In this presentation during 4th National Conference Embriology ISAR, we discussed about the oocyte inefficiency faced by women, evaluated if there is any solution to overcome this problem, and finally suggested an alternative. All of this presenting evidence that the ovarian stimulation is not related to a decrease in embryo quality. Maximizing the number of oocytes retrieved during ovarian stimulation, is the best way to improve the cumulative live birth rates per ovarian stimulation, decreasing the number of ovarian stimulation and oocyte pick-ups necessary to achieve the mainly goal of an IVF treatment: a health live birth
Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy.
By Luis Alberto Velásquez Cumplido
Participation of the oviductal s100 calcium binding protein G in the genomic effect of estradiol that accelerates oviductal embryo transport in mated rats
Mariana Ríos1, Alexis Parada-Bustamante1, Luis A Velásquez2,3, Horacio B Croxatto2,3,4 and Pedro A Orihuela2,3*
By Luis Alberto Velasquez Cumplido
In this presentation during 4th National Conference Embriology ISAR, we discussed about the oocyte inefficiency faced by women, evaluated if there is any solution to overcome this problem, and finally suggested an alternative. All of this presenting evidence that the ovarian stimulation is not related to a decrease in embryo quality. Maximizing the number of oocytes retrieved during ovarian stimulation, is the best way to improve the cumulative live birth rates per ovarian stimulation, decreasing the number of ovarian stimulation and oocyte pick-ups necessary to achieve the mainly goal of an IVF treatment: a health live birth
Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy.
By Luis Alberto Velásquez Cumplido
Participation of the oviductal s100 calcium binding protein G in the genomic effect of estradiol that accelerates oviductal embryo transport in mated rats
Mariana Ríos1, Alexis Parada-Bustamante1, Luis A Velásquez2,3, Horacio B Croxatto2,3,4 and Pedro A Orihuela2,3*
By Luis Alberto Velasquez Cumplido
Freeze-all policy: systematic review and meta-analysisMatheus Roque
This study was presented during ASRM2018. This is a systematic review and meta-analysis evaluating the potential clinical, obstetrical and perinatal benefits of the freeze-all policy ver the fresh embryo transfer
IVF - Training in India -Dr.VM.Thomas,PhD,FSAB:chennai fertility center and r...Thomas Vadingadu
chennai fertility center and Research institute gives Intensive hands on training for Embryologist as well as for the Gynecologist.CFC trained more then 400 Doctors and embryologist world wide.CFC gives ongoing hands on training in all aspects of assisted reproductive technology and clinical embryology
For More Information
www.chennaifertilitycenter.com
www.ivftrainingindia.com
+919841165197
+919500084252
Luis Alberto Velasquez Cumplido
Grado académico: Doctor en Ciencias Biológicas, Mención Ciencias Fisiológicas
Institución: Pontificia Universidad Católica (PUC)
Fecha: 28 de Noviembre de 1997
LINEAS DE INVESTIGACIÓN
Mi foco de investigación se ha centrado en el uso de herramientas de biología molecular, celular y nanociencia para abordar problemas biomédicos básicos y aplicados. Mis proyectos básicos se centran en caracterizar las relaciones huésped hospero a nivel celular y molecular. Mis proyectos aplicados se centran en el uso de la nanopartículas para la liberación de compuestos con actividad biológica.
En los últimos 10 años he publicado 33 manuscritos en revistas con comité editorial, he participado en la presentación de 5 patentes y he presentado numerosos resúmenes en congresos nacionales e internacionales. Soy revisor de numerosas revistas nacionales e internacionales y mi laboratorio ha recibido numerosas distinciones a la excelencia científica.
Respecto a mis proyectos, soy director de Biomedicina del proyecto basal en Nanociencia y Nanotecnología CEDENNA. Me adjudique un proyecto a centros científicos de excelencia en la Wellcome trust, Inglaterra. Tengo un FONDECYT regular y un PBCT y soy subdirector de un proyecto INNOVA.
En mis proyectos colaboro con Robert Lange, del MIT, USA, con Andrew Sharkey de la Universidad de Cambridge y el Dr. Myron Cristodoulides de la Universidad de Southampton. En mi laboratorio he dirigido 14 tesis de pregrado, 4 de postgrado y un post-doctorado.
Title "In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer". This presentation was from reviewed journal that published on 2008.
In this presentation it was discussed the effects of controlled ovarian stimulation (COS) over the endometrium. Moreover, it was discussed the pros and cons of the freeze-all policy.
Freeze-all policy: systematic review and meta-analysisMatheus Roque
This study was presented during ASRM2018. This is a systematic review and meta-analysis evaluating the potential clinical, obstetrical and perinatal benefits of the freeze-all policy ver the fresh embryo transfer
IVF - Training in India -Dr.VM.Thomas,PhD,FSAB:chennai fertility center and r...Thomas Vadingadu
chennai fertility center and Research institute gives Intensive hands on training for Embryologist as well as for the Gynecologist.CFC trained more then 400 Doctors and embryologist world wide.CFC gives ongoing hands on training in all aspects of assisted reproductive technology and clinical embryology
For More Information
www.chennaifertilitycenter.com
www.ivftrainingindia.com
+919841165197
+919500084252
Luis Alberto Velasquez Cumplido
Grado académico: Doctor en Ciencias Biológicas, Mención Ciencias Fisiológicas
Institución: Pontificia Universidad Católica (PUC)
Fecha: 28 de Noviembre de 1997
LINEAS DE INVESTIGACIÓN
Mi foco de investigación se ha centrado en el uso de herramientas de biología molecular, celular y nanociencia para abordar problemas biomédicos básicos y aplicados. Mis proyectos básicos se centran en caracterizar las relaciones huésped hospero a nivel celular y molecular. Mis proyectos aplicados se centran en el uso de la nanopartículas para la liberación de compuestos con actividad biológica.
En los últimos 10 años he publicado 33 manuscritos en revistas con comité editorial, he participado en la presentación de 5 patentes y he presentado numerosos resúmenes en congresos nacionales e internacionales. Soy revisor de numerosas revistas nacionales e internacionales y mi laboratorio ha recibido numerosas distinciones a la excelencia científica.
Respecto a mis proyectos, soy director de Biomedicina del proyecto basal en Nanociencia y Nanotecnología CEDENNA. Me adjudique un proyecto a centros científicos de excelencia en la Wellcome trust, Inglaterra. Tengo un FONDECYT regular y un PBCT y soy subdirector de un proyecto INNOVA.
En mis proyectos colaboro con Robert Lange, del MIT, USA, con Andrew Sharkey de la Universidad de Cambridge y el Dr. Myron Cristodoulides de la Universidad de Southampton. En mi laboratorio he dirigido 14 tesis de pregrado, 4 de postgrado y un post-doctorado.
Title "In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer". This presentation was from reviewed journal that published on 2008.
In this presentation it was discussed the effects of controlled ovarian stimulation (COS) over the endometrium. Moreover, it was discussed the pros and cons of the freeze-all policy.
Level of 17-β Estradiol in follicular fluid for patient undergoes IVF as corr...iosrjce
IOSR Journal of Dental and Medical Sciences is one of the speciality Journal in Dental Science and Medical Science published by International Organization of Scientific Research (IOSR). The Journal publishes papers of the highest scientific merit and widest possible scope work in all areas related to medical and dental science. The Journal welcome review articles, leading medical and clinical research articles, technical notes, case reports and others.
Female fertility begins to decline many years prior to the onset of menopause despite continued regular ovulatory cycles. Although there is no strict definition of advanced reproductive age in women, infertility becomes more pronounced after the age of 35.
Mark Perloe, MD Atlanta, 404-843-2229 Learn about the factors that can adversely affect fertility and the tests that can help pinpoint problems. Fertility treatment options including IVF and other high tech options are presented.
Evaluation of Some Prominent Biochemical Agents in Menstrual Phases of Womeniosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Optimization of ovarian stimulation to improve success rate in ‘ART’Apollo Hospitals
ART is defined as the technique used where there is a need for in-vitro preparation or manipulation of gametes. The commonest ARTs are intrauterine insemination (IUI) and in-vitro fertilization (IVF). Ovarian stimulation is required with these procedures to increase the pregnancy rate as ART with natural cycle has a very low pregnancy rate. Optimizing pregnancy rates per cycle is the real basis for ovarian stimulation protocols in ART.
Optimization of ovarian stimulation to improve success rate in ‘ART’
SDBPoster4 (1)
1. #142 Age-specific changes in oogenesis & the sex peptide receptor in female Drosophila melanogaster
Margaret C. Bloch Qazi,1 Brian Hastings,1 and Claudia Fricke2
1. Gustavus Adolphus College, St. Peter, MN 56082 USA; and 2. Westfälische, Wilhelms-Universität, Münster, Germany
Introduction
Reproductive senescence is characterized as a decline in fertility with
increasing age. A need to better understand the nature and outcomes of
female reproductive senescence on gametogenesis and offspring
development is driven by an increasing awareness that females in natural
populations continue to reproduce as they age and that research on
oogenesis largely focuses on young animals1,2.We used the pomace fly,
Drosophila melanogaster,to explore effects of increasing female age on
oogenesis. Oogenesis is a continuous process in flies with various stages of
egg development occurring simultaneously.Declining fertility with
increasing female age is due, in part, to decreased germline stem cell (GSC)
activity, increased cell death, and changes in oocyte provisioning.3,4 The
relative effects of these processes and their overall contributions to the
progression of oogenesis with increasing female age have not been
described. Some female post-mating responses are mediated by the female
Sex Peptide Receptor (SPR).This receptor, expressed in the female
reproductive tract and CNS, binds the male seminal fluid peptide Sex
Peptide (SP).5 SPR promotes: GSC proliferation, sperm release from
storage, and oviposition.6,7,5 SPR’s role in other aspects of oogenesis is
unknown as is how this role may change with age. Older females show
decreased responsiveness to SP which might be due to changes in SPR
expression or function with increasing age.8
Methods
Results
Question: How do oogenesis and female responses
to male mating stimuli change as females age?
Predictions:
1. If decreasing germline stem cell activity and oocyte provisioning are major
factors in female reproductive senescence, then older females will have
lower ovariole activity.
2. If the female SPR regulates oogenesis, then the number of egg chambers in
young mated females > virgins inWT, but not SPR-/WT and/or SPR- females.
3. If SPR abundance or activity decreases with increasing age, then age-
related changes in ovariole activity of WT >WT/SPR- > SPR- females.
Conclusions
1. Older females have decreased ovariole activity by failing to maintain the
supply of new egg chambers and vitellogenesis.This is consistent with
earlier work showing a decrease in GSC proliferation and protein
homeostasis in older females.
2. In young females, SPR does not appear to have a major influence on
vitellogenesis, but does stimulate release of mature oocytes from the
ovaries reflecting SP-SPR’s role in increased egg laying after mating.
3. SPR mediates some aspects of female oogenesis. SPR promotes
vitellogenesis in older females.This may be due to decreasing influence of
other mechanisms regulating oogenesis or other potential roles of SPR
other than as an SP receptor.
These experiments do not support a role of decreased levels/sensitivity of
SPR in the age-related decline in SPs’ effects on females. These experiments
demonstrate that aging is a dynamic condition that affects multiple aspects of
development and, in turn, is mediated by multiple mechanisms.
Acknowledgements
MBQ and BH thank the FYRE program at Gustavus Adolphus College for summer support. CF was supported by
DFG. Dolors Amoros-Moya provided support with experiments.
Literature Cited
(1) Nussey et al. 2013. Ageing Res Rev. 12:214-225. (2) Miller et al. 2014. Fly 8(3):1-13. (3) Zhao et al. 2008.Aging
Cell. 7:344-354. (4) Fredriksson et al. 2012.Aging Cell. 11:634-643. (5)Yapici et al. 2008. Nature. 451:33-36. (6)
Ameku & Niwa. 2016. PLoS Genet. 12(6): e1006123. (7) Avila et al. 2015. J Insect Physiol. 76:1-6. (8) Fricke et al.
2013. Proc R Soc B 280: 20130428. (9) Cummings & King. 1969. J. Morph.128(4): 427-442. (10) Image from: Miller.
1950. Biology of Drosophila. Cold Spring Harbor Laboratory Press. Cold Spring Harbor: NY.
Pre-vitellogenic
(stages 1-6)
Vitellogenic
(stages 7-12)
Post-vitellogenic
(stages 13 & 14)
1. Older females have lower ovariole activity in
pre-vitellogenic and vitellogenic stages
II. In young females, SPR promotes egg laying
not oogenesis
III. In old females, SPR supports vitellogenesis
2
4
6
8
virgin mated virgin mated virgin mated
Mean
#
egg
chambers/ovariole
0
0.5
1.0
1.5
2.0
2.5
3d 32d 3d 32d 3d 32d
Mean
(+1
SE)
#
vitellogenicegg
chambers/ovariole
↓
12.3%
n=61,
p=0.149
↓
19.9%
n=80,
p<0.0005
↓
41.9%
n=92,
p=0.005
Experimental Females
• WT = Dahomey strain
• SPR-/WT = Df(1)Exel6234/Dahomey white strain*
• SPR- = Homozygous Df(1)Exel6234+
* Dahomey strain backcrossed into the white (w1118) strain for 6 generations.
+ derived from Bloomington line #7708 backcrossed into Dahomey strain for 6 generations.
Female (3d or 32d old)
virgin
or
mated
(w/ Dahomey 3d old male)
WT
WT
SPR-/WT
SPR-/WT
SPR-
SPR-
Dissect, count ovarioles & stage
egg chambers (st 1-14)9,10
3d 32d 3d 32d
WT SPR-/WT
1
2
3
4
5
6
7
0
Mean
#
egg
chambers/ovariole
Ovariole number does not differ by mating status or age
An age-related decline in ovariole activity is not due to differences in the
number of ovarioles (age F1,209=0.075, p=0.784). Ovariole number differs by
genotype, but not SPR status (F2,209=8.39, p<0.0005):WT females had
significantly fewer ovarioles (p<0.001) than SPR- and SPR-/WT females (nd).
Pre-‐
Vitello.-‐
Post-‐
Total: F1,53=0.001, p=0.972
Post: F1,53=2.07, p=0.157
Vitello.: F1,53=1.70, p=0.198
Pre: F1,53=1.86, p=0.179
F1,72=27.76, p<0.0005
F1,72=2.07, p=0.155
F1,72=29.66, p<0.0005
F1,72=28.43, p<0.0005
n=40
n=40
n=31 n=30
Age: F1,209=9.72,p<0.0005
24h or 96h
0
Pre-‐
Vitel.-‐
Post-‐*
Post-vitellogenic egg chambers of 3d old females 24h post mating
mating status x SPR status: F2,51=36.99,p<0.0005
t21=2.24
p=0.038
*t4.68=5.63
p=0.003
t18=0.15
p=0.886
There was no difference among genotypes in the number of pre-vitellogenic or
vitellogenic egg chambers.