This document describes the design and testing of a novel multi-epitope peptide vaccine against hepatocellular carcinoma (HCC). Bioinformatics tools were used to design a vaccine construct containing epitopes that could provoke both humoral and cellular immune responses. The vaccine protein was expressed in E. coli and its expression was confirmed using SDS-PAGE and Western blot analysis. The results showed that E. coli is an acceptable system for producing the recombinant multi-epitope peptide vaccine against HCC.

1. Estefanía Zapata Rúa
Tercer semestre
Universidad pontificia bolivariana

2. INTRODUCTION
Hepatocellular carcinoma (HCC) is the seventh most prevalent liver
cancer in the world and the fourth leading cause of cancer mortality
worldwide.
The carcinoma consists of epithelial cells, which are the cells that
line the inner and outer parts of the body.
Treatments against this type of cancer are scarce and it depends on
the stage of the cancer. Patients can be treated with surgery or
chemotherapy with sorafenib.

3. INTRODUCTION
Researches have used adoptive immunotherapy as indirect
immunological approaches including vaccines.
Vaccines are an effective strategy against tumor cells and are
generally divided into three types, including pulsed dendritic cells
(DCs), peptide-based vaccines, and DNA-based vaccines.
Multi-epitope peptide vaccines are used against pathogenic bacteria,
viruses, and tumor cells.

4. General Objetive
Production of a Novel Multi-Epitope Peptide
Vaccine against Hepatocellular Carcinoma

5. Designed Vaccine Construct
Expression of the Vaccine Protein
Materials and Methods
It was used bioinformatical servers (IEDB, ProPred-I
PAComplex and PAComplex ) for the design of a
novel multi-epitope peptide vaccine against HCC.
The media culture of E. coli was at 37° and 1 mL was diluted in
ampicillin. Then it was shaken induced via two different
concentrations of (IPTG). This process was to transform the cells
via centrifugation and the obtained pellets

6. Western Blot Test
SDS-PAGE Análisis
Materials and Methods
It was used to prove the expresión of the vaccine. This is a
technique used to identify a specific protein. The method
involves the use of gel electrophoresis to separate the proteins
in the sample.
It is used to separate proteins according to their
electrophoretic mobility. It is widely used to quantify,
compare and characterize proteins. Constitutes the
input technique for the performance of Western
Blotting

7. Results
The SDS-PAGE technique allowed to analyses of the
multi-epitope peptide
expression of the vaccine.
The result of SDS-PAGE analysis of the
multi-epitope peptide vaccine purification.

8. Results
The Western blot analysis results.

9. Author What the autor says Agree or
disagree
Sawada Y, Yoshikawa T,
Ofuji K, Yoshimura
M, Tsuchiya N, Takahashi M
The linear and conformational B-cell
epitopes and interferon-gamma-inducing
epitopes were recognized in the vaccine
construct, because provoking the humoral
and cellular immune responses is
essential in multiepitope peptide
vaccines.3
Agree
Li J, Xing Y, Zhou Z, Yao W,
Cao R, Li T
HCC is a cancer with a high mortality rate.
This malignancy is not usually diagnosed
in the primary stages, because it is known
as a heterogeneous disorder that includes
several etiological agents; therefore, it is
diagnosed in the advanced stages, when
it is usually late for treatment.
Agree
Gravel P, Golaz O. To prove the expression and purification
oft he recombinant vaccine protein, the
Western blot method was employed.
Agree
Discussions

10. Conclusions
E. coli is an acceptable organism to
represent recombinant proteins. This prokaryotic cell
allowed acceptable implementation techniques for
producing multi-epitope
peptide vaccine against HCC
Computer tools were of great help in creating a
novel multi-epitope peptide vaccine against HCC