The document summarizes key findings from experiments comparing the bacterial enzyme RNA polymerase (RNAP) from E. coli and the thermophilic bacterium Thermus aquaticus (Taq). It was found that while Taq RNAP required 100 times higher concentrations of the antibiotic rifampicin (Rif) to inhibit transcription compared to E. coli RNAP, the two enzymes bind Rif in the same way and Rif inhibits transcription through the same mechanism. Additionally, experiments using chimeric Rif-nucleotide compounds showed that the disposition of the Rif binding site relative to the active site is identical between the two RNAPs. Therefore, Taq RNAP can serve as a model for studying Rif interactions with other RNAPs