Rima Hadjouti et al (2022) Phytochemical content and foreign rhizobacteria. 7 Slide Presentation with oral commentary.pptx
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The document examines the phytochemical content of zucchini (Cucurbita pepo L.) treated with rhizobacteria isolated from olive trees (Olea europaea L.). Eleven bacterial strains were tested for their ability to promote plant growth and influence phytochemical parameters in zucchini. The bacteria significantly increased zucchini growth factors like root length, number of leaves and flowers. They also enhanced phytochemical contents such as flower pigmentation and polyphenol levels compared to untreated controls. The study demonstrates the potential of using rhizobacteria to improve crop nutrition and phytochemical production through plant-microbe interactions in the rhizosphere.
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Rima Hadjouti et al (2022) Phytochemical content and foreign rhizobacteria. 7 Slide Presentation with oral commentary.pptx
- 1. Phytochemical content of Cucurbita pepo L. and foreign rhizobacterial complements Rima Hadjouti1, Hakima M. Kaci1, Farida Benzina1, Malika Boudani2, Karima Aili2, Sid Ali Saadi3 , James N. Furze4, 5 1 Laboratoire de Valorisation et Conservation des Ressources Biologiques (VALCOR), Université M'hamed Bougara, Algeria. 2 Faculté des Sciences, Université M'hamed Bougara, Algeria. 3 Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algeria. 4 Laboratory of Biotechnology and Valorization of Natural Resources, Ibn Zohr University, Morocco. 5 Control and Systems Engineering Department, University of Technology-Iraq, Iraq
- 2. Brachypodium Root exudates Organic acids Small molecules Hormones Competition / Colonization Recognition / Recruitment Chemical signalling Rhizosphere Rhizospheric microbiome Bulk soil microbiome Soil environment Geography Climate Soil type Moisture conditions Terrestrial systems functioning Edaphic microorganisms Plant biomass production Rhizosphere Soil Fig. 1 Edaphic micro-organisms, the keys actors in plant biomass production Fig. 2 Rhizospheric components and processes (White et al. 2017)
- 3. Fig. 3 Plant growth promoting rhizobacteria (PGPR) are a natural alternative to chemical intervention in the soil (GHG – greenhouse gases) Sustainable agriculture PGPR Biotechnology Nutrient availability and uptake; stress tolerance plant / receptor stimulation Decrease fertility and soil health Increase salinity / acidity Harden the soil Destroy soil organisms / organic content Pollute air water and release GHGs Affect food quality and taste Chemical fertilizers
- 4. Methods/ approach PGP molecules: IAA; Siderophores, Phosphatase, Ammonia CWD Enzymes: Lipase, Phospholipase, Glucanase Germination and vegetative parameters Phytochemical parameters Fig. 4 Rhizobacteria from Olea europaea: a) Investigating plant growth promoting (PGP) and cell wall degrading enzyme (CWDE) activity, IAA –Indole-3-acetic acid; b) In vivo assays on zucchini and effects on phytochemical parameters a b
- 5. 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 Root length (cm) Strain 0 5 10 15 20 25 Control B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 Number of lateral roots Strain 0 2 4 6 8 10 12 14 16 18 Number of leaves Strain 0 2 4 6 8 10 12 14 16 Control B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 Number of flowers Strain 0 1 2 3 4 5 6 Control B1 B2 B3 B4 B5 B7 B9 B10 B11 Flower pigment (µg/mg) Strain Chlorophyll A Chlorophyll B Chlorophyll A+B Carotenoid 0 0.2 0.4 0.6 0.8 1 1.2 Control B1 B2 B3 B7 B9 B10 B11 Total polyphenols (mg/mL) Strain Fig. 6 Bacterial effects on phytochemical parameters of zucchini: a) flower pigmentation; b) total polyphenol Fig. 5 Vegetative growth assays on zucchini culture after 52 days: a) root length; b) number of lateral roots; c) number of leaves; d) number of flowers (Hadjouti et al. 2022) b a a b d c
- 6. Fig. 8 Visible effect of bacterial strains on zucchini growth Fig. 7 Rhizospheric bacteria (PGPR) plant interactions (Oulebsir-Mohandkaci et al. 2021; Shah et al. 2021) Control Treated zuchinni Bacteria Fungi Virus Nematodes and insects Drought Temperature Salinity Acidity Nitrogen fixation Nutrient uptake P-solubalization K-solubalization Phytohormones Siderophore Biocontrol Induced systemic acquired resistance PGPR Biotic Stresses Abiotic Stresses Nutrient availabilit y and uptake Enhanced stress tolerance
- 7. Acknowledgements We acknowledge the Algerian Ministry of Higher Education (DGRST), and PRFU project program (Code: D00L05UN350120180004) for support. We gratefully acknowledge the Algerian National Centre for Seed and Plant Control and Certification for supplying zucchini seeds. ----------------------------------------------------------------------------------------------------------------------------------------------------------------- References • Hadjouti R, Mohandkaci H, Benzina F et al (2022) Enhancing agriculture recovery of Phaseolus vulgaris L. and Cucurbita pepo L. with Olea europaea L. plant growth-promoting rhizobacteria. Soil Res. https://doi:10.1071/SR21320 • Oulebsir-Mohandkaci H, Benzina-Tihar F, Hadjouti R (2021) Exploring biofertilizer potential of plant growth-promoting rhizobacteria Bacillus clausii strain B8 (MT305787) on Brassica napus and Medicago sativa. Not Bot Horti Agrobot Cluj Napoca 49(4):12484-12484. https://doi.org/10.15835/nbha49412484 • Shah A, Nazari M, Antar M et al (2021) PGPR in agriculture: a sustainable approach to increasing climate change resilience. Front Sustain Food Syst. https://doi.org/10.3389/fsufs.2021.667546 • White IIIRA, Rivas-Ubach A, Borkum MI et al (2017) The state of rhizospheric science in the era of multi-omics: a practical guide to omics technologies. Rhizosphere 3:212-221. https://doi.org/10.1016/j.rhisph. 2017.05.003
Editor's Notes
- Soil upholds the functioning of terrestrial systems. Attributes include water, climate-control, nutrient cycling and biodiversity. Soil is a vulnerable, limited resource; producing food, energy and raw materials. Soils provide ecosystem functions for human well-being; supporting plant biomass production. Edaphic rhizospheres are rich in microorganisms. Microbial functional-diversity influences soil processes and functions.
- Plant growth promoting rhizobacteria (PGPR) are a group of bacteria inhabiting plant rhizospheres, and enable wide and ecologically significant functions. PGPR-based biotechnology elicits soil-inoculant developments, contributing to sustainable agriculture; reducing the need for chemical intervention.
- Rhizobacteria from Olea europea were tested for their capacity to produce plant growth promoting (PGP) molecules in vitro including Indole acetic acid, siderophores, phosphatase and ammonia; cell-wall degrading enzymes: lipase, phospholipase and glucanase. Additonal in vivo tests on zucchini seeds, germination and vegetative parameters were performed and subjected to statistical analysis. Phytochemical parameters of zucchini plants were measured, quantifying the water content of fresh-leaves, mineral content of aerial parts, presence of different pigments of the leaves and determination of total polyphenol content.
- The tested rhizobacteria showed a capacity to produce PGP molecules and cell-wall degrading enzymes. They showed an ability to significantly enhance root length, lateral root formation, the number of leaves and flowers. Further, rhizobacteria enhanced carotenoid, chlorophyll A and total polyphenol content.
- The soil environment has ecological niches endowed with diverse chemical processes. Rhizobacteria with PGPR molecules resolve soil deficits including provision of biochemical resources for plant health and development, leading to optimisation of agro-ecological food production for human and ecosystem benefit.