3. Introduction
• Tissue culture
The process of growing cells artificially in the laboratory
• Plant tissue culture
The technique in which plant tissues are places in a suitable medium the
tissue develop in to small plants and then new plants are sown in soil
where they grow.
4. Explant
• Any part of plant that take out and grown in Petry plate under sterile
conditions in special nutrient media is known as explant.
• Types of explant
Single cell culture
Shoot and root culture
Meristem culture
6. Environmental conditions
• Three important aspects in Invitro culture
1. Nutrient medium
2. Aseptic conditions
3. Aeration of the tissue
7. Advantages
• Large number of plants can be grown in short time
• Disease free plants can be grown
• Vegetative parts of the parent plant like stem ,root, leaves can be taken
to grow new plant
• Improved verities of plants can be produced.
8. Plants Grown in tissue culture
• Rubber tree
• Cassava
• Sweet potato
• Banana
• Date
