This study evaluated 59 orange-fleshed sweetpotato genotypes for drought tolerance using an in vitro screening technique with polyethylene glycol (PEG). The genotypes were grown in media with 0%, 10%, and 15% PEG to simulate drought stress. Results showed significant differences between genotypes and PEG levels for traits like root length, shoot growth, and dry weight. Ten genotypes performed best under drought stress and were identified as tolerant. The in vitro screening was found to effectively evaluate drought tolerance in a large number of genotypes in a short time compared to field trials. Some genotypes identified as tolerant through in vitro screening were later confirmed in field conditions as well.