Raman spectroscopy is a non-destructive technique that provides chemical structure information by analyzing scattered light. It works by shining a monochromatic light source, usually a laser, onto a sample, and detecting the scattered light. The scattered light may have different wavelengths than the incident light due to molecular vibrations. The instrumentation includes a wavelength selector to separate Raman scattered light from the laser light, and a transducer to convert the scattered light into an electrical signal for analysis. Raman spectroscopy has advantages like being applicable to any state of matter and only requiring a small sample size, but also limitations such as not working for metals and samples being at risk of heating from lasers. Its applications include inorganic material analysis, biology, forensics

1. KONGU
ENGINEERING
COLLEGE
Presented by:
MUTHU VENKATESH V (23MER022)
SANJAY PRAKASH D S(23MER038)
Date: 05/01/2024

2. Raman
Spectroscopy

3. • According to Raman effect when a certain frequency of
monochromatic radiations incident on a sample, the incident light
is, the frequency of scattered light is different from that of the
incident light.

5. Raman Spectroscopy is a non-destructive analysis technique
that provides detailed information about atoms such as
chemical structure, polymorphy, crystallinity and molecular
interactions.

6. INSTRUMENTATION

9. Wavelength Selector:
• Separates Raman scattered light from the laser light.
• Use special filters to pick desired wavelengths.
Transducer:
• The transducer captures the scattered light and converts it into an
electrical signal that can be further processed and analyzed.
• Commonly used transducers in Raman spectroscopy include
photomultiplier tubes (PMTs) and charge-coupled devices (CCDs).

10. Advantages
• Can be used on any state of matter.
• Non-destructive testing.
• Highly specified to a molecule.
• Only need of a small quantity of sample.
• It is a quick analysis.

11. Limitations
• Not suitable for metal alloy.
• Expensive process
• The sample may get heated when it is exposed to a laser too long.
• Fluorescence often contaminates the Raman signal.

12. Applications
• Investigating inorganic systems.
• To study microscopic biological samples.
• Used to detect explosives.
• Used in forensics to analyse gun residuals.
• distinguish healthy and unhealthy tissue.

13. Thank You