done/my role • RNA Biology lab • Research technician • Investigate RNA dysregulation • MND/ALS and Parkinson’s disease • My project • Chromosome 9 open reading frame 72 (C9orf72) mRNA in C9orf72-linkedALS. • Toxic proteins created?
- 6. My role • Experimental work • Tissue culture • Gel electrophoresis • Western blotting • Microbiological work • Polymerase chain reaction (PCR) • Responsibilities • Maintained stable cell lines • Stocked the lab • Mentored the MSc students • Public engagement work
- 7. Why I think it was good value • Gained a full year in a research lab • Expanded my network • Learned many techniques • Enhanced multiple skills • Advised to not do MSc, but apply for a PhD • Work I did is going into a paper • The research I was doing is aimed at saving lives in the future.
- 8. Acknowledgements • Dr Guillaume Hautbergue (My Principal Investigator and Head of RNA Biology lab) • Dr Lydia Castelli (Post-doctoral research associate and my day- to-day supervisor) • SITraN • Sheffield Hallam University • Sue Campbell –Visiting tutor