This document discusses fluorescence and its applications in biology. It mentions Stokes experiment which discovered fluorescence using quinine and UV light. It also discusses green fluorescent protein from jellyfish, fluorescence resonance energy transfer (FRET) and its use in measuring molecular interactions through changes in fluorescence lifetime. FRET and fluorescence lifetime imaging microscopy (FLIM) provide evidence of energy transfer between molecules.

1. Fluorescence and Biology Sergi Padilla

2. George Gabriel Stokes Pink Floyd? microscope

3. Gin Tonic and Stokes Experiment U.V Light! Quinine found in tonic water !

5. FRET:

6. FRET and Lifetime:

7. Jelly fish glowing in the dark Biological light?

8. Green Fluorescent Protein out of Aquorea Victoria

9. Fluorescence decay: A population of a bunch of molecules relaxing

10.  t  D  DA Fluorescence decay of the donor FRETefficiency  t  D donor Proportion bound donor log I log I

12. Measurement of the mean lifetime:

13. . ACQUISITION BEFORE BLEACHING . ACQUISITION AFTER BLEACHING A Proof of FRET by FLIM

14. + + The Nobel prize in Chemistry 2008