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Scheme carbohydrates
UNKNOWN CARBOHYDRATE SOLUTION
Dr Rohini C Sane
Professor
Department of Biochemistry
DY PATIL MEDICAL COLLEGE ,EBENE
UNKNOWN CARBOHYDRATE SOLUTION
1.BENEDICT’S TEST
POSITIVE NEGATIVE
REDUCING CARBOHYDRATE NON REDUCING CARBOHYDRATE
GLUCOSE, FRUCTOSE,LACTOSE ,MALTOSE SUCROSE
2. BARFOED’S TEST 2. BARFOED’S TEST
POSITIVE NEGATIVE NEGATIVE
MONOSACCHARIDE DISACCHARIDE DISACCHARIDE
GLUCOSE, FRUCTOSE LACTOSE ,MALTOSE
3. SELIWANOFF TEST 3. SELIWANOFF TEST 3. SELIWANOFF TEST
POSITIVE NEGATIVE NEGATIVE POSITIVE
FRUCTOSE GLUCOSE LACTOSE MALTOSE SUCROSE
OSAZONES –BUNDLES OF HAY LADY POWDER PUFF SUNFLOWER SHAPED CT INVERSION TEST
GLUCOSE FRUCTOSE LACTOSE MALTOSE SUCROSE
BENEDICT”S
TEST
POSITIVE POSITIVE POSITIVE POSITIVE NEGATIVE
BARFOED’s
TEST
POSITIVE POSITIVE NEGATIVE NEGATIVE NEGATIVE
SELIWANOFF ‘S
TEST
NEGATIVE POSITIVE NEGATIVE NEGATIVE POSITIVE
OSAZONES HAY SHAPED
(GRASS)
,GREEN
HAY SHAPED
(GRASS)
,GREEN
LADY POWDER
PUFF
SUNFLOWER
SHAPED
NO OSAZONES
CONCLUSIONS REDUCING
MONOSACCHARIDE
,ALDOSE
REDUCING
MONOSACCHARIDE
,KETOSE
REDUCING
DISACCHARIDE
,ALDOSE
REDUCING
DISACCHARIDE
,ALDOSE
NON REDUCING
DISACCHARIDE
,KETOSE
Carbohydrate Identification Tests Guide
Carbohydrate Identification Tests Guide
Carbohydrate Identification Tests Guide
Carbohydrate Identification Tests Guide

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Carbohydrate Identification Tests Guide

  • 1. Scheme carbohydrates UNKNOWN CARBOHYDRATE SOLUTION Dr Rohini C Sane Professor Department of Biochemistry DY PATIL MEDICAL COLLEGE ,EBENE
  • 2. UNKNOWN CARBOHYDRATE SOLUTION 1.BENEDICT’S TEST POSITIVE NEGATIVE REDUCING CARBOHYDRATE NON REDUCING CARBOHYDRATE GLUCOSE, FRUCTOSE,LACTOSE ,MALTOSE SUCROSE 2. BARFOED’S TEST 2. BARFOED’S TEST POSITIVE NEGATIVE NEGATIVE MONOSACCHARIDE DISACCHARIDE DISACCHARIDE GLUCOSE, FRUCTOSE LACTOSE ,MALTOSE 3. SELIWANOFF TEST 3. SELIWANOFF TEST 3. SELIWANOFF TEST POSITIVE NEGATIVE NEGATIVE POSITIVE FRUCTOSE GLUCOSE LACTOSE MALTOSE SUCROSE OSAZONES –BUNDLES OF HAY LADY POWDER PUFF SUNFLOWER SHAPED CT INVERSION TEST
  • 3. GLUCOSE FRUCTOSE LACTOSE MALTOSE SUCROSE BENEDICT”S TEST POSITIVE POSITIVE POSITIVE POSITIVE NEGATIVE BARFOED’s TEST POSITIVE POSITIVE NEGATIVE NEGATIVE NEGATIVE SELIWANOFF ‘S TEST NEGATIVE POSITIVE NEGATIVE NEGATIVE POSITIVE OSAZONES HAY SHAPED (GRASS) ,GREEN HAY SHAPED (GRASS) ,GREEN LADY POWDER PUFF SUNFLOWER SHAPED NO OSAZONES CONCLUSIONS REDUCING MONOSACCHARIDE ,ALDOSE REDUCING MONOSACCHARIDE ,KETOSE REDUCING DISACCHARIDE ,ALDOSE REDUCING DISACCHARIDE ,ALDOSE NON REDUCING DISACCHARIDE ,KETOSE