This document discusses the potential role of regenerative medicine in the next 100 years. It outlines several approaches being researched like in vivo organ regeneration using stem cell transplantation and differentiated induced pluripotent stem cells. Ex vivo organ regeneration techniques are also discussed, including decellularizing and reseeding pig lungs with human cells. The document suggests that within a decade, significant advancements could be made in areas like xenotransplantation and robotic transplantation to further regenerative medicine goals.

1. The
Role
of
Regenera-ve
Medicine
in
the
100YSS
Contribu-on
128,
ID
1172
100YSS
Conference,
Houston
TX
2012
Sept
15
Mar-ne
RothblaF,
mar@Unither.com
United
Therapeu-cs
Corpora-on
100YSS
Regenera-ve
Medicine
mar@Unither.com
2

2. Regenera-ve
Medicine
in
Film
100YSS
Regenera-ve
Medicine
mar@Unither.com
3

3. Supernovas
(eg
Crab
Nebula)
èGalac-c
Cosmic
Rays,
With
The
Ejected
Protons
Ricoche-ng
Like
Billiard
Balls
Against
Galaxy’s
Magne-c
Field
100YSS
Regenera-ve
Medicine
mar@Unither.com
4

4. Gamma
Rays
Produced
when
Ubiquitous
Galac-c
Cosmic
Rays
Interact
with
Interstellar
Medium
Reveals
100YSS
Regenera-ve
Medicine
mar@Unither.com
5

5. Organ
Transplants
Extend
Life
100YSS
Regenera-ve
Medicine
mar@Unither.com
6

6. 100YSS
Regenera-ve
Medicine
mar@Unither.com
7
In
Vivo
Organ
Regenera-on
Via
Stem
Cell
Transplanta-on
1st
Genera-on
iPS
Technology

7. 100YSS
Regenera-ve
Medicine
mar@Unither.com
8
In
Vivo
Organ
Regenera-on
Via
Stem
Cell
Transplanta-on
2nd
Genera-on
iPS
Technology

8. Transplantable
Differen-ated
iPS
Cells
100YSS
Regenera-ve
Medicine
mar@Unither.com
9

9. In
Vivo
Liver
Regenera-on
100YSS
Regenera-ve
Medicine
mar@Unither.com
10

10. 100YSS
Regenera-ve
Medicine
mar@Unither.com
11

11. Rat
Lungs
Can
Be
Kept
Breathing
in
a
Fluid-­‐Air
System
for
a
Week
100YSS
Regenera-ve
Medicine
mar@Unither.com
12

12. Decellularized
Pig
Lungs
100YSS
Regenera-ve
Medicine
mar@Unither.com
13

13. Ex
Vivo
Organ
Regenera-on
for
Transplanta-on
(Pig
Lungs)
100YSS
Regenera-ve
Medicine
mar@Unither.com
14

14. Human
Peripheral
Endothelial
Progenitor
Cells
Can
Be
Cultured,
Manipulated
&
SEEDED
Human
Umbilical
Vascular
Endothelial
Cells
Used
To
Date
100YSS
Regenera-ve
Medicine
mar@Unither.com
15

15. Timeframe
Considera-on
• Human
Lung
Has
About
109
Endothelial
Cells
• Within
a
Month,
Endothelial
Progenitor
Cells
Found
in
Peripheral
Circula-on
Can
be
Cultured
to
Around
106
• Doubling
Time
of
Around
30
Days
• May
Require
About
a
Year
to
Grow
Lungs
for
Transplant
Once
Decide
to
Proceed
100YSS
Regenera-ve
Medicine
mar@Unither.com
16

16. Xenogeneic
Organ
Regenera-on
17
100YSS
Regenera-ve
Medicine
mar@Unither.com

17. Xenogeneic
Pigs
on
O’Neill
Habitat
100YSS
Regenera-ve
Medicine
mar@Unither.com
18

18. hEPCR
hVWF
hCD73
pVWFKO
hTFPI
hTBM
hCD39
19
Coagulate
GP1b
GP2b3q
GTKO
hMCP
HLA-­‐E
hDAF
hMIRL
Immune
SIRP/
CD47
Lec-ns
Sialyda-on
Selec-ns
100YSS
Regenera-ve
Medicine
mar@Unither.com

19. Da
Vinci
Surgical
Robot
100YSS
Regenera-ve
Medicine
mar@Unither.com
20

20. Da
Vinci
Robots
Have
Now
Explanted
Livers
for
Transplanta-on
100YSS
Regenera-ve
Medicine
mar@Unither.com
21

21. Robo-c
Transplanta-on
on
100YSS
of
Xenograis
or
Regen
Organs
Will
Be
Prac-cal
100YSS
Regenera-ve
Medicine
mar@Unither.com
22

22. Just
Like
Shepard’s
May
1961
Flight
Was
<.1%
to
the
Moon
23
100YSS
Regenera-ve
Medicine
mar@Unither.com

23. We
Can
Be
1000x
Further
Than
We
Are
In
Under
Ten
Years!
24
100YSS
Regenera-ve
Medicine
mar@Unither.com