This study monitored the aggregation of compounds like food dyes using NMR spectroscopy at increasing concentrations. Some compounds showed broad resonance peaks and unexpected peaks, indicating aggregation. As concentration increased, peaks shifted and became better defined. The detergent Tween 20 disrupted aggregates, changing peak shapes. Certain food dyes have been linked to adverse health effects like reduced organ function and cancer risks in previous studies. This NMR assay allows detection of compound aggregation that could contribute to undesirable side effects.