1. Dietary Modifications of AGE Products in
Non Alcoholic Fatty Liver Disease
Management
Evangelos Matselis (3508326)
Pulasthi Gunasekara (3492351)

2. PROJECT OVERVIEW
Research Question:
Low-AGE’s diet is expected to improve patient’s health while High-AGE’s
diet is expected worsen patient’s health. Can dietary restriction modifications
provide significant improvement of the serological markers of
NAFLD - diagnosed patients?
Project Objective:
Demonstrate the methodology of evaluating whether dietary restriction of
Advanced Glycation Products (AGE’s) can significantly manage
Non-Alcoholic Fatty Liver Disease (NAFLD) based on simulated data.
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3. PROJECT OVERVIEW
PROCESS
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• Each stage will run for 14 days
• During the initial stage the patients will be educated about the
process and AGE’s.
• Screening tests will be carried out before and after the diet periods
Ex: measurement of height / weight / body fat

4. METHODOLOGY
Sample Size Determination
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5. METHODOLOGY
Data Simulation
• Simulate multivariate normal distribution for all serological markers
• Ignore non normality
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6. Analysis
• Random sampling
• Sample size > 30 - Central Limit Theorem (CLT) was invoked and proceeded
with the test
• Paired t- tests carried out to detect any significant difference in the means of
serological markers between different stages
 Baseline Vs Post-Low AGE diet test results
 Pre-High AGE diet Vs Post- High AGE diet test results
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METHODOLOGY

7. Expectations
Anthropometric Markers
 Low AGE’s Diet – No Change
 High AGE’s Diet – No Change
Metabolic Markers
 Low AGE’s Diet – No Change or Decrease
 High AGE’s Diet – Increase
Other Markers (Proteins, fat, etc.)
 Low AGE’s Diet – No Change or Decrease
 High AGE’s Diet – Increase
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METHODOLOGY

8. Analysis
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METHODOLOGY
Boxplots indicate
small difference in
means
Boxplots indicate
larger difference in
means

9. Results - Table
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METHODOLOGY
Hypothesis for paired sample t-test
𝝁 𝜟 = 𝟎
𝝁 𝜟 ≠ 𝟎
*𝝁 𝜟: 𝒑𝒐𝒑𝒖𝒍𝒂𝒕𝒊𝒐𝒏 𝒎𝒆𝒂𝒏
𝒐𝒇 𝒕𝒉𝒆 𝒅𝒊𝒇𝒇𝒆𝒓𝒆𝒏𝒄𝒆𝒔

10. Results - Graph
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METHODOLOGY

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BACKGROUND INFORMATION
CONCLUSIONS
Can dietary restriction modifications provide significant improvement of the
serological markers of NAFLD - diagnosed patients?
 Anthropometric Markers
Only “Waist” behaved as expected
 Metabolic Markers
None of the markers behaved as expected
 Other Markers
5 Out of 16 markers behaved as expected (“Platelet”, “ NAFLD
Fibrosis Score” , “Bili.Tot.” , “Bili.Unconj.” , “GGT” )

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BACKGROUND INFORMATION
CONCLUSIONS
 Only 6 out of 26 serological markers behaved as expected
 Dietary restriction modifications do not seem to have significantly
improved the NAFLD - diagnosed patients’ health.

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BACKGROUND INFORMATION
SUGGESTIONS
 Test of bivariate associations between different types of markers for each
stage.
 Patients could be given Pre-Prepared diets produced by the researchers.

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BACKGROUND INFORMATION
ACKNOWLEDGEMENT
RMIT University
- Dr Stelios Georgiou
- Kaye Marion
- Dr James Baglin
Austin Hospital
- Dr Chris Leung
- Laurence Alexander Jacuzzi

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