Secondary Metabolism is a term for pathways for small molecule and products of metabolism that are not absolutely required for the survival of the organism.
A secondary metabolite has an important ecological function.
Examples include antibiotics, mycotoxins etc.
This document discusses biochemical defense mechanisms in plants. It describes pre-existing defenses like inhibitors released before pathogen penetration, lack of nutrients needed for pathogen growth, and absence of antigens shared by pathogen and host. Post-infection defenses are also summarized, including induced production of phenolic compounds and phytoalexins toxic to pathogens. The document explains how plants can produce substances to resist pathogen enzymes, detoxify pathogen toxins, and alter biochemistry to negatively impact pathogens through toxicity or hypersensitive responses.
Fungi produce a diverse array of secondary metabolites through several biosynthetic pathways. These metabolites serve various purposes in nature but also have applications in medicine and industry. The pathways that produce important fungal secondary metabolites involve polyketides from acetyl-CoA, the mevalonate pathway, and amino acids. Many fungal metabolites have complex chemical structures and are produced in clusters regulated by global genes. Examples of medically and industrially useful fungal metabolites include penicillin, aflatoxin, and ergot alkaloids.
The document discusses the defence mechanisms in plants against pathogens. It describes both structural and biochemical defences. Structural defences provide physical barriers like cuticles, cell walls and induced structures. Biochemical defences include pre-existing compounds like phenols, enzymes and toxins. Post-infection, plants produce phytoalexins, hypersensitivity response and new proteins to inhibit pathogens. The defences have evolved through co-evolution with pathogens and help plants survive attacks.
Secondary metabolites are substances produced by plants that are not directly involved in growth or development. They serve ecological functions like attracting insects for pollination or defending against herbivores and pathogens. Many secondary metabolites are toxic, such as proteins that impair digestion. Pathogenic microbes produce substances like enzymes and toxins to infect plants and cause disease. Plants have defenses like phytoalexins - antibiotic compounds synthesized in response to infection within hours. Phytoalexins come from groups like isoprenoids, flavonoids, and stilbenes. Plant defense is induced by elicitors, which are pathogen proteins that attack plants and wall fragments from the pathogen. Elicitors trigger signal cascades in the plant that activate gene
1. The document discusses primary and secondary metabolites in plants. Primary metabolites are directly involved in growth and development, while secondary metabolites are not essential for growth but play important roles in plant defense.
2. Carotenoids are secondary metabolites that serve as pigments in plants and protect against free radicals. They are involved in photosynthesis and attracting animals to disperse seeds.
3. Phenolic compounds form a diverse family of secondary metabolites that perform various functions for plants, including defense against herbivores and pathogens. They are produced through the shikimic acid and malonic acid pathways.
Secondary metabolites are organic compounds produced by organisms that are not essential for growth and development. They are derived from primary metabolites and provide functions like protection from predators and pathogens, attracting pollinators, and responding to environmental stresses. Some key points are:
- Secondary metabolites are more limited in distribution than primary metabolites and have no role in growth.
- They serve purposes like protecting plants from attacks and attracting pollinators rather than functions like growth and development.
- Major classes of secondary metabolites include alkaloids, glycosides, phenolics, and terpenoids which have a variety of chemical structures and biological activities.
This document discusses several types of secondary plant metabolites including phenolics, terpenoids, alkaloids, and others. Phenolics are derived from the shikimate pathway and include classes like phenols, hydroxybenzoates, flavonoids, and lignins. Terpenoids are made from the acetate-mevalonate pathway and include mono-, sesqui-, and diterpenes. Alkaloids contain nitrogen and can be toxic or used medicinally as in morphine, quinine, and caffeine. Secondary metabolites provide benefits to plants such as protection from predators and pathogens, attracting pollinators, and some have pharmaceutical applications.
Secondary Metabolism is a term for pathways for small molecule and products of metabolism that are not absolutely required for the survival of the organism.
A secondary metabolite has an important ecological function.
Examples include antibiotics, mycotoxins etc.
This document discusses biochemical defense mechanisms in plants. It describes pre-existing defenses like inhibitors released before pathogen penetration, lack of nutrients needed for pathogen growth, and absence of antigens shared by pathogen and host. Post-infection defenses are also summarized, including induced production of phenolic compounds and phytoalexins toxic to pathogens. The document explains how plants can produce substances to resist pathogen enzymes, detoxify pathogen toxins, and alter biochemistry to negatively impact pathogens through toxicity or hypersensitive responses.
Fungi produce a diverse array of secondary metabolites through several biosynthetic pathways. These metabolites serve various purposes in nature but also have applications in medicine and industry. The pathways that produce important fungal secondary metabolites involve polyketides from acetyl-CoA, the mevalonate pathway, and amino acids. Many fungal metabolites have complex chemical structures and are produced in clusters regulated by global genes. Examples of medically and industrially useful fungal metabolites include penicillin, aflatoxin, and ergot alkaloids.
The document discusses the defence mechanisms in plants against pathogens. It describes both structural and biochemical defences. Structural defences provide physical barriers like cuticles, cell walls and induced structures. Biochemical defences include pre-existing compounds like phenols, enzymes and toxins. Post-infection, plants produce phytoalexins, hypersensitivity response and new proteins to inhibit pathogens. The defences have evolved through co-evolution with pathogens and help plants survive attacks.
Secondary metabolites are substances produced by plants that are not directly involved in growth or development. They serve ecological functions like attracting insects for pollination or defending against herbivores and pathogens. Many secondary metabolites are toxic, such as proteins that impair digestion. Pathogenic microbes produce substances like enzymes and toxins to infect plants and cause disease. Plants have defenses like phytoalexins - antibiotic compounds synthesized in response to infection within hours. Phytoalexins come from groups like isoprenoids, flavonoids, and stilbenes. Plant defense is induced by elicitors, which are pathogen proteins that attack plants and wall fragments from the pathogen. Elicitors trigger signal cascades in the plant that activate gene
1. The document discusses primary and secondary metabolites in plants. Primary metabolites are directly involved in growth and development, while secondary metabolites are not essential for growth but play important roles in plant defense.
2. Carotenoids are secondary metabolites that serve as pigments in plants and protect against free radicals. They are involved in photosynthesis and attracting animals to disperse seeds.
3. Phenolic compounds form a diverse family of secondary metabolites that perform various functions for plants, including defense against herbivores and pathogens. They are produced through the shikimic acid and malonic acid pathways.
Secondary metabolites are organic compounds produced by organisms that are not essential for growth and development. They are derived from primary metabolites and provide functions like protection from predators and pathogens, attracting pollinators, and responding to environmental stresses. Some key points are:
- Secondary metabolites are more limited in distribution than primary metabolites and have no role in growth.
- They serve purposes like protecting plants from attacks and attracting pollinators rather than functions like growth and development.
- Major classes of secondary metabolites include alkaloids, glycosides, phenolics, and terpenoids which have a variety of chemical structures and biological activities.
This document discusses several types of secondary plant metabolites including phenolics, terpenoids, alkaloids, and others. Phenolics are derived from the shikimate pathway and include classes like phenols, hydroxybenzoates, flavonoids, and lignins. Terpenoids are made from the acetate-mevalonate pathway and include mono-, sesqui-, and diterpenes. Alkaloids contain nitrogen and can be toxic or used medicinally as in morphine, quinine, and caffeine. Secondary metabolites provide benefits to plants such as protection from predators and pathogens, attracting pollinators, and some have pharmaceutical applications.
This document summarizes the roles of enzymes, toxins, exopolysaccharides, and polypeptide signals in plant disease development. It discusses how enzymes secreted by pathogens break down plant cell walls and nutrients, allowing pathogens to utilize these resources. Different types of enzymes like hydrolases, hemicellulases, and proteolytic enzymes are described. Toxins directly act on and kill plant cells, causing disease symptoms. Exopolysaccharides protect bacterial biofilms and provide nutrients. Finally, the polypeptide signal systemin is discussed, which activates plant defense genes and functions long-distance within the plant in response to wounding or predator attacks.
The document summarizes pesticide metabolism in three phases. Phase 1 involves oxidation, reduction, and hydrolysis reactions mediated primarily by cytochrome P450 enzymes. Phase 2 involves conjugating pesticide metabolites to sugars, amino acids, and glutathione, increasing water solubility. Phase 3 further processes some phase 2 conjugates. The document also discusses pesticide bioremediation using microorganisms and enzymes, as well as the role of plant rhizospheres and chemical safeners in degradation.
Secondary metabolites are organic compounds produced by plants and organisms that are not essential for growth or reproduction. They play important roles in defense against herbivores and pathogens. Approximately 1500 new secondary metabolite molecules are identified from plants each year, around 30% of which show some biological activity. Secondary metabolites have many applications in medicine, food, cosmetics, and other industries. Plant tissue culture is used to produce many important secondary metabolites in a controlled environment, as production from native plants can be limited by environmental and geographical factors. Common production methods include cell suspension cultures, hairy root cultures, and immobilized cell cultures. Factors like media composition, temperature, pH, and elicitors can influence metabolite yield. An example is the production
Phytoalexins are broad-spectrum antimicrobial compounds produced by plants in response to pathogen infection. They are chemically diverse and fall into classes like terpenoids, steroids, and alkaloids. Phytoalexins are synthesized de novo from primary metabolites through pathways like the shikimic acid and mevalonic acid pathways after plants detect elicitors from pathogens. They function as toxins to disrupt the metabolism and reproduction of pathogens. Their production is part of plants' general short-term response to infection and their long-term systemic acquired resistance which protects the entire plant.
Primary metabolites are directly involved in normal growth, development and reproduction, and are essential for these processes. Examples include carbohydrates, proteins, lipids and nucleic acids. Secondary metabolites are not directly involved in these processes but have important ecological functions, such as antibiotics. Secondary metabolites include mycotoxins, antibiotics, alkaloids, amino acids, steroids and vitamins. They are produced late in the growth cycle and provide benefits to the producing organism or disadvantages to surrounding organisms.
Secondary plant metabolites are low molecular weight compounds produced in addition to primary metabolites. They provide protective functions for plants against pests and stress. Secondary metabolites include terpenes, phenolics, and nitrogen-containing compounds. Plants produce these chemicals through specialized metabolic pathways. Common secondary metabolites include alkaloids, flavonoids, glycosides, and terpenes. Plant cell and tissue culture techniques allow for the commercial production of valuable secondary metabolites like morphine, berberine, vinca alkaloids, and saffron compounds that are used in pharmaceuticals and food.
Secondary metabolites are low molecular weight compounds produced by plants in addition to primary metabolites. They include terpenes, phenolics, and nitrogen-containing compounds. Secondary metabolites provide benefits such as protecting against pests and attracting pollinators. They can be produced through plant cell and tissue cultures using bioreactors to provide medicines, flavors, food additives and other commercially important compounds in a more sustainable way than extracting from wild plants. Examples discussed include morphine, berberine, vinca alkaloids, ginseng, saffron, taxol and others.
This document discusses primary and secondary metabolites. It defines metabolites as intermediates and products of metabolism, typically small molecules with various functions. Primary metabolites are directly involved in growth, development and reproduction, while secondary metabolites are not directly involved in these processes but have important ecological functions like antibiotics. It provides examples of primary metabolites like carbohydrates, proteins and lipids. Secondary metabolites include antibiotics, mycotoxins, alkaloids, amino acids, steroids and vitamins. The document also discusses specific secondary metabolites like penicillin, cephalosporins, streptomycin and griseofulvin.
This document discusses primary and secondary metabolites. It defines metabolites as intermediates and products of metabolism, typically small molecules with various functions. Primary metabolites are directly involved in normal growth, development and reproduction, while secondary metabolites are not directly involved in these processes but have important ecological functions. Examples of primary metabolites include carbohydrates, proteins and lipids, while examples of secondary metabolites include antibiotics, mycotoxins, alkaloids, steroids and vitamins. The document provides details on the history and categories of both primary and secondary metabolites.
Primary metabolites are directly involved in normal growth, development and reproduction, and are essential for these processes. Examples include carbohydrates, proteins, lipids and nucleic acids. Secondary metabolites are not directly involved in these processes but have important ecological functions, such as antibiotics. Secondary metabolites are derived from primary metabolites but are synthesized later in the growth cycle. Examples of secondary metabolites discussed include antibiotics (penicillin, cephalosporins, streptomycin, griseofulvin), mycotoxins, alkaloids, steroids, vitamins and amino acids.
Insecticide may be defined as a substance or mixture of substances intended to kill, repel or otherwise prevent the insects.
Insecticides are the most powerful tools available for use in pest management. They are highly effective, rapid in curative action, adoptable to most situations, flexible in meeting changing agronomic and ecological conditions and economical
The document discusses various metabolic pathways in plants including primary and secondary metabolites. It describes the shikimic acid pathway and its role in synthesizing aromatic amino acids. The acetate/mevalonate pathway and its role in terpenoid biosynthesis is also covered. Various techniques used to study these pathways are outlined, including the use of radioactive isotopes as tracers to investigate biosynthesis through precursor-product relationships. The summary focuses on the key metabolic pathways and tracer techniques discussed in the document.
this presentation cover the topics of cell biotechnology and plant tissue culture. the basic terms used in plant cell culture are used and then different types of culture media and methods are discussed including cell suspension and callus culture,
The document discusses production of metabolites from plant cell cultures. It describes 7 aspects of the production process including selection of cell lines and analysis of secondary metabolites. Major advantages of cell cultures are production under controlled conditions and extraction of organic substances from callus cultures. Various techniques for secondary metabolite production are also outlined such as organ cultures, precursor addition, elicitation, hairy root cultures, and bioreactors. Examples of secondary metabolites produced include taxol, morphine, codeine, and berberine. Metabolic engineering approaches can improve productivity and decrease catabolism.
The document discusses primary and secondary metabolism in plants. Primary metabolism produces primary metabolites like carbohydrates, proteins, lipids and nucleic acids that are essential for growth and survival. The pathways for primary metabolism are essentially the same across organisms. Secondary metabolism produces secondary metabolites that are not essential for growth but provide functions like defense against predators through toxicity, attracting pollinators or dispersing seeds. Secondary metabolites have more limited distribution between taxa and their roles are often unclear.
Primary metabolites are essential compounds like carbohydrates, proteins, lipids and nucleic acids that are involved in fundamental processes like energy production and tissue formation in all organisms. Secondary metabolites are non-essential compounds produced by specialized metabolic pathways in only some organisms. They play roles in defense, adaptation and have pharmacological activities. Some major classes of secondary metabolites are terpenes, phenolics and nitrogen-containing compounds.
Different media are used to culture microorganisms and sterile technique is required to prevent contamination. Media and lab materials must be sterilized before use through autoclaving or pressure cooking. Microbes can be cultured in liquid tubes, solid slant tubes, or petri plates. Serial dilutions and plate counts are then used to estimate microbial populations from samples.
This document summarizes the roles of enzymes, toxins, exopolysaccharides, and polypeptide signals in plant disease development. It discusses how enzymes secreted by pathogens break down plant cell walls and nutrients, allowing pathogens to utilize these resources. Different types of enzymes like hydrolases, hemicellulases, and proteolytic enzymes are described. Toxins directly act on and kill plant cells, causing disease symptoms. Exopolysaccharides protect bacterial biofilms and provide nutrients. Finally, the polypeptide signal systemin is discussed, which activates plant defense genes and functions long-distance within the plant in response to wounding or predator attacks.
The document summarizes pesticide metabolism in three phases. Phase 1 involves oxidation, reduction, and hydrolysis reactions mediated primarily by cytochrome P450 enzymes. Phase 2 involves conjugating pesticide metabolites to sugars, amino acids, and glutathione, increasing water solubility. Phase 3 further processes some phase 2 conjugates. The document also discusses pesticide bioremediation using microorganisms and enzymes, as well as the role of plant rhizospheres and chemical safeners in degradation.
Secondary metabolites are organic compounds produced by plants and organisms that are not essential for growth or reproduction. They play important roles in defense against herbivores and pathogens. Approximately 1500 new secondary metabolite molecules are identified from plants each year, around 30% of which show some biological activity. Secondary metabolites have many applications in medicine, food, cosmetics, and other industries. Plant tissue culture is used to produce many important secondary metabolites in a controlled environment, as production from native plants can be limited by environmental and geographical factors. Common production methods include cell suspension cultures, hairy root cultures, and immobilized cell cultures. Factors like media composition, temperature, pH, and elicitors can influence metabolite yield. An example is the production
Phytoalexins are broad-spectrum antimicrobial compounds produced by plants in response to pathogen infection. They are chemically diverse and fall into classes like terpenoids, steroids, and alkaloids. Phytoalexins are synthesized de novo from primary metabolites through pathways like the shikimic acid and mevalonic acid pathways after plants detect elicitors from pathogens. They function as toxins to disrupt the metabolism and reproduction of pathogens. Their production is part of plants' general short-term response to infection and their long-term systemic acquired resistance which protects the entire plant.
Primary metabolites are directly involved in normal growth, development and reproduction, and are essential for these processes. Examples include carbohydrates, proteins, lipids and nucleic acids. Secondary metabolites are not directly involved in these processes but have important ecological functions, such as antibiotics. Secondary metabolites include mycotoxins, antibiotics, alkaloids, amino acids, steroids and vitamins. They are produced late in the growth cycle and provide benefits to the producing organism or disadvantages to surrounding organisms.
Secondary plant metabolites are low molecular weight compounds produced in addition to primary metabolites. They provide protective functions for plants against pests and stress. Secondary metabolites include terpenes, phenolics, and nitrogen-containing compounds. Plants produce these chemicals through specialized metabolic pathways. Common secondary metabolites include alkaloids, flavonoids, glycosides, and terpenes. Plant cell and tissue culture techniques allow for the commercial production of valuable secondary metabolites like morphine, berberine, vinca alkaloids, and saffron compounds that are used in pharmaceuticals and food.
Secondary metabolites are low molecular weight compounds produced by plants in addition to primary metabolites. They include terpenes, phenolics, and nitrogen-containing compounds. Secondary metabolites provide benefits such as protecting against pests and attracting pollinators. They can be produced through plant cell and tissue cultures using bioreactors to provide medicines, flavors, food additives and other commercially important compounds in a more sustainable way than extracting from wild plants. Examples discussed include morphine, berberine, vinca alkaloids, ginseng, saffron, taxol and others.
This document discusses primary and secondary metabolites. It defines metabolites as intermediates and products of metabolism, typically small molecules with various functions. Primary metabolites are directly involved in growth, development and reproduction, while secondary metabolites are not directly involved in these processes but have important ecological functions like antibiotics. It provides examples of primary metabolites like carbohydrates, proteins and lipids. Secondary metabolites include antibiotics, mycotoxins, alkaloids, amino acids, steroids and vitamins. The document also discusses specific secondary metabolites like penicillin, cephalosporins, streptomycin and griseofulvin.
This document discusses primary and secondary metabolites. It defines metabolites as intermediates and products of metabolism, typically small molecules with various functions. Primary metabolites are directly involved in normal growth, development and reproduction, while secondary metabolites are not directly involved in these processes but have important ecological functions. Examples of primary metabolites include carbohydrates, proteins and lipids, while examples of secondary metabolites include antibiotics, mycotoxins, alkaloids, steroids and vitamins. The document provides details on the history and categories of both primary and secondary metabolites.
Primary metabolites are directly involved in normal growth, development and reproduction, and are essential for these processes. Examples include carbohydrates, proteins, lipids and nucleic acids. Secondary metabolites are not directly involved in these processes but have important ecological functions, such as antibiotics. Secondary metabolites are derived from primary metabolites but are synthesized later in the growth cycle. Examples of secondary metabolites discussed include antibiotics (penicillin, cephalosporins, streptomycin, griseofulvin), mycotoxins, alkaloids, steroids, vitamins and amino acids.
Insecticide may be defined as a substance or mixture of substances intended to kill, repel or otherwise prevent the insects.
Insecticides are the most powerful tools available for use in pest management. They are highly effective, rapid in curative action, adoptable to most situations, flexible in meeting changing agronomic and ecological conditions and economical
The document discusses various metabolic pathways in plants including primary and secondary metabolites. It describes the shikimic acid pathway and its role in synthesizing aromatic amino acids. The acetate/mevalonate pathway and its role in terpenoid biosynthesis is also covered. Various techniques used to study these pathways are outlined, including the use of radioactive isotopes as tracers to investigate biosynthesis through precursor-product relationships. The summary focuses on the key metabolic pathways and tracer techniques discussed in the document.
this presentation cover the topics of cell biotechnology and plant tissue culture. the basic terms used in plant cell culture are used and then different types of culture media and methods are discussed including cell suspension and callus culture,
The document discusses production of metabolites from plant cell cultures. It describes 7 aspects of the production process including selection of cell lines and analysis of secondary metabolites. Major advantages of cell cultures are production under controlled conditions and extraction of organic substances from callus cultures. Various techniques for secondary metabolite production are also outlined such as organ cultures, precursor addition, elicitation, hairy root cultures, and bioreactors. Examples of secondary metabolites produced include taxol, morphine, codeine, and berberine. Metabolic engineering approaches can improve productivity and decrease catabolism.
The document discusses primary and secondary metabolism in plants. Primary metabolism produces primary metabolites like carbohydrates, proteins, lipids and nucleic acids that are essential for growth and survival. The pathways for primary metabolism are essentially the same across organisms. Secondary metabolism produces secondary metabolites that are not essential for growth but provide functions like defense against predators through toxicity, attracting pollinators or dispersing seeds. Secondary metabolites have more limited distribution between taxa and their roles are often unclear.
Primary metabolites are essential compounds like carbohydrates, proteins, lipids and nucleic acids that are involved in fundamental processes like energy production and tissue formation in all organisms. Secondary metabolites are non-essential compounds produced by specialized metabolic pathways in only some organisms. They play roles in defense, adaptation and have pharmacological activities. Some major classes of secondary metabolites are terpenes, phenolics and nitrogen-containing compounds.
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Different media are used to culture microorganisms and sterile technique is required to prevent contamination. Media and lab materials must be sterilized before use through autoclaving or pressure cooking. Microbes can be cultured in liquid tubes, solid slant tubes, or petri plates. Serial dilutions and plate counts are then used to estimate microbial populations from samples.
This document provides an overview of phytochrome, a photoreceptor pigment found in plants. It discusses the key points of phytochrome including its two forms (Pr and Pfr), its role in photomorphogenesis, discovery, biosynthesis, functions in processes like photoperiodism, and relationship to the circadian clock. The document also briefly mentions other plant photoreceptors like cryptochrome and their roles in light detection and responses. It provides definitions and explanations of technical terms in clear language.
This study evaluated the effects of various biofertilizer treatments on mulberry growth. Key findings:
1) Co-inoculation of potash mobilizing bacteria, phosphate solubilizing bacteria, and nitrogen fixing bacteria led to the highest growth, fresh leaf weight, root volume, organic carbon, and available P and K.
2) Treatments involving combinations of reduced (50-75%) inorganic fertilizers with biofertilizers still showed benefits like increased growth, nutrient levels, and soil properties over the control or full inorganic treatments alone.
3) Integrating biofertilizers with reduced chemical fertilizers has potential to improve crop productivity in a sustainable manner.
Micro- organisms transform organic matter into plant nutrients that are assimilated by plants. Soil organisms represent a large fraction of global terrestrial .
Micro- organisms transform organic matter into plant nutrients that are assimilated by plants. Soil organisms represent a large fraction of global terrestrial .
Micro- organisms transform organic matter into plant nutrients that are assimilated by plants. Soil organisms represent a large fraction of global terrestrial .
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2. Fungal secondary metabolites
• Secondary metabolites are compounds
produced by an organism that are not
required for primary metabolic processes.
Fungi produce an enormous array of
secondary metabolites, some of which are
important in industry
3. • Many fungi express secondary metabolites
that influence competitive outcomes.
• The compounds are expressed along with
enzymes necessary for extracellular digestion.
• The precise function of many of these
compounds in the natural environment,
however, is unclear.
4. • Secondary metabolites are generally
produced following active growth, and many
have an unusual chemical structure. Some
metabolites are found in a range of related
fungi, while others are only found in one or a
few species. The restricted distribution implies
a lack of general function of secondary
metabolites in fungi.
5. • six of the twenty most commonly prescribed
medications are of fungal origin. These
metabolites have been subjected to
combinatorial chemistry following growth in
selective media.
• Some metabolites are toxic to humans and
other animals. Yet others can modify the
growth and metabolism of plants.
6. The pathway
• Interestingly, the most important secondary
metabolites seem to be synthesised from one
or a combination of three biosynthetic
pathways: polyketides arising from Acetyl
Coenzyme A, mevalonate pathway that also
arises from Acetyl Coenzyme A, and from
amino acids.
7. • genes for the synthesis of some important
secondary metabolites are found clustered
together, and expression of the cluster
appears to be induced by one or a few global
regulators.
8. Polyketide Metabolites
• Polymerisation of acetate may result in the
formation of a fatty acid or a polyketide.
Polyketides result when a primer other than
acetate is included, and processing during
chain elongation results in the inclusion of
various other compounds. The chain may be
further processed by cyclisation, lactonisation,
or formation of thioesters or amides. The
result is a staggering number of possile
structures built from the simple primer units.
9. • Included among the polyketide secondary
metabolites are orsellinic acid,
tetrahydroxynaphthalene (precursor for
melanin), sterigmatocyctin, aflatoxins, statins,
and fumonisin.
10. Aflatoxin
• Aflatoxins are produced in members of the
Aspergillus parasiticus group via the
polyketide pathway. The pathway has around
20 steps, and the end products include a
diversity of related compounds
(bisfuranocoumarins) that can be readily
converted one to another.
11. • Aflatoxin B1 is one of the most toxic compounds
known. The toxin is formed commonly in plant
materials held at relatively high moisture and
temperature for long periods (ie growth in
tropics and sub‐tropics). Peanuts, corn and
cotton are readily contaminated in the field. The
Aspergillus parasiticus group of fungi are
common in soil. The fungi can colonise roots and
spread through the plant. When harvested,
contaminated seed will become toxic if not dried
immediately and held in a dry form.
13. • Aflatoxins are toxic and carcinogenic.
• The LSD 50 for ducks is 0.33 mg/kg. At lower
levels and following prolonged exposure, the
toxins cause liver cancer in humans.
• Aflatoxin B1 is converted to Aflatoxin M (in
milk) on passage through cows. Though less
toxic, it does illustrate the potential damage
caused by consumption of contaminated
product.
14. Aromatic Compounds
• Cyclic compounds can be synthesised via the
polyketide or shikimic acid pathways.
Zearalenone is one interesting example from
this group. The compound regulates
perithecium formation in the fungus. It also
has an oestrogenic effect in mammals.
16. Amino Acid Pathway
• Penicillin and cephalosporin are β lactam
antibiotics. β lactam antibiotics are produced
by a few Ascomycota and several bacteria.
• The precursors of these antibiotics are amino
acids. Synthesis of active antibiotics is
directed by the inclusion in the growth
medium of different organic and fatty acids
resulting in different side chains on the
compound.
17. • A second group of antibiotics derived from the
amino acid pathway are the defensins.
Defensins are peptides that act against
bacteria. They are found in animals where
they function to protect organs such as the
gingiva where bacterial densities are very
high. The first defensin found in fungi has
been called plectasin. The role of plectasin in
its host Pseudoplectania nigrella is unknown.
18. • Toxins derived from amino acid synthesis
include psilocybin (Psilocybe) and Bufotenine
(Amanita).
• These compounds act on nerve impulses,
resulting in hallucinations. The result is
thought to be due to the similarities between
the compounds and serotonin
19. Combination of Pathways
• Ergot alkaloids are synthesised from several
pathways. Trypotophan from the shikimic acid
pathway is attached to an isoprenoid moiety
from the mevalonate pathway, and several
amino acids from primary metabolism are added
depending on the final product. Ergot alkaloids
are produced as a complex mixture of related
compounds from a branched pathway.
• The activity of the alkaloids is as varied as the
compounds. In essence, the compounds may
function as vasodilators, hormone regulators,
and feeding deterrents. They can be active in
mammals and insects.