1. A DNA construct is an artificially constructed segment of nucleic acid that is
going to be "transplanted" into a target tissue or cell. It often contains a DNA
insert, which contains the gene sequence encoding a protein of interest, that
has been subcloned into a vector, which contains bacterial resistance genes for
growth in bacteria, and promoters for expression in the organism. A DNA
construct may express wildtype protein, prevent the expression of certain genes
by expressing competitors or inhibitors, or express mutant proteins, such as
deletion mutations or missense mutations. A DNA construct is often used in
molecular biology to analyze macromolecules such as proteins or RNA in more
detail.
DNA
construct

2. This pcDNA™3.1(+) vector is designed for high-level, constitutive
expression in a variety of mammalian cell lines. It contains a Geneticin®
selectable marker and a forward-orientation multiple cloning site.
Adjuvant:
a substance which enhances the body's immune response to an antigen.
M cells
special epithelial cells associated with Peyer's patches and lymphoid follicles
that actively take up particulate matter from the intestinal contents. They are
probably the portal of entry for bacteria and viruses.

3. Ovalbumin
The biological function of ovalbumin is unclear although it is said to act as a storage
protein. In research, the ovalbumin (of chicken egg) is used as a molecular weight
marker for calibrating electrophoresis gels. It is also used to stimulate allergic
reaction in test subjects. In medicine, it is administered to patients suspected of
being poisoned by heavy metals (e.g. iron).
Caco-2 cells
The human intestinal Caco-2 cell line originally isolated by
J. Fogh (Sloan Kettering Institute, New York, NY) from a human co-lon
adenocarcinoma (Fogh et al., 1977), is still the best available
cell culture model of absorptive small intestinal enterocytes (Delie
and Rubas, 1997; Le Ferrec et al., 2001; Sambruy et al., 2001) and it
is extensively utilized for toxicological and pharmacological stud-ies.
Transfection
Transfection is the process of deliberately introducing nucleic acids into cells. The
term is often used for non-viral methods in eukaryotic cells

4. Fluorescein isothiocyanate (FITC)
a fluorochrome dye frequently coupled to antibodies that are used to locate and
identify specific antigens.
PLGA or poly(lactic-co-glycolic acid) is a copolymer which is used
in a host of Food and Drug Administration
Poly(lactic-co-glycolic acid) (PLGA) is one of the most successfully
used biodegradable polymers because its hydrolysis leads to metabo-lite monomers,
lactic acid and glycolic acid ( Fig. 1). Because these two
monomers are endogenous and easily metabolized by the body via
the Krebs cycle, a minimal systemic toxicity is associated with the
use of PLGA for drug delivery or biomaterial applications