This document is a resume for Patricia Mantero. It summarizes her education, research experience, laboratory skills, teaching experience, honors and awards, languages, and computer skills. She has a Bachelor's degree in Biology from Penn State University and seeks a Master's degree in Biomedical Sciences. Her research experience includes laboratory technician work and undergraduate research studying gene expression, immune responses, and parasitic infections. She has skills in microbiology, molecular biology, and general laboratory techniques.

1. Patricia Mantero
55 Rosedale Apartments (939)-389-3966
Hershey, PA 17033 patriciamantero7@gmail.com
Objective:
To pursue a Master’s degree in Biomedical Sciences
Education:
May 2015– Bachelor of General Biology
Department of Biology, Pennsylvania StateUniversity
(Freshman/Sophomore, PSU-Altoona & Junior/Senior, PSU-University Park)
May 2011– High School Diploma
I graduated from Academia del Perpetuo Socorro; San Juan, PR
Research Experience:
November 2015-Present: Laboratory Technician I, Drug Discovery Development & Delivery Core
Facility
Laboratory of Dr. Kent Vrana, Department of Pharmacology, College of Medicine, Hershey, PA
Research Objectives: Provided technical support for a variety of drug screening assays (five different clients)
June 2015-November 2015: Intern, Genomic Sciences Core Facility
Laboratory of Dr. Yuka Imamura, Department of Biochemistry, College of Medicine, Hershey, PA
Research Objectives: Conducted nucleic acid isolation, quality assurance and characterization, sequencing DNA
library construction, and initiation of next-generation sequencing.
September 2014–May 2015: Undergraduate Research
Laboratory of Dr. Tim Miyashiro, Department of Biochemistry and Molecular Biology, Pennsylvania State
University, State College, PA
Research Objectives: Study of the mechanism of gene expression in a symbiotic relationship between the
bioluminescent bacterium, Vibrio fischeri, and the Hawaiian squid, Euprymna scolopes.
August to January 2014: Undergraduate Research
Laboratoryof Dr. Tracy Langkilde, Department of Biology, Pennsylvania State University, State College, PA
Research Objectives: Characterization of immune responses of Eastern fence lizards (Sceloporus undulatus)
after repeated acute elevation of corticosterone; primarily involved in observing videos of the lizard’s behavior
when fire ants attacked them.
January 2012 to May 2013: Undergraduate Research
Laboratory of Dr. Edward Levri, Biology Department, Pennsylvania State University, Altoona, PA
Research Objectives: Studied the influence of trematode parasitic infection in gastropod mollusks.
Primarily involved in: animal care (feeding, sterilizing tanks, observing behavior); prep samples and measure
samples, observe morphological structures to determine several physiological characteristics.

2. Laboratory Skills:
General Laboratory Practices:
 Data collection and documentation in lab notebook and scientific communication
 Handling and disposal of hazardous waste
 Annual lab safety training and laboratory animal training
 Computer programs to record and process data (Word, Excel, PubMed, Prism)
Solution and Media Preparation
 Use of micropipettes (including cleaning and calibration)
 Preparation of molal, molar and percent solutions
 Measure pH of solutions
 Filter and sterilize solutions
 Gel Gradient Preparation
Microbiology Techniques
 Isolate and grow bacterial cultures
 Perform the blue/white screening and antibiotic screening
 Measure the size of the colonies under different culture conditions
 Make LB agar plates; cell culture preparation
Other related lab techniques
 Observe whole animals under microscope to identify morphology
 Maintain cultures of mollusks
 Dissect invertebrates to observe and collect specific tissues.
Laboratory Instruments and Techniques
 Quantitative Analysis using a UV-Vis Spectrophotometer
 Use, cleaning and/or calibration of balances, centrifuges, and routine laboratory equipment
 Thin Layer Chromatography (TLC); Column Chromatography
 Organic extraction; simple and fractional distillation
 Use of light compound microscope, stereomicroscope
 DNA extraction from aragose gel; Western Blot; Quality check of samples in BioAnalyzer (High
Sensitivity DNA & RNA), TapeStation, Qubit, Nanodrop and Picogreen assay
 CASAVA demultiplexing of sequencing data
 mirVana RNA extraction
 Nextera library preparation for single cells cDNA
 Isolate DNA from FFPE tissues samples using Kapa Hyperplus reagents
 Isolation of RNA in blood samples and tissues

3. Teaching Experience:
January to May 2013 - Penn State Altoona- Spanish Tutor
Volunteer tutoring a group of college students
Honors and Awards
2012 -2013 Intercollegiate Athletics (Track & Field; Cross Country, Division III), Pennsylvania State
University, Altoona, PA
2012-2013 Academic All-Conference – This award is given to the athletes’ students who maintained a
GPA above 3.0
2012-2013 Conference New Comer of the Year – This award is given to the newcomer athletes to
recognize their achievement
2012-2013 All conference – This award is given to the athletes that were in the top 20 throughout the
whole season
Languages:
 Fluent in Spanish and English.
Computer Skills:
 Computer skills using all Microsoft Office software (Power Point, Excel, Microsoft Word)
 Prism Software
 R console
Posters and Oral Presentations
The Effect of Light Intensity on the Photokinetic Behavior of the New Zealand Mud Snail (Potamopyrgus antipodarum). Edward
P. Levri, Patricia Mantero, Sarah Landis, Brittany Smith, Elissa Colledge, and Alyssa Byrd. Penn State Altoona,
Altoona, PA 16601.
Yuka Imamura, Robert M. Brucklacher, Georgina V. Bixler, Elizabeth Conroy, Anna Salzberg, Susan D.
Patrick, Patricia Mantero, Kent E.Vrana, James R. broach. Institute for Personalized Medicine, Department of
Pharmacology and Biochemistry and Molecular Biology, Penn State College of Medicine (poster presentation).
4th
Annual Biochemistry and Molecular Biology Retreat, Sept 2015, Hershey, PA.
Publications:
Vrana KE, Rinnen TL, Rowe IV JC, Mantero P, Beck AJ, Karpa KJ, and Carkaci-Salli N. OFF-TARGET
INHIBITION OF TRYPTOPHAN HYDROXYLASE BY FDA-APPROVED DRUGS. (2016)
Pharmacology; in preparation.