This document contains the resume of Santosh Rama Bhadra Rao. It summarizes his contact information, educational qualifications, including a Master's degree in Biotechnology and postgraduate diplomas. It also lists his expertise in areas like molecular biology, tissue culture, cell biology, biochemistry, and bioinformatics. It provides details of his accomplishments, publications, experience in internships and as a Junior Research Fellow, seminars and conferences attended, and references.

1. TATA SANTOSH RAMA BHADRA RAO
MOBILE: +91 9494905949, +91 8523892902 E-MAIL:santosh.sanocito@gmail.com
Jawaharlal Nehru Technological University,
Kukatpally,
Hyderabad-85
India.
“Highly enthusiastic biology student ready to work in an innovative research”
Education
Masters (Biotechnology) - Jawaharlal Nehru Technological University (2012-2014), Project
(GMO’s) –ICRISAT - 81.63%
PG Diploma (Bio informatics) - Osmania University (2009- 2010)- Project (Pyridone derivatives)-
68%
Bachelors (Biotechnology)- Andhra University (2007- 2009)- Project (Water)-72%
Professional Overview
Expertise with Cell and Molecular biology techniques, animal cancer cell lines maintenance,
cloning, structural and functional analysis of biomolecules through in-vitro and in-silico
approach.
Likely to work in cancer biology, interactive genomic biology, cell and molecular biology and
microbiology.
Ambitious to handle multi-task-oriented programs at all levels.
Flexible and ready to work hard in any time.
Reveal the analytical expectations every time with positive results.
Habituate to work in deadline-oriented projects with highly motivated and maturely organized
research tasks.
Accomplishments
2nd rank holder at university level in Masters with 81.63%. (JNTUH)
Got consolation prize for poster presentation on the subject gene therapy and DNA mind
printing technology at CONGREGATION OF NOBLE LAUREATE at IIIT ALLAHABAD-2013.
Selected as an intern in cell and molecular biology lab at ICRISAT an international institute.
At bachelor's level got 1st rank at university level 72%. (Andhra University).
Working as a JRF on molecular cloning and characterization of eIF4A gene.

2. Core qualification
Molecular Biology:
Gene isolation, Restriction digestion, Vector
designing, Cloning, Primer designing,
Promoter analysis, Gene structure analysis,
PCR, SOUTHERN BLOTTING, ELISA,
Chromosomal mapping. Immuno and
protein assay studies to estimate expression
levels of protein and presence of protein
Tissue Culture:
Breast cancer & cervical cancer cell lines
maintenance and tripsinisation, animal cell
culture revival and maintenance in aseptic
conditions, Observation of the cell motility
and its development. Explants co cultivation
and explants regeneration, Electroporation
of bacterial cells, Transformation, Microbe’s
isolation revival and culture maintenance.
Chemical and electro Competent cell
preparation
Cell biology:
Microscope handling, Staining techniques,
Observations of cells and its cell organelles.
(Chloroplast, Mitochondria)
Biochemical:
TLC, SDS PAGE, Electrophoresis,
Chromatography, Acid Hydrolysis,
Extraction of compounds from raw
materials, DNA, RNA, Protein and lipid
isolation and separation, estimation, and
conformation tests.
In-Silico:
Drug designing and docking of molecules,
data retrieval and analysis, using various bio
informatics tools. Done with publication
paper setting work on research oriented
basis.
Bioprocess Engineering:
Enzymes extraction from micro organisms
and fermentation technology.
Enzyme beads technology, immobilizations
of enzyme estimation of enzyme activity
and structural analysis.
THESIS/DESERTRATION
Molecular cloning and characterization of full length cDNA clones of Peanut Bud Necrosis virus (MSc)-
Guide- Dr KK Sharma.
Expertise in gene cloning into a vector and transformation. Performed tissue culture and
transformation procedures towards transgenic Groundnut generation.
Done with confirmations of generated transgenics through polymerase chain reaction and ELISA
techniques.
3d QSAR studies on pyridone derivatives molecular characterization through in-silico approach (PG
Diploma).Guide- Prof PB Kavi kishore
Expertise with Homology modeling, Blast, Phylogentic tree generation and analysis secondary
structure predictions using Sybyl and Modeller. Drug designing and docking.
Chemical nature of water and its availability forms in living things-(BSc)

3. Experience
2012 0ct- 2013 March - worked on Breast cancer cell lines revival and maintenance for research
study. Analyzed the chromosome and cell organelles motility during the diseased state.
2013 May- 2014 Jan - done with internship at ICRISAT for transgenic cell lines also experienced
with hydroponics experiment.
Working as a JUNIOR RESEARCH FELLOW under RKVY project cloning and characterization of
eIF4A gene.
Publications:
Genome-wide identification and characterization of the aquaporin gene family in Sorghum
bicolor (L.)
Palakolanu Sudhakar Reddy, Tata Santosh Rama Bhadra Rao, Kiran K. Sharma, Vincent Vadez
Plant Gene, Volume 1, March 2015, Pages 18-28.
In addition:
Wrote a book ABIOGENERA OF SPECIES waiting to publish with my professor after PhD work
along with my dream project studies.
Seminars/Conferences
Worked as an organizing committee member and trainer for a DBT sponsored training
programme on Molecular Cloning and Transgenic technologies at Agri Biotech Foundation in
1/09/2015 TO 16/09/2015.
Worked as an organizing committee member and trainer for a DBT sponsored work shop on
drug discovery and molecular docking analysis along with bio informatics tools handling for
molecular biology at Osmania University in December 2014 from 24/12/2014 to 31/12/2014.
Worked as an organizing committee member and trainer for an international work shop on
molecular cloning and RNAi approach for disease resistant transgenic technology at ICRISAT in
December 2013.
Poster presented on GENOME-MECHANICS with the subject representing Advanced gene
therapy for cancer, diabetes and DNA mind printing technology at Congregation Of Noble
Laureates- IIT Allahabad 2013 from 11/12/2013 to 20/12/2013.
Participated in national conference DNA 2012 on DNA finger printing technology at University Of
Hyderabad in 2012 December from 2/12/2012 to 4/12/2012.

4. References
a) Prof. M. Laksmi Narsu
Head of the department, Department of
Biotechnology
Jawaharlal Nehru Technological University,
Kukatpally, Hyderabad-500 085, India.
Email: mangamoori@jntuh.ac.in
b) Dr. Palakolanu Sudhakar Reddy
Scientist (DST-Inspire Faculty Fellow),
Dryland Cereals, International Crops
Research Institute for the Semi-Arid
Tropics (ICRISAT)
Patancheru- 502 324, Hyderabad.
Telangana, INDIA
Ph: +91-40-30713594
Fax: +91-40-30713074
p.sudhakarreddy@cgiar.org
palakolanusreddy@gmail.com
palakolanusreddy@daad-alumni.de
c) Dr. Bokara Kiran Kumar
Scientist
CSIR-Center for Cellular and Molecular
Biology, (CSIR-CCMB), ANNEX-2, Clinical
Research Facility-Medical Biotechnology
(CRF-MB), Uppal, Hyderabad-500 007, India.
Phone: +91-040-2719-5547,
Mobile: +91-91778 74028
FAX: +91-40-2716 0591, 2716 0311,
Email: bokarakiran@ccmb.res.in,
bokarakiran@gmail.com