This document discusses resealed erythrocytes as a drug delivery system. It begins with an introduction to erythrocytes and how they can be used for drug delivery due to their ability to circulate throughout the body. It then describes how erythrocytes are isolated from blood and various methods for loading drugs into the erythrocytes, such as hypo-osmotic lysis and endocytosis. The document outlines advantages like biocompatibility and targeting specific tissues, as well as disadvantages like limited targeting of non-phagocyte tissues. It concludes that resealed erythrocytes show promise for drug delivery but require further optimization.
this presentation contain detail information of resealed erythrocytes from what are the resealed erythrocytes to where they are usesd? basic information like what are RBC and their role, history of resealed erythrocytes, sources and isolation method of erythrocytes,effect of tonicity on RBC's, method of drug loading in erythrocytes, characterization of them, applications
this presentation contain detail information of resealed erythrocytes from what are the resealed erythrocytes to where they are usesd? basic information like what are RBC and their role, history of resealed erythrocytes, sources and isolation method of erythrocytes,effect of tonicity on RBC's, method of drug loading in erythrocytes, characterization of them, applications
Resealed erythrocytes as a novel delivery carrierMariamZewail
Resealed erythrocytes are effective and safe drug carriers for targeted and sustained drug delivery. Drugs can be easily entrapped into erythrocytes by several techniques. Resealed erythrocytes can be used a carrier for drugs, enzyme replacement therapy etc. However, the concept needs further optimization to be converted into a regular drug delivery system.
Resealed erythrocytes as a novel delivery carrierMariamZewail
Resealed erythrocytes can be obtained due to the different responses of red blood cells in response to different pHs. Resealed erythrocytes have several applications as a drug or enzyme carrier.
Resealed erythrocytes as a novel delivery carrierMariamZewail
Resealed erythrocytes are effective and safe drug carriers for targeted and sustained drug delivery. Drugs can be easily entrapped into erythrocytes by several techniques. Resealed erythrocytes can be used a carrier for drugs, enzyme replacement therapy etc. However, the concept needs further optimization to be converted into a regular drug delivery system.
Resealed erythrocytes as a novel delivery carrierMariamZewail
Resealed erythrocytes can be obtained due to the different responses of red blood cells in response to different pHs. Resealed erythrocytes have several applications as a drug or enzyme carrier.
The study of the disposition of a drug
It includes the processes of ADME
Absorption
Distribution
Metabolism
Excretion
Toxicity
Patients may suffer
Toxic drugs may accumulate
Useful drugs may have no benefit because doses are too small to establish therapeutic efficacy
A drug can be rapidly metabolized
Absorption is the process by which a drug enters the bloodstream without being chemically altered
Factors which influence the rate of absorption
types of transport
the physicochemical properties of the drug
protein binding
routes of administration
dosage forms
circulation at the site of absorption
concentration of the drug
Resealed Erythrocytes:Introduction, Isolation of erythrocytes, Methods of drug loading, Applications of resealed erythrocytes, Advantages of erythrocytes as carriers, Disadvantages of erythrocytes as carriers,Recent developments and Future perspective.
1. Bio preservation of Red Cell Components 2. CULTURED RBCs 3. solvent plasmaDrShinyKajal
1. Bio preservation of Red Cell Components
2. CULTURED RBCs
3. Solvent plasma
Hypothermic storage
Cryopreservation
Lyophilization
Desiccated RBCs
Three major sources of cells are under consideration:
circulating stem and progenitor cells from adults or from cord blood
immortalized progenitors
pluripotent stem cells.
Immortalized Progenitors
LIQUID CULTURE METHODS- by the SED (stem cell factor (SCF), erythropoietin, and dexamethasone) and STIF cocktails (stem cell factor, thombopoietin, insulin-like growth factor-2, fibroblast growth factor-2)
ENUCLEATION- separation of extruded nuclei from cRBCs
SCALING UP- using cord blood CD34+ cells in bioreactors
and treated for 4 h with TNBP (tri-nitrobutylphosphate)solvent and with Triton X-100 detergent
Human Stem Cells- Introduction
Types of HSC transplants
Indications
Sources of stem cells
Collection and mobilization
Types of Mobilizing agents
Processing
Cryopreservation and storage
PBSC Transplant
Quality control
Complications
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
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Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
1. Prepared By-
Biplajit Das
Roll No-06
B.Pharm 4th Year(8th Semester)
Department Of Pharmaceutical Sciences
Dibrugarh University, Dibrugarh, Assam, 786004
2. CONTENTS
Introduction
Erythrocytes
Resealed Erythrocytes
Advantages & Disadvantages
Way of Use of Erythrocytes
Isolation Of Erythrocytes
Methods Of Drug Loading In Erythrocytes
Evaluation Of Resealed Erythrocytes
Application Of Resealed Erythrocytes
Conclusion
References
3. INTRODUCTION
Blood contains different type of cells like erythrocytes (RBC),
leucocytes (WBC) and platelets, among them erythrocytes are
the most interesting carrier and posses great potential in drug
delivery due to their ability to circulate throughout the body,
zero order kinetics, reproducibility and ease of preparation.
Primary aim for the development of this drug delivery system
is to maximize therapeutic performance, reducing undesirable
side effects of drug as well as increase patient compliance. The
overall process is based on the response of these cells under
osmotic conditions. Upon reinjection, the drug-loaded
erythrocytes serve as slow circulating depots and target the
drugs to disease tissue or organ.
4. ERYTHROCYTES
Red blood cells (also referred to as erythrocytes) are the
most common type of blood cells and the vertebrate
organism's principal means of delivering oxygen (O2) to
the body tissues via the blood flow through the circulatory
system.
The cells develop in the bone marrow and circulate for
about 100–120 days in the body before their components
are recycled by macrophages. Each circulation takes about
20 seconds. Approximately a quarter of the cells in the
human body are red blood cells.
5. RESEALED ERYTHROCYTES
Such drug-loaded carrier erythrocytes are prepared simply by
collecting blood samples from the organism of interest,
separating erythrocytes from plasma, entrapping drug in the
erythrocytes, and resealing the resultant cellular carriers.
Hence, these carriers are called resealed erythrocytes. The
overall process is based on the response of these cells under
osmotic conditions. Upon reinjection, the drug-loaded
erythrocytes serve as slow circulating depots and target the
drugs to a reticuloendothelial system (RES).
6. ADVANTAGES & DISADVANTAGES
1. Biocompatible, particularly when autologous cells are used hence no
possibility of triggered immune response.
2. Biodegradability with no generation of toxic products.
3. Considerable uniform size and shape of carrier.
4. Relatively inert intracellular environment can be encapsulated in a
small volume of cells.
5. Isolation is easy and large amount of drug can be loaded.
6. Prevention of degradation of the loaded drug from inactivation by
endogenous chemical.
7. Entrapment of wide variety of chemicals can be possible.
8. Entrapment of drug can be possible without chemical modification
of the substance to be entrapped.
9. Possible to maintain steady-state plasma concentration, decrease
fluctuation in concentration.
7. 10. Protection of the organism against toxic effect of drug.
11. Targeting to the organ of the RES.
12. Ideal zero-order drug release kinetic.
13. Prolong the systemic activity of drug by residing for a longer
time in the body
Disadvantages:
1. They have a limited potential as carrier to non-phagocyte target
tissue.
2. Possibility of clumping of cells and dose dumping may be
there.
CONTD….
8. Erythrocytes can be used as carriers
in two ways
1. Targeting particular tissue/organ
For targeting, only the erythrocyte membrane is used. This is
obtained by splitting the cell in hypotonic solution and after
introducing the drug into the cells, allowing them to reseal into
spheres. Such erythrocytes are called Red cell ghosts.
2. For continuous or prolonged release of drugs.
Alternatively, erythrocytes can be used as a continuous or
prolonged release system, which provide prolonged drug
action. There are different methods for encapsulation of drugs
within erythrocytes. They remain in the circulation for
prolonged periods of time (up to 120 days) and release the
entrapped drug at a slow and steady rate.
9. ISOLATION OF ERYTHROCYTES
Blood is collected into heparin zed tubes by venipunture.
Blood is withdrawn from cardiac/splenic puncture (in small animal)
and through veins (in large animals) in a syringe containing a drop of
anti-coagulant.
The whole blood is centrifuged at 2500 rpm for 5 min. at 4 ±10C in
a refrigerated centrifuge.
The serum and Buffy coats are carefully removed and packed cells
washed three times with phosphate buffer saline (pH=7.4).
The washed erythrocytes are diluted with PBS and stored at 40C for
as long as 48 h before use.
Various types of mammalian erythrocytes
have been used for drug delivery, including erythrocytes of mice,
cattle, pigs, dogs, sheep, goats, monkeys, chicken, rats, and rabbits.
10. METHODS OF DRUG LOADING IN
ERYTHROCYTES
1. Hypo-osmotic lysis Method
*Dilution Method
*Dialysis Method
*Pre-swell Method
*Isotonic Osmotic Lysis Method
2. Electric Breakdown Method
3. Endocytosis Method
4. Membrane Perturbation method
5. Normal Transport method
6. Lipid Transfusion Method
11. DILUTION HAEMOLYSIS
RBC Membrane ruptured Loaded RBC
Efficiency is 1-8%
Resealing
buffer
Incubation
at 25°C
Resealed
Loaded
RBC
0.4% Nacl
Hypotonic
Drug
Loading buffer
15. DIALYSIS METHOD
RBC + Phosphate buffer Placed in dialysis bag with air bubbles
Dialysis bag placed in the 200 ml of lysis buffer with mechanical rotator 2 hrs
Loaded RBC
Dialysis bag placed in resealing buffer with mechanical rotator 30 min at 37°C
Resealed RBC
drug Loaded
buffer
17. EVALUATION OF RESEALED ERYTHROCYTES
After loading of therapeutic agent on erythrocytes, the carrier cells are exposed to physical, cellular as
well as biological evaluations.
1. Shape and Surface Morphology
The morphology of erythrocytes decides their life span after administration. The morphological
characterization of erythrocytes is undertaken by comparison with untreated erythrocytes using either
transmission (TEM) or Scanning electron microscopy (SEM). Other methods like phase contrast
microscopy can also be used.
2. Drug Content
Drug content of the cells determines the entrapment efficiency of the method used. The process involves
deproteinization of packed, loaded cells (0.5 mL) with 2.0 mL acetonitrile and centrifugation at 2500 rpm
for 10 min. The clear supernatant is analyzed for the drug content by spectrophotometrically.
3. Cell Counting and Cell Recovery
This involves counting the number of red blood cells per unit volume of whole blood, usually by using
automated machine it is determined by counting the no. of intact cells per cubic mm of packed
erythrocytes before and after loading the drug.
4. Turbulence Fragility
It is determined by the passage of cell suspension through needles with smaller internal diameter (e.g., 30
gauges) or vigorously shaking the cell suspension. In both cases, haemoglobin and drug released after the
procedure are determined. The turbulent fragility of resealed cells is found to be higher.
18. Applications of Resealed Erythrocytes
Slow Drug Release
Drug Targeting
* Targeting Reticulo Endothelial System (RES) Organs
*Targeting the Liver Enzyme Deficiency/ Replacement
Therapy
Treatment of Hepatic Tumors
Removal of Toxic Agents
Delivery of Antiviral Agents
Enzyme Therapy
Improvement in Oxygen Delivery to Tissues
Osmotic shock
19. CONCLUSION
The use of resealed erythrocytes looks promising for a safe
and sure delivery of various drugs for passive and active
targeting. However, the concept needs further optimization to
become a routine drug delivery system.
The same concept also can be extended to the delivery of
biopharmaceuticals and much remains to be explored regarding
the potential of resealed erythrocytes. During the past decade,
numerous applications have been proposed for the use of
resealed erythrocytes as carrier for drugs, enzyme replacement
therapy etc. Until other carrier systems come of age, resealed
erythrocytes technology will remain an active arena for the
further research.
20. REFERENCES
Kumar Ritesh , Chandra Amrish, gautam Pawan
Kumar, “Resealed Erythrocytes As A Novel Carrier For
Drug Delivery”, International Journal Of Science And
Pharmceutical Research,2013.5.280-292
Raut B. Deepika , Sakhare S. Ram Dadge , K. Ketan,
Halle PD, “Resealed Erythrocytes Drug Delivery”,
International Journal Of Reseach In Pharmacy And
Chemistry,2013,3(2).198-207