Benedict's reagent is used to test for reducing sugars like glucose, lactose, and fructose. It contains copper sulfate that is reduced to copper oxide when heated with reducing sugars, causing a color change from blue to green, brick red, or brown. Benedict's reagent can also detect glucose in urine samples to test for diabetes. Barfoed's reagent is similar but uses acetic acid, allowing it to distinguish between mono- and disaccharides. Disaccharides do not react for up to 10 minutes while monosaccharides form a blue or green precipitate. Sucrose is hydrolyzed using acid or enzyme to form glucose and fructose in the inversion test.

1. The characterization of Carbohydrates CHEM 5 - BSN

16. Hydrolysis of Sucrose