The document summarizes the production of a single chain antibody fragment (scFabuv) that binds to green fluorescent protein (GFPuv) in the yeast Pichia pastoris. Key steps included amplifying the light and heavy antibody chains separately with PCR, linking them together with a linker, inserting this fusion into the pPICZalphaA plasmid, transforming it into P. pastoris, selecting clones, and inducing clones to secrete the scFabuv fragment into the media. Analysis with SDS-PAGE and western blot showed that several clones successfully secreted the scFabuv protein after induction. Future work will test the binding activity of the secreted scFabuv to its target GFPuv.