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Strain improvement through genetic engineering

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Strain improvement through genetic engineering

  1. 1. Presented by: 1. Sulov Saha 2. Mehedi Hasan Strain Improvement through Genetic Engineering
  2. 2. A strain is a subgroup of a species with one or more characteristics that distinguish it from other subgroups of the same species. Each strain is identified by a name, number, or letter. For example: L. acidophilus LA-5 What is Strain?
  3. 3. Strain Improvement The science and technology of manipulating and improving microbial strains, in order to enhance their metabolic capacities for biotechnological applications, are referred to as Strain Improvement.
  4. 4. Objectives to get multiple copies of specific gene to get high amounts of specific protein or product to integrate gene of interest of one organism into another
  5. 5. Strategies for Strain Improvement through Genetic Engineering 1. Sources of DNA 2. Vector 3. Hosts 4. Metabolic Engineering
  6. 6. Mechanism
  7. 7. Steps involved Preparation of desired DNA Insertion of desired DNA into vector DNA Introduction of recombinant DNAs into host cells Identification of recombinants Expression of cloned genes
  8. 8. Applications In the field of food technology: production of recombinant enzyme In the field of agriculture: recovery of plant’s characters In the field of microbiology: improve the microbe’s productivities or characteristics In the industry: improvement of industrial strain (e.g. paper industry)
  9. 9. Also known as rennin Single polypeptide chain of 323 amino acids Main coagulating enzyme found in rennet which is used extensively in cheese production. Increase Chymosin production has been made through expression of calf Chymosin gene in recombinant K. lactis. Case Study Chymosin
  10. 10. Case Study (cont.)
  11. 11. Future Aspects Industrial strains Food Engineering System Biology Desired traits Biodiversity Strain Improvement through Genetic Engineering will undoubtedly continue as the knowledge about the genetic make-up of microorganisms used for enzyme production expands and new genetic techniques emerge.
  12. 12. Recombinant DNA methods have been especially useful in the production of primary metabolites such as amino acids, but are also finding increasing use in strain development programs for antibiotics. Current strain improvement strategies have already contributed to creating more efficient and safer enzyme production strains. The task of both discovering and improving the new strain of interest ones have become more and more challenging. Conclusion

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